[1]赵卫星,孙治强,高玉红,等.大豆荚壳中类黄酮提取工艺及其抑杀线虫活性的研究[J].大豆科学,2007,26(03):373-376.[doi:10.3969/j.issn.1000-9841.2007.03.018]
　ZHAO Wei-xing,SUN Zhi-qiang,GAO Yu-hong,et al.STUDIES ON THE EXTRACTION TECHNOLOGICAL CONDITIONS OF TOTAL FLAVONOIDS IN SOYBEAN HULL AND ITS NEMATICIDAL ACTIVITY[J].Soybean Science,2007,26(03):373-376.[doi:10.3969/j.issn.1000-9841.2007.03.018]
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大豆荚壳中类黄酮提取工艺及其抑杀线虫活性的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年03期 页码: 373-376 栏目: 出版日期: 2007-06-25
Title:
STUDIES ON THE EXTRACTION TECHNOLOGICAL CONDITIONS OF TOTAL FLAVONOIDS IN SOYBEAN HULL AND ITS NEMATICIDAL ACTIVITY
文章编号:
1000-9841(2007)03-0373-04
作者:
赵卫星1; 孙治强1 ; 高玉红2; 张楠1
1.河南农业大学林学园艺学院，郑州 450002；?
2郑州职业技术学院，郑州 450121
Author(s):
ZHAO Wei-xing1; SUN Zhi-qiang1; GAO Yu-hong2; ZHANG Nan1
1College of Forestry and Horticulture，Henan Agriculture University；Zhengzhou 450002；
2Zhengzhou Vocational and Technological College，Zhengzhou 450121
关键词:
大豆荚壳; 类黄酮; 提取工艺; 杀线虫活性
Keywords:
Soybean hull; Total flavonoids; Extracting technological conditions; Nematicidal activity
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.03.018
文献标志码:
A
摘要:
阐述从大豆荚壳中提取类黄酮的工艺及其抑杀根结线虫的应用效果。通过正交设计，确定了大豆类黄酮的最佳提取工艺条件为乙醇浓度80％，提取温度70℃,乙醇与大豆荚壳1：10，回流提取24 h,提取率可达8.58%，显著高于次佳工艺。对根结线虫抑杀效果的测定表明：类黄酮粗提物对南方根结线虫J2有很强的毒杀效果，且随使用浓度增大而增强，1.5 μgmg-1类黄酮提取物处理，线虫的校正死亡率可达50%以上；对虫卵孵化有一定的抑制作用，但孵化抑制率都在50%以下。
Abstract:
The optimal extracting technological conditions of flavonoids from soybean hull by orthogonal plan method and its nematicidal activity were studied.The result indicated that the conditions of 70℃,10:1 of 80% ethanol to soybean hull,heating and refluxing for 24 h were optimium，the extractive ratio could be up to 8.58% and was higher than Hypo-optimal conditions significantly；The total flavonoids of the extractive solution had high nematicidal activity against the second juvenile(J2),and with the in creasing of concentration，the nematicidal activity was improved，more than 50% of J2 could be killed with the treatment of 1.5 μgmg-1total flavonoids extraction.In addition,it also had inhibition on eggs hatch to some extent,but inhibition rate of eggs hatch was below 50%

参考文献/References:

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备注/Memo

基金项目：河南省重大科技攻关项目（0322010900）

作者简介：赵卫星（1978－），在读博士，研究方向设施园艺及蔬菜无公害生产。

更新日期/Last Update: 2014-10-22