[1]周三,关崎春雄,岳旺,等.野生大豆、黑豆和大豆的异黄酮类成分比较[J].大豆科学,2008,27(02):315-319.[doi:10.11861/j.issn.1000-9841.2008.02.0315]
　ZHOU San,SEKIZAKI Haruo,YUE Wang,et al.Comparison of Isoflavones among Wild，Black and Yellow Soybeans[J].Soybean Science,2008,27(02):315-319.[doi:10.11861/j.issn.1000-9841.2008.02.0315]
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野生大豆、黑豆和大豆的异黄酮类成分比较

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 315-319 栏目: 出版日期: 2008-04-30
Title:
Comparison of Isoflavones among Wild，Black and Yellow Soybeans
文章编号:
1000-9841(2008)02-0315-05
作者:
周三; 关崎春雄2; 岳旺1; 泽聪子2; 杨志宏1
1青岛大学医学院药学系，山东 青岛 266021；
2 北海道医疗大学药学部，日本 北海道 061-0293
Author(s):
ZHOU San1; SEKIZAKI Haruo2; YUE Wang 1; SAWA Satoko 2; YANG Zhi-hong 1
1 Pharmic Department，Medical Collage，Qingdao University，Qingdao 266021，Shangdong，China；
2 Faculty of Pharmaceutical Sciences，Health Sciences University of Hokkaido，Hokkaido 061-0293，Japan
关键词:
野生大豆; 黑豆; 大豆; 大豆异黄酮
Keywords:
Wild soybean; Black soybean; Yellow soybean; Soybean isoflavones
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0315
文献标志码:
A
摘要:
野生大豆主产于中国，古时为珍贵药材，但现代将其作药用的研究较少。以同样种植条件下收获的野生大豆、黑豆和大豆为材料，用HPLC法、凯氏定氮法和索氏残渣法分别测定其大豆异黄酮、蛋白质和脂肪含量，以此比较野生大豆、黑豆和大豆的药用价值。结果表明：12种大豆异黄酮类组分的总含量大小顺序为：野生大豆>黑豆>大豆；野生大豆和黑豆中黄豆苷和染料木苷含量特别突出，而且其相应的苷元含量也较高，大豆中则是丙二酰基化的黄豆苷和丙二酰基化的染料木苷含量特别突出，而其相应的苷元组分含量极低；相关性分析表明三种大豆的不同品种，大豆异黄酮总含量与其百粒重和脂肪含量呈极显著负相关，但与蛋白质含量的相关性不显著。结果说明野生大豆的药用研究应受到重视。
Abstract:
The wild soybean(Glycine soja) mainly distributes in China，and it is used as a precious medicinal material in ancient times，but its medicinal application is nearly neglected in modern time.To compare the medical value among wild，black and yellow soybeans，different varieties of wild，black and yellow soybeans were cultivated under same conditions，and isoflavones，protein and lipoid content were determined with HPLC，Kieldahl method and Soxhlet residual method，respectively.The results showed the total isoflavones content was in the order of wild > black > yellow soybeans；the contents of daidzin and genistin were much higher than other isoflavone components in wild and black soybeans，while the contents of 6″-o-malonyldaidzin，6″-o-malonylgenistin were much higher in yellow soybeans；the contents of daidzein and genistein were relatively higher in wild and black soybeans than in yellow soybeans.By correlation analysis，in the different varieties of wild，black and yellow soybeans，the total isoflavones content was negatively significantly correlated with 100-seed weight and lipoid content，while its correlation with protein content was not significant.So it is suggested that the wild soybean should be thought highly as an important medicinal material.

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备注/Memo

基金项目：青岛市科技发展计划资助项目(04-2-NY-47)；山东省优秀中青年科学家科研奖励基金资助项目(2005BS08011)。

作者简介：周三 (1966-)，男，理学博士，副教授，硕士生导师，现从事植物化学领域的研究。E-mail: zhousan3@yahoo.com.cn。

更新日期/Last Update: 2014-10-10