WU Li-fang,WU Ya-nan,WANG Hong-he,et al.Effects of Main Soybean Antigen on Nutritional Components in Muscle of Carp(Cyprinus carpio)[J].Soybean Science,2010,29(01):105-108.[doi:10.11861/j.issn.1000-9841.2010.01.0105]
大豆主要抗原蛋白对鲤鱼肌肉营养成分的影响
- Title:
- Effects of Main Soybean Antigen on Nutritional Components in Muscle of Carp(Cyprinus carpio)
- 文章编号:
- 1000-9841（2010）01-0105-04
- 分类号:
- S963.16
- 文献标志码:
- A
- 摘要:
- 以初始体质量（31.34±0.29）g的健康鲤鱼鱼种为试验对象，以鱼粉为对照组，β-伴大豆球蛋白的添加量为40 ·kg-1，大豆球蛋白的添加量为60 ·kg-1，各配制成3种等蛋白（360 ·kg-1）等能(15.2 M·kg-1)的半精制饲料，在室内单循环控温养殖系统中进行6周饲养试验，探讨大豆主要抗原蛋白（β-伴大豆球蛋白和大豆球蛋白）对鲤鱼肌肉主要营养成分的影响。结果表明：大豆主要抗原蛋白使鲤鱼肌肉中粗蛋白含量、必需氨基酸总量下降，但与对照组差异不显著（P＞0.05）；鲤鱼肌肉中粗脂肪、粗灰分、氨基酸总量、鲜味氨基酸总量与对照组差异不显著（P＞0.05）。因此，大豆主要抗原蛋白对鲤鱼肌肉营养成分尚未造成显著影响。
- Abstract:
- The research investigated the effects of main soybean antigens(glycinin or β-conglycinin)on nutritional components of muscle of Cyprinus carpio.A growth trial was conducted on Cyprinus carpio with the initial weight 31.34±0.29 g for 6 weeks at controlled temperature in single recirculating system.Fish meal used as animal protein and purified β-conglycinin(40 g?kg-1 diet)or glycinin(60 ·kg-1 diet)was conducted as three isonitrogenous(360 ·kg-1)and isoenergetic(15.2 M·kg-1)experimental diets，respectively.The results indicated that crude protein content，contents of total essential amino acids in muscle of Cyprinus carpio fed with β-conglycinin or glycinin declined，but there were no significant differences between treated groups and control group(P＞0.05).The contents of fat，ash content，total amino acids and total flavor amino acids in muscle were not significantly influenced（P＞0.05）.Therefore，main soybean antigens have no significant effects on the nutritional components in muscle of Cyprinus carpio.
参考文献/References:
[1]舒妙安，马有智，张建成.黄鳝肌肉营养成分的分析[J].水产学报，2000，24(4):339.(Shu M A，Ma Y Z，Zhang J C.An analysis of the nutritive composition in muscle of Monopterus albus[J].Journal of Fisheries of China，2000，24(4):339.) [2]王伟，陈立侨，顾志敏，等.六个群体翘嘴红鲌肌肉生化组成的比较[J].水产学报，2007，31(增刊):93-98.(Wang W，Chen L Q，Gu Z M，et al.Comparative study on muscle nutritional quality of six different geographical populations of Culter alburnus.[J].Journal of Fisheries of China，2007，31(Suppl):93-98.) [3]谭德清，王俭伟，但胜国.黑尾近红鲌含肉率及肌肉营养成分分析[J].水生生物学报，2004，28（3）:204-246.(Tan D Q，Wang J W，Dan S G.The ratio of flesh to body and analysis on nutritive composition of muscle in ancherythroculter nigrocauda[J].Acta Hydrobiologica Sinica，2004，28（3）:204-246.) [4]Catsimpoolas N，Ekenstam C.Isolation of α-，β-，and γ-conglycinins[J].Archives of Biochemistry and Biophysics，1969，129（2）:490-497. [5]D F Li ，Nelssen J L，Reddy P G，et al.Transicnt hypcrscnsitivity to soybean meal in the early weaned pig[J].Journal of Animal Sciebce，1990，68:1790-1799. [6]Seegraber F J，Morrill J L.Effect of protein source in calf milk replacers on morphology and absorptive ability of the small intestine[J].Journal of Dairy Science，1986，69(2):460-469. [7]Johnston C .Effect of injecting lambs with soyflour extract on serum soy protein antibody concentration and rate of gain[J].Small Ruminant Research，1996，21(2):149-154. [8]Christensen H R，Susanne W B，Frokiaer H.Antigenic specificity of serum antibodies in mice fed soy protein[J].International Archives of Allergy and Applied Immunology，2003，132(1):58-67. [9]Wu Shaowen，Murphy P A，Johnson L A，et al.Simplified process for soybean glycinin and β-conglycinin fractionation[J].Journal of Agricultural and Food Chemistry，2000，48:2702-2708. [10]陈乃松，艾庆辉，王道尊.欧洲鳗配合饲料中大豆蛋白替代鱼粉的研究[J].水产学报，1998，22（3）:283-287.(Chen N S，Ai Q H，Wang D Z.Studies on soybean protein as a substitute for fish meal in formulate diets for Anguilla anguilla[J].Journal of Fisheries of China，1998，22（3）:283-287.) [11]Refstie S，Storebakken T，Baeverfjord G，et al.Long-term protein and lipid growth of Atlantic salmon(Salmo salar)fed diets with partial replacement of fish meal by soy protein products at medium or high lipid levels[J].Aquaculture，2001，193:91-106. [12]Chou R L ，Her B Y ，Su M S ，et al.Substituting fish meal with soybeanmeal in diets of juvenile cobia Rachycentron canadum[J].Aquaculture，2004，229:325-333.
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备注/Memo
基金项目：国家自然科学基金资助项目（30430520）。