[1]陶红　梁歧.酶法制备大豆寡肽的研究[J].大豆科学,2004,23(01):26-29.[doi:10.11861/j.issn.1000-9841.2004.01.0026]
　Tao Hong Liang Qi.STUDY ON PREPARATION OF SOYBEAN OLIGO-PEPTIDE BY ENZYME[J].Soybean Science,2004,23(01):26-29.[doi:10.11861/j.issn.1000-9841.2004.01.0026]
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酶法制备大豆寡肽的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷 期数: 2004年01期 页码: 26-29 栏目: 出版日期: 2004-02-25
Title:
STUDY ON PREPARATION OF SOYBEAN OLIGO-PEPTIDE BY ENZYME
文章编号:
1000 -9841(2004)01 -0026 -04
作者:
陶红　梁歧
解放军军需大学军需工程系, 长春130062
Author(s):
Tao Hong 　Liang Qi
The Quatermaster Universi ty of PLA , Changchun 130062
关键词:
大豆寡肽; 酶水解; 分子量; DH
Keywords:
Soybean oligo -peptide ; Enzymatic hydroly sis ; Molecular w eight ; The deg ree of hy droly sis
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.01.0026
文献标志码:
A
摘要:
以Promatex 为工具酶对大豆分离蛋白进行水解, 制备大豆寡肽。分别讨论了热处理温度和时间对DH 值的影响, 确定了热处理条件为90 ℃、10min 。Promatex 的水解条件为:pH 值为7 .0 , 酶用量为0 .53ml , 酶添加量为E/S :10 .6 %, 温度50 ℃, 水解6hr 。制取的大豆肽的DH 为28 .23 %,PCL 在2 -4 之间为寡肽。
Abstract:
In this article the oligo -peptide was made by hydrolysis of soybean pro tein w ith promatex proteaseas tool enzyme .The ef fect of the tempratures and time of heating t reatment on DH of the hy drolysis of soybeanprotein w as studied respectively .The condi tion fo r heating treatment w as 90 ℃ fo r 15minutes .The optimumconditions for hy droly sis of soybean protein wi th promates is pH7 .0 , temperature :50 ℃, substrate concent ration :5 %, enzyme dosage :0 .53ml , reaction time :6hr .DH of soy bean peptide prepaired can be up to 28 .23 % at theoptimium conditions , and PCL of it is between 2 and 4 .It is the oligo -peptide .

参考文献/References:

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备注/Memo

作者简介:陶红(1971 -), 女, 讲师, 硕士, 研究方向大豆食品研究。

更新日期/Last Update: 2016-12-31