HE Shi-min,XIANG Deng-yun,YIN Shi-shi.Investigation of the Urea Enzyme Catalysis Specific Property in the Sprouting Period of Soybean[J].Soybean Science,2009,28(05):856-858.[doi:10.11861/j.issn.1000-9841.2009.05.0856]
大豆种子萌发期脲酶催化特性的研究
- Title:
- Investigation of the Urea Enzyme Catalysis Specific Property in the Sprouting Period of Soybean
- 文章编号:
- 1000-9841(2009)05-0856-03
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 根据脲酶水解尿素后释放NH3而使得水溶液pH值升高的原理，通过冷冻离心提取酶液和酸度计测定△pH的方法，测知大豆种子萌发期脲酶的活性，为大豆的合理栽培提供参考。结果表明：合丰25大豆，在底物浓度为0.25 mol.L-1时，脲酶活性最强；辽豆7号，在底物浓度为0.35 mol.L-1时，脲酶活性最强；2个大豆脲酶的最适温度是70℃；大豆种子水培养24 h，脲酶活性最强。合丰25的尿酶活性大于辽豆7号。大豆；脲酶；催化特性；底物浓度；温度
- Abstract:
- Urea enzyme influence environmental urea utilization ratio directly during sprouting of soybean seeds.According to the principle that urea will release NH3 when hydrolyzed by urea enzyme and hence increase pH of aqueous solution.Soybean seeds of Hefeng 25 and Liaodou 7 were soaked in distilled water for 12 h，and then cultured in incubator at 25℃ for 0，24，36，48 and 72 h，respectively.Then the cultured soybean seeds were smashed and centrifuge to obtain the urea enzyme sample solution.The activity of urea enzyme under different concentration of urea phosphate buffer and temperature were calculated based on the △pH.Results showed that the urea enzymic activity of Hefeng 25 was maximum when concentration of substrate was 0.25 mol.L-1；while for Liaodou 7 the concentration of substrate was 0.35 mol.L-1.The most suitable temperature for urea enzymic activity was 70℃，and soybean seeds had highest urea enzyme activity when cultured for 24 h.The Urea enzyme activity of Hefeng 25 was higher than that of Liaodou 7.
参考文献/References:
[1]张龙翔，张庭芳，李令媛.生化实验方法和技术[M].北京：高等教育出社，1981:145-149.（Zhang L X，Zhang T F，Li L Y.Method and technology of biochemical experiment[M].Beijing：Higher Education Press，1981，145-149.）
备注/Memo
基金项目：长江师范学院重点扶持学科资助项目。