[1]潘怡欧,秦正睿,席景会.大豆蚜玻璃管药膜法敏感毒力基线的建立[J].大豆科学,2010,29(03):483-485.[doi:10.11861/j.issn.1000-9841.2010.03.0483]
　PAN Yi-ou,QIN Zheng-rui,XI Jing-hui.Establishment of Susceptible Toxicity Baseline for Aphis gylcnies Matsumura by the Method of Residual Film in Glass Tube[J].Soybean Science,2010,29(03):483-485.[doi:10.11861/j.issn.1000-9841.2010.03.0483]
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大豆蚜玻璃管药膜法敏感毒力基线的建立

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年03期 页码: 483-485 栏目: 出版日期: 2010-06-25
Title:
Establishment of Susceptible Toxicity Baseline for Aphis gylcnies Matsumura by the Method of Residual Film in Glass Tube
文章编号:
1000-9841（2010）03-0483-03
作者:
潘怡欧; 秦正睿; 席景会
吉林大学 植物科学学院，吉林 长春 130062
Author(s):
PAN Yi-ou;  QIN Zheng-rui;  XI Jing-hui
College of Plant Science, Jilin University, Changchun 130062，Jilin，China
关键词:
大豆蚜; 玻璃管药膜法; 敏感毒力基线; 抗药性监测
Keywords:
Aphis gylcnies Matsumura; Residual film method; Susceptible toxicity baseline; Resistance monitoring
分类号:
S435.2
DOI:
10.11861/j.issn.1000-9841.2010.03.0483
文献标志码:
A
摘要:
以采自吉林长春地区田间的大豆蚜在室内不接触药剂饲养25代以上，利用玻璃管药膜法建立了大豆蚜对新烟碱类、氨基甲酸酯类、拟除虫菊酯类、有机磷类共10种杀虫剂3.5 h的敏感毒力基线。结果表明：大豆蚜对这10种杀虫剂的敏感度较高，LC50从小到大依次为：吡虫啉（0.105 μg·g-1）、功夫菊酯（0.924 μg·g-1）、溴氰菊酯（1.216 μg·g-1）、毒死蜱（1.557 μg·g-1）、灭多威（1.918 μg·g-1）、克百威（3.927 μg·g-1）、马拉硫磷（5.125 μg·g-1）、辛硫磷（12.551μg·g-1）、氧乐果（13.190 μg·g-1）、氰戊菊酯（28.569 μg·g-1）。所测得结果可作为敏感毒力基线，并为大豆蚜的抗药性监测提供理论依据。
Abstract:
The susceptible toxicity baselines was established by the method of the residual film in glass tube with 3.5 h exposure to the 10 kinds of insecticides including Neonicotinoid, Carbamate, Pyrethroid and Organophosphate for Aphis gylcnies Matsumura, which was collected from Changchun area. The results showed that Aphis gylcnies Matsumura was susceptible to the 10 kinds of insecticides，the LC50 of the insecticides tested was arranged in increasing order as Imidacloprid (0.105 μg·g-1), Cyhalothrin (0.924 μg·g-1), Deltamethrin (1.216μg·g-1), Chlorpyrifos(1.557μg·g-1), Methomyl(1.918μg·g-1), Carbosulfan(3.927μg·g-1), Malathion(5.125μg·g-1), Phoxim(12.551μg·g-1), Omethoate(13.190 μg·g-1), Fenvalerate(28.569 μg·g-1). The results of toxicity test provided a theoretical basis for resistance monitoring of soybean aphid.

参考文献/References:

[1]苗进，吴孔明，李国勋. 大豆蚜的研究进展[J]. 大豆科学，2005，24（2）：135-138.（Miao J, Wu K M, Li G X. Advances in research on soybean aphid, Aphis Glycines[J]. Soybean Science，2005，24（2）：135-138.）

[2]吴益东，陈松净，净新娟，等. 棉铃虫抗药性监测方法——浸叶法敏感毒力基线的建立及其应用[J].昆虫学报，2001，44(1): 56-61.（Wu Y D，Chen S，Jing X J，et al. Susceptible toxicity baseline and its application in resistance monitoring of Helicoverpa armigera?with leaf dipping method[J]. Acta Entomologica Sinica，2001，44(1): 56-61.）

[3]Shotkoski F A,Mayo Z B, Peters L L. Induced disulfoton resistance in greenbugs (Homoptera: Aphididae)[J].Journal of Economic Entomology, 1990, 83(6): 2147-2152.

[4]Shufran R A, Wilde G E, Sloderbeck P E. Response of three greenbug (Homoptera: Aphididae) strains to five organophosphorous and two carbamate insecticides[J]. Journal of Economic Entomology, 1997,90(1):283-286.

[5]Moores G D,Gao X W, Denholm I, et al. Characterization of insensitive acetylcholinesterase in the insecticide-resistant cotton aphid,Aphis gossypii Glover (Homoptera:Aphidedae)[J]. Pesticide Biochemistry and Physiology, 1996,56(2): 102-110.

[6]陈长琨，李秀峰，韩召军，等. 二化螟抗药性监测方法及相对敏感基线[J]. 南京农业大学学报，2000，23(4): 25-28. （Chen C K, Li X F, Han Z J, et al Method for monitoring insecticide resistance in rice stem borer (Chilo suppressalis?Walker) and relative susceptible baselines data[J]. Journal of Nanjing Agricultural University，2000，23(4): 25-28.）

[7]鲁艳辉，杨婷，高希武. 禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立[J]. 昆虫学报，2009，52(1):52-58. （Lu Y H，Yang T，Gao X W. Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphum padi (Linnaeus) and Sitobion avenae (Fabricius)(Homoptera: Aphididae) by the method of residual film in glass tube[J]. Acta Entomologica Sinica，2009，52(1):52-58.）


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备注/Memo

基金项目：吉林大学博士引进人才科研启动基金资助项目（4305050102H5）;吉林大学基本科研业务基金项目（421021276202）。

第一作者简介：潘怡欧（1978-），女，博士，讲师，研究方向为昆虫毒理学。E-mail：panyiou@gmail.com。
通讯作者：席景会，教授。 E-mail：jhxi1965@jlu.edu.cn。

更新日期/Last Update: 2014-09-14