LI Jing,XIN Guang,ZHANG Bo,et al.Comparison between Simultaneous Distillation Extraction and Solid-phase Micro-extraction Methods for Analysis of Volatile Constituents in Brine Dried Tofu[J].Soybean Science,2010,29(03):502-505.[doi:10.11861/j.issn.1000-9841.2010.03.0502]
同时蒸馏萃取和固相微萃取分析卤水干豆腐挥发性成分比较
- Title:
- Comparison between Simultaneous Distillation Extraction and Solid-phase Micro-extraction Methods for Analysis of Volatile Constituents in Brine Dried Tofu
- 文章编号:
- 1000-9841（2010）03-0502-04
- 关键词:
- 同时蒸馏萃取; 固相微萃取; 卤水干豆腐; 气相色谱-质谱法（GC-MS）
- Keywords:
- Solid-phase Micro-extraction; Simultaneous Distillation and Extraction; Brine dried tofu; Gas Chromatography-mass Spectrometry
- 分类号:
- TS214.2
- 文献标志码:
- A
- 摘要:
- 采用同时蒸馏萃取法和固相微萃取法提取卤水干豆腐中的挥发性成分，并利用GC-MS进行分离鉴定。对2种方法所鉴定出的挥发性成分进行比较，确定出最适合卤水干豆腐挥发性成分萃取的方法。结果表明：同时蒸馏萃取法能更有效的提取出干豆腐的挥发性成分（53种），而且有利于高沸点成分的分离。固相微萃取技术尽管分析出的成分数量较少（25种），但它具有快速简便、不使用溶剂和不破坏检测样品等优点，能反映卤水干豆腐的原始信息，可用于挥发性成分的初步鉴定。2种方法共同鉴定出的卤水干豆腐的挥发性成分有己醛、1-己醛、苯乙烯、正庚醛、2-正戊基呋喃、辛醛、安息香醛、1-辛烯-3-醇和壬醛。
- Abstract:
- Volatile constituents of Brine dried tofu extracted by Simultaneous Distillation Extraction(SDE) and Solid-phase Micro-extraction(SPME) method were identifed by Gas Chromatography-mass Spectrometry(GC-MS), to determine the suitable method for extracting the volatile constituents from brine dried tofu. For SDE method, 53 volatile constituent were extracted and it is conductive to separate the constituents with high boiling point. For SPME method, 25 volatile constituent were extracted, although the quantity of volatile constituents is smaller compared with SDE method, it can reflect the original information of brine dried tofu and can be used for preliminary identification of volatile constituents with the advantage of easy to operate, does not use solvents or destroy the testing samples. The common volatile constituents in brine dried tofu identified by the two methods are Hexanal, 1-Hexanol, styrene,Heptanal, Furan,2-pentyl-, Octanal, Benzaldehyde，1-octen-3-ol and Nonanal.
参考文献/References:
[1]李里特.大豆加工与利用[M]. 北京：化学工业出版社，2003.(Li L T. Soybean processing and utilization[M].Beijing: Chemical Industry Press, 2003.) [2]Mujoo R,Trinh D T,Ng P K W. Characterization of storage proteins in dieffrent soybean varieries and their relationship to Tofu yield texture[J].Food Chemistry,2003，82:265-273. [3]Hau Yin Chung.Volatile flavor components in red fermented soybean(Glycine max)Curds[J].Journal Agricultural Food Chemistry,2000,48:1803-1809. [4]Wanakhachornkrai,P Lertsiri S. Comparison of determination method for volatile compounds in Thai soy sauce[J].Food Chemstry,2003,83:619-629. [5]毛礼钟，毛吴.大豆和豆制品的营养价值与豆腐的新品种[J].中国烹饪研究，1997(4):40-43.(Mao L Z,Mao W. The nutritional value of soybeans and soybean products and new varieties of Tofu[J]. Study of Chinese cuisine, 1997(4):40-43.) [6]杨 峥 ,李连生 ,邓 旭 ,等.酱油中香气成分的剖析[J].食品与发酵工业,1990(2):18-24.（Yang Z,Li L S,Deng X,et al. Analysis of aroma components in soy sauce[J]. Food and Fermentation Industry, 1990(2):18-24.） [7]王林祥 ,刘杨岷 ,王建新.酱油风味成分的分离鉴定[J].中国调味品,2005(1):45-48.(Wang L X,Liu Y M,Wang J X. Isolation and identification of flavor components of soy sauce[J]. Chinese Seasoning, 2005(1):45-48.) [8]吕健，阮晓明，盛志艺，等. 固相微萃取与同时蒸馏萃取法分析香精成分比较[J].烟草科技/烟草化学，2003(2)：52-56. （Lv J,Ruan X M,Sheng Z Y, et al. Comparison between Solid Phase Micro-extraction and Simultaneous Distillation and Extraction Methods for Analysis of Aroma Components in Tobacco Flavor[J]. Tobacco Science&Technology, 2003(2)：52-56.） [9]陈楚良. 固相微量萃取技术[J].上海环境科学，1997,16（4）:39-42.(Chen C L. Solid-phase micro-extraction technology[J]. Shanghai Environmental Science, 1997,16（4）:39-42.) [10]卞顺平，翁新楚.豆腐挥发性风味成分的研究[J].上海大学学报，2008，14(1):100-105.（Bian S P,Weng C X, Volatile flavor constituents of Tofu[J]. Journal of Shanghai University, 2008，14(1):100-105.）
备注/Memo
第一作者简介：李晶（1985-）,女，在读硕士，研究方向为食品生物技术。E-mail：lijing_1032@yahoo.com.cn。