WANG Yan,ZHAO Yi-xin,SUN Jia-wei,et al.Effects of Reactive Oxygen Species Metabolic System on Soybean (Glycine max) under Exogenous Chitosan to Ozone Stress[J].Soybean Science,2010,29(03):408-412.[doi:10.11861/j.issn.1000-9841.2010.03.0408]
结荚期喷施壳聚糖对臭氧胁迫下大豆活性氧代谢系统的影响
- Title:
- Effects of Reactive Oxygen Species Metabolic System on Soybean (Glycine max) under Exogenous Chitosan to Ozone Stress
- 文章编号:
- 1000-9841（2010）03-0408-05
- Keywords:
- Exogenous CTS; Ozone stress; ROS metabolic
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为揭示外源壳聚糖对O3胁迫下农作物活性氧代谢系统的影响机理，以大豆（Glycine max）为研究材料，利用开顶式气室（OTCs）研究了结荚期喷施CTS对O3胁迫下大豆叶片活性氧自由基产生速率、含量，膜脂过氧化程度，抗氧化酶活性，抗氧化物质含量及大豆籽粒产量的影响。结果表明：在整个生育期内，与对照相比，高浓度O3处理下，大豆叶片O2产生速率、H2O2含量升高，MDA含量、相对电导率增大，SOD、CAT、POD活性下降，ASA、GSH含量及大豆籽粒产量降低，对大豆表现为伤害效应。结荚期喷施外源CTS后，经O3胁迫的大豆叶片O2产生速率、H2O2含量降低，MDA含量、相对电导率减小，SOD、CAT、POD活性升高，ASA、GSH含量以及大豆籽粒产量增加，表明外源CTS一定程度上缓解了O3胁迫对大豆的伤害，但这种缓解作用是有限的。
- Abstract:
- In order to reveal effect mechanism of crop reactive oxygen species (ROS) metabolic system under exogenous chitosan (CTS) to ozone (O3) stress, open top chambers were utilized to investigate the change of ROS production rate, lipid peroxidation extent, anti-oxidative enzymes activities and antioxidant content in soybean (Glycine max) leaves and the grains yield.The results indicated that,compared to the control,the elevated O3 concentration induced an increase on O2production rate,H2O2content,MDA content and relative electrical conductivity and a decrease on the activities of SOD,CAT and POD,the content of AsA and GSH in the whole growth stage and also a decline on grains yield. Exogenous CTS treatment relieves the aggravation of ROS damage through ozone stress, which represents protective efficacy to soybean, O2production rate, H2O2content, MDA content and membrane permeability decreased, while anti-oxidative enzymes activity and anti-oxidative substances increased. But the alleviation of exogenous CTS to ozone is limited.
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备注/Memo
基金项目：国家自然科学基金资助项目（30970448，30500069）。