[1]宋晓慧,李冬梅,段莹莹,等.体细胞胚诱导条件优化及杂交后代再生遗传分析[J].大豆科学,2008,27(05):751-754,759.[doi:10.11861/j.issn.1000-9841.2008.05.0751]
　SONG Xiao-hui,LI Dong-mei,DUAN Ying-ying,et al.Optimization of Somatic Embryo Induction and Genetic Analysis of Offspring[J].Soybean Science,2008,27(05):751-754,759.[doi:10.11861/j.issn.1000-9841.2008.05.0751]
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体细胞胚诱导条件优化及杂交后代再生遗传分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年05期 页码: 751-754,759 栏目: 出版日期: 2008-10-25
Title:
Optimization of Somatic Embryo Induction and Genetic Analysis of Offspring
文章编号:
1000-9841(2008)05-0751-04
作者:
宋晓慧1; 李冬梅1; 段莹莹1 ; 韩英鹏1; 李春光2 李文滨1
1东北农业大学大豆研究所,大豆生物学教育部重点实验室，黑龙江 哈尔滨 150030;
2黑龙江省农垦科学院水稻研究所，黑龙江佳木斯 154025
Author(s):
SONG Xiao-hui1; LI Dong-mei1; DUAN Ying-ying1; HAN Ying-peng1; LI Chun-guang2; LI Wen-bin1
1Soybean Research Institute,Key Laboratory of Soybean Biology of Chinese Education Ministry，Northeast Agricultural University，Harbin 150030;
2Rice Research Institute of Land Reclamation Academy of Heilongjiang Province，Jiamusi 154025，Heilongjiang,China
关键词:
大豆再生; 未成熟子叶; 体细胞胚诱导; 遗传分析
Keywords:
Soybean regeneration; Immature cotyledon; Somatic embryogenesis induction;  Genetic ananlysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.05.0751
文献标志码:
A
摘要:
以北京小黑豆未成熟子叶为外植体,针对影响体细胞胚发生的几个关键因素进行研究。结果表明：pH等于7，外植体大小为2~3 mm和暗培养条件最适合体细胞胚的发生。利用优化后的再生系统诱导了北京小黑豆（再生性强）和Keburi（再生性极低）杂交所衍生的150个F5：6重组自交系，体细胞胚发生频率明显呈现间断分布，由此初步推断大豆体细胞发生频率由少数几个基因控制。由体细胞胚发生效率分布偏向Keburi，可见Keburi对体细胞胚发生效率影响要比北京小黑豆大。
Abstract:
With Peking immature cotyledon as explants，key factors that affecting somatic embryogenesis were evaluated.The results showed that pH = 7.0，explant size of 2-3 mm and dark culture conditions were beneficial for the occurrence of somatic embryogenesis，which was proved efficient for somatic embryogenesis.One hundred and fifty F5：6recombinant inbred lines derived from Peking×Keburi was cultured with inducing medium for embryogenesis.The results showed that frequency distribution was discrete，suggesting that embryogenesis was controlled by few genes.Meanwhile，the impact of Keburi on embryogenesis efficiency was higher that that of Peking.

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备注/Memo

基金项目：黑龙江省攻关课题资助项目（GA06B103-9）。

作者简介：宋晓慧（1978-），女，硕士研究生，研究方向大豆生物技术。E-mail:songxiaohui20070508@yahoo.cn。
通讯作者：李文滨，教授, 博士生导师。E-mail:wenbinli@yahoo.com。

更新日期/Last Update: 2014-10-06