[1]刘顺湖,周瑞宝,盖钧镒.中国野生和栽培大豆11S及7S蛋白质相对含量的比较分析[J].大豆科学,2009,28(05):759-757.[doi:10.11861/j.issn.1000-9841.2009.05.0759]
　LIUShun-hu,ZHOU Rui-bao,GAIJun-yi.Comparative Analysis of 11S and 7S Protein Relative Content between Wild and Cultivated Soybeans in China[J].Soybean Science,2009,28(05):759-757.[doi:10.11861/j.issn.1000-9841.2009.05.0759]
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中国野生和栽培大豆11S及7S蛋白质相对含量的比较分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 759-757 栏目: 出版日期: 2009-10-25
Title:
Comparative Analysis of 11S and 7S Protein Relative Content between Wild and Cultivated Soybeans in China
文章编号:
1000-9841(2009)05-0759-09
作者:
刘顺湖1; 3; 周瑞宝2; 盖钧镒1
1.南京农业大学 大豆研究所，国家大豆改良中心，作物遗传与种质创新国家重点实验室,江苏 南京 210095；
2.河南工业大学 大豆精深加工研究所，河南 郑州 450052；
3.济宁学院，山东 曲阜 273155
Author(s):
LIUShun-hu1; ZHOU Rui-bao2; GAIJun-yi1
1.Soybean Research Institute of Nanjing Agricultural University,National Center for Soybean Improvement,National Key Laboratory for Crop Genetics and Germplasm Enhancement，Nanjing 210095，Jiangsu；
2.Soybean Processing Research Institute，Henan University of Technology，Zhengzhou 450012，Henan；
3.Jining College，Qufu 273155，Shandong，China
关键词:
野生大豆(G.soja Sieb.et Zucc.); 栽培大豆(G.max(L.）Merr.); 11S蛋白质; 7S蛋白质; 11S/7S比值; 蛋白质亚基组; 遗传变异; 生态区
Keywords:
Wild soybean（G.soja Sieb.et Zucc.); Cultivated soybean（G.max(L.）Merr.); 11S protein; 7S protein; 11S/7S ratio; Protein subunit group; Genetic variability; Eco-region
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0759
文献标志码:
A
摘要:
大豆种质蛋白质11S和7S及其亚基组相对含量的遗传变异是专用型品种选育的基础。以全国各生态区的野生豆138份和地方品种409份，国内育成品种148份、国外育成品种83份，合计778份大豆种质为材料，采用SDS-PAGE电泳技术测定蛋白质11S和7S组分及其亚基组相对含量，研究其遗传变异。在南京同一条件下的结果表明：全国野生豆、地方品种和育成品种11S相对含量平均分别为54.7%、64.8%和71.7%，变幅28.8%~82.6%、38.8%~79.4%和48.2%~88.9%；7S相对含量平均分别为44.7%、34.9%和27.9%，变幅20.6%~71.2%、20.6%~61.1%和15.7%~47.8%；11S/7S比值平均分别为1.4、2.0和2.7，变幅0.4~3.9、0.6~3.9和0.9~4.0。野生豆驯化为栽培豆并经选育后11S相对含量和11S/7S比值上升，7S相对含量下降，变幅均减小；亚基组11S-2和11S-3相对含量增加；7S的6个亚基组，尤其7S-1和7S-6，相对含量下降。11S、7S、11S/7S以及各亚基组在各群体各生态区内均有较大变异，但与来源地纬度、蛋白质和油脂含量均无显著相关。从中优选到11S/7S比值大于3.7、11S相对含量为78.9%~88.9%的8份种质，发现有11S的4个亚基组相对含量分别大于37%、7S的6个亚基组相对含量分别大于24%、以及11S-1和7S的6个亚基组缺失的种质，这些特异种质可供蛋白质组分育种利用。
Abstract:
In breeding for cultivars with specific protein quality，it should be the basic step to hold the genetic variability of relative content of 11S，7S protein and their subunit groups in soybean germplasm.In the present study，total 778 accessions，including 138 wild entries（Glycine soja Sieb.et Zucc.)，409 cultivated landraces（Glycine max（L.）Merr.)and 148 released domestic cultivars sampled from various eco-regions as a representative in China，along with 83 released foreign cultivars,were tested for their 11S，7S protein and their subunit group relative contents by using SDS-PAGE analysis.The obtained data were analyzed with the software of SAS 9.0 and SPSS 13.0.The results obtaind under environment conditions in Nanjing are as follows:The mean relative content of 11S in wild accessions，landraces and released cultivars was 54.7%，64.8% and 71.7% with a range of 28.8%-82.6%，38.8%-79.4% and 48.8%-88.9%，respectively.That of 7S was 44.7%，34.9% and 27.9% with a range of 20.6%-71.2%，20.6%-61.1% and 15.7%-47.8%，respectively.That of 11S/7S ratio was 1.4，2.0 and 2.7 with a range of 0.4-3.9，0.6-3.9 and 0.9-4.0，respectively.Due to domestication and artificial breeding，the 11S relative content and 11S/7S ratio increased and the 7S relative content decreased gradually with their ranges all decreased，while the relative contents of subunit group 11S-2 and 11S-3 increased and those of the six 7S subunit groups，especially 7S-1 and 7S-6 decreased.There existed abundant variation in 11S and 7S relative content，11S/7S ratio and their subunit groups in the three germplasm types in each eco-region，but no significant correlation with geographic latitude of accession’s source site，as well as with protein and fat content was found.Eight elite accessions were screened out from the 778 accessions to be with 11S/7S ratio more than 3.7 and 11S relative content 78.9%-88.9%.In addition，there found also the accessions with 11S subunit group relative content more than 24% and the accessions with 11S-1 deficient or one or two 7S subunit groups deficient.These materials should be the superior sources for breeding programs in protein quality improvement.

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备注/Memo

基金项目：国家重点基础研究发展规划资助项目(2004CB7206，2006CB101708，2009CB118404)；国家自然科学基金资助项目(30671266)；国家高新技术研究发展计划资助项目(2006AA100104)；教育部高等学校创新引智计划资助项目(B08025)；农业部公益性行业专项（200803060）。

作者简介：刘顺湖（1960-），男，副教授，博士研究生，从事大豆遗传与育种研究与教学。
通讯作者：盖钧镒，教授，中国工程院院士。 E-mail：sri@njau.edu.cn；周瑞宝，教授。E-mail：rbzhou0615@163.com。南京农业大学和河南工业大学均为第一完成单位。

更新日期/Last Update: 2014-09-13