XU Jing-ting,REN Jian-hua,YE Ling-feng,et al.Technical Research of Industrialized 7S and 11S Soy Protein Fractionation[J].Soybean Science,2010,29(03):519-524,529.[doi:10.11861/j.issn.1000-9841.2010.03.0519]
工业化大豆蛋白7S和11S组分分离技术研究进展
- Title:
- Technical Research of Industrialized 7S and 11S Soy Protein Fractionation
- 文章编号:
- 1000-9841（2010）03-0519-06
- Keywords:
- Soy protein; Separation techniques; Industrial production; 7S rich fraction; 11S rich fraction; Yield; Purity.
- 分类号:
- TS214.2
- 文献标志码:
- A
- 摘要:
- 该文围绕目前工业化生产11S和7S组分的分离技术成果，结合实验室的分离技术（Guo法），比较了各种分离方法的差异。结果表明：分离提取技术利用的原理几乎均建立在“碱溶酸提”和“冷沉”作用基础之上。Wu首次成功地进行了7S和11S的工业化分离，之后的研究多是对此方法的改进和修饰。而Guo法是唯一采用中性条件抽提的工业化分离方法，在节省了原料碱的同时，避免了蛋白的变性。同时，该文提出了目前工业化生产大豆组分蛋白中存在的问题，虽然不断在加工方式上进行改进，但仍存在步骤复杂、产率低、生产成本高等方面的问题，因此作者进一步对实现工业化分离大豆组分蛋白提出了需要改进和努力的方向。
- Abstract:
- Combining with our isolation techniques (method of Guo), this article compared different separation methods of 11S and 7S fractions with industrial methods. The principles of most separation techniques are “alkali extraction and acid precipitation” and “cold precipitation” effects. Wus method succeeded in isolation 11S and 7S fraction for the first time, the later researches mostly focus on its improvement. The method of Guo is the only method to extract protein in a neutral condition, thus saved the usage of alkali and avoided the denaturation of protein. Meanwhile, this article pointed out the problems in the current industrial production. Although lots of improvements were made on the separation process, problem still exist, such as complicated procedures, low yields, high costs of production and so on. Therefore, the writer further proposed the directions of efforts and improvement for the realization of industrial production.
参考文献/References:
[1]Mujoo R, Trinh D T, Ng P K W. Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture[J]. Food Chemistry, 2003, 82: 265-273. [2]Riblett R C, Herald T J. Characterization of β-conglycinin and glycinin soy protein fractions from four selected soybean genotypes[J]. Journal of Agricultural and Food Chemistry, 2001, 49: 4983-4989. [3]Silvana P, Maria C A. Soy protein isolate components and their interactions [J]. Journal of Agricultural and Food chemistry, 1995, 43: 1762-1767. [4]Iwabuchi S. Thermal denaturation of β-conglycinin[J]. Journal of Agricultural and Food chemistry, 1991, 39 (1): 27-32. [5]Kitamura K, Takagi T, Shibasaki K. Subunit structure of soybean 11S globulin [J]. Agricultural and Biological Chemistry, 1976, 40 (9): 1837-1844. [6]Utsumi S, Inaba H, Mori T. Heterogeneity of soybean glycinin [J]. Phytochemistry, 1981, 20: 585-589. [7]Badley R A, Atkinson D. The Structure, physical and chemical properties of the soybean protein glycinin [J]. Biochimica et Biophysica Acta, 1975, 41: 224-228. [8]Saio K, Watanabe T. Differences in functional properties of soybean proteins [J]. Journal Texture Studies, 1978, 9: 1335-1357. [9]Altschul A M, Wilcke H L. New protein foods-seed storage proteins [G]. Academic Press, 1985:5. [10]Shi Y G, Ren L. Technology of soybean products [M]. Beijing: Light Industry Press Inc，1993. [11]Koshiyama I. Factors influencing conformation changes in a 7S protein of soybean globulins by ultracentrifugal inveatigations[J]. Agricultural and Biological Chemistry, 1968, 32：879-887. [12]Maruyama N, Katsube Y. The role of N-linked glycans and extension regions of soybean β-conglycinin folding, assembly and structural features [J]. European Journal of Biochemistry, 1998, 258: 854-862. [13]Fukushima D. Recent progress of soybean protein foods: Chemistry [J]. Technology and Nutrition Food Reviews International, 1991, 7 (3): 323-351. [14]Youyan Sen. Formation mechanism of soybean protein gel[J]. Nippon Nogeikagaku Kaishi, 1988, 62(5): 882-885. [15]Zhou R B. Structure and function of 7S and 11S protein[J]. Journal of the Chinese Cereals and Oils Association, 1998, 13, (6): 39-42. [16]Chen H M, Hua Y F. The study of soy protein compostion and fountion[J]. China Western Cereals & Oils Technology, 2001, 26 (3): 36-38.(in Chinese with English abstract) [17]Hu C, Huang L H, Li W Z. The ratio of 11S/7S globulin impacts soybean proteins fountions[J]. Journal of the Chinese Cereals and Oils Association, 2004, 19 (1): 40-42.(in Chinese with English abstract) [18]Yang B C, Zhang X M, Guo S T. Glycopeptide derived from soybean β-conglycinin inhibits the adhesion of Escherichia coli and Salmonella to human intestinal cells [J]. Food Research International, 2008, 41: 594-599. [19]Yang B C, Lu Y. The inhibitory action of soybean β-conglycinin hydrolysates on Salmonella typhimurium translocation in Caco-2epithelial cell monolayers[J]. Journal of Agricultural and Food chemistry, 2008, 56: 7522-7527. [20]Shen C L, Chen W H, Zou S X. In vitro and in vivo effects of hydrolysates from conglycinin on intestinal microbial community of mice after Escherichia coli infection[J]. Journal of Applied Microbiology, 2007, 102(1): 283-289. [21]Manzoni C, Duranti M, Eberini I,et al. Subcellular localization of soybean 7S globulin in HepG2cells and LDL receptor up-regulation by its alpha?constituent subunit[J]. Journal of Nutrition, 2003, 133: 2149-2155. [22]Duranti M, Lovati M R, Dani V, et al. The alpha?subunit from soybean 7S globulin lowers plasma lipids and upregulates liver beta-VLDL receptors in rats fed a hypercholesterolemic diet[J]. Journal of Nutrition, 2004, 134: 1334-13349. [23]Aoyama T, Kohno M, Saito T, et al. Reduction by phytate-reduced soybean β-conglycinin of plasma triglyceride levels of young and adult rats[J]. Bioscience Biotechnology & Biochemistry, 2001, 65: 1071-1075. [24]Baba T, Ueda A, Kohno M et al. Effects of soybean beta-conglycinin on body fat ration and serum lipid levels in healthy volunteers of female university students[J]. Journal of Nutritional Science and Vitaminology, 2004, 50: 26-31. [25]Moriyama T, Kishimoto K, Nagai K, et al. Soybean beta-conglycinin diet suppresses serum triglyceride levels in normal and genetically obese mice by induction of beta-oxidation, downregulation of fatty acid synthase, and inhibition of triglyceride absorption[J]. Bioscience Biotechnology & Biochemistry, 2004, 68: 352-359. [26]Wolf W J, Babcock G E, Smith A K. Purification and stability studies of the 11S component of soybean proteins[J]. Archives of Biochemistry and Biophysics, 1962, 99: 265-274. [27]Wolf W J, Sly D A. Cryoprecipitation of soybean 11S protein[J]. Cereal Chemistry, 1967, 44: 653-668. [28]Wolf W J. Physical and chemical properties of soybean proteins[J]. Journal of the American Oil Chemists′ Society, 1997, 54 (2): 112-117. [29]Thanh V H, Okubo K, Shibasaki K．Isolation and characterization of the multiple 7S globulins of soybean[J]. Plant Physiology, 1975, 56: 19-22. [30]Thanh V H, Okubo K, Shibasaki K. Major proteins of soybean seeds a straightforward fraction and their characterization heterogeneity of Beta-conglycinin[J]. Journal of Agricultural and Food chemistry, 1976, 24: 1117-1121. [31]Deak N A, Murphy P A, Johnson L A. Effects of reducing agent concentration on soy protein fractionation and functionality[J]. Journal of Food Science, 2006, 71(3): C200-C208. [32]Deak N A, Murphy P A, Johnson L A. Effects of NaCl concentration influence on β-conglycinin yield and purity during soy protein fractionation[J]. Journal of Food Science, 2006, 71(4): C247-C254. [33]Wu S, Murphy P A, Lawrence A J, et al. Pilot-plant fractionation of soybean glycinin and β-conglycinin[J]. Journal of the American Oil Chemists′ Society, 1999, 76: 285-93. [34]Nagano T, Mtohiko H, Hiroyuki M, et al. Dynamic viscoelastic study on the gelation of 7S globulin from soybeans[J]. Journal of Agricultural and Food Chemistry, 1992, 48: 941-944. [35]Wu S, Patricia A M, Lawrence A J, et al. Simplified Process for Soybean Glycinin and β-conglycinin in Fractionation[J]. Food Chemistry, 2000, 48(7): 2702-2708. [36]Rickert D A, Johnson L A, Murphy P A. Improved fractionation of glycinin and β-conglycinin and partitioning of phytochemicals[J]. Journal of Agricultural and Food chemistry, 2004, 52: 1726-34. [37]Guo S T, Han Y J, Meng Y, et al. A method for extracting soybean protein[P]. China Patent,200410029589.4.
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备注/Memo
Biography:XU Jing-ting(1983-),PhD,engaged in the research of protein processing and utilization.