BI Wei-wei,ZHAO Gui-xing,XIA Xiao-yu,et al.Comprehensive Analysis on Soybean Sprout Characteristics of Main Soybean Varieties in Heilongjiang Province[J].Soybean Science,2021,40(02):209-216.[doi:10.11861/j.issn.1000-9841.2021.02.0209]
黑龙江省主栽大豆品种豆芽品质特性的综合分析
- Title:
- Comprehensive Analysis on Soybean Sprout Characteristics of Main Soybean Varieties in Heilongjiang Province
- Keywords:
- Soybean isoflavones; Germination time; Germination rate; Main cultivation; Sensory evaluation
- 文献标志码:
- A
- 摘要:
- 为筛选出黑龙江省适合的芽用大豆品种,提升豆芽附加值和开发潜力,本研究选取了黑龙江省11个主栽大豆品种,利用人工气候箱进行发芽试验,比较不同品种籽粒蛋白质和脂肪含量、发芽率、豆芽产率、 感官评定和异黄酮含量等指标,并利用隶属函数对豆芽品质特性进行了综合评价。结果表明:11个大豆品种蛋白、脂肪含量存在一定差异,单从发芽率和产率2个指标来看,适合生产豆芽的品种为黑农48、黑农66和黑农70。大豆发芽后,异黄酮总含量较籽粒显著增高,且在发芽第3天达到最高,显著高于大豆籽粒和其他发芽时间,异黄酮总含量增加最多的品种为黑农61和黑农63,分别是原来的1.63和1.76倍,黑农52的异黄酮含量第3天达到最高值,为636.38 mg?100 g-1。感官评定结果筛选出的品质较好品种为黑农59、黑农63和黑农70。通过发芽率、豆芽产率、感官评定和异黄酮含量4个指标的隶属函数综合评定,筛选出适合豆芽生产且异黄酮含量高的品种为黑农70。
- Abstract:
- In order to screen out suitable soybean varieties for sprouting in Heilongjiang Province, and improve the added value and development potential of soybean sprouts, 11 main soybean varieties in Heilongjiang Province were selected as materials in this study. The germination experiments were carried out in artificial climate box, and compared the protein and fat content, germination rate, soybean sprout yield, germination rate, sensory evaluation and isoflavones. The quality characteristics of bean sprouts were evaluated by membership function. The results showed that there were some differences in protein and fat contents among the 11 soybean varieties. From the germination rate and yield, the suitable varieties for bean sprout production were Heinong 48, Heinong 66 and Heinong 70. The content of isoflavones increased significantly after germination, and reached the highest on the third day of germination, which was significantly higher than that of soybean seeds and other germination stages. The total isoflavone content of Heinong 61 and Heinong 63 on the third day increased by 1.63 and 1.76 times to CK, respectively. The isoflavone content of Heinong 52 reached the highest value of 636.38 mg?100 g-1 on the third day. The results of sensory evaluation showed that the varieties with good quality were Heinong 59, Heinong 63 and Heinong 70. Through the comprehensive evaluation of the membership function of germination rate, bean sprout yield, sensory evaluation and isoflavone, the suitable soybean sprout variety with high isoflavone content was screened out as Heinong 70.
参考文献/References:
[1]黄明伟,于寒松,刘瑞雪,等.中国北方地区大豆主栽品种五种成分检测与分析[J].中国食物与营养,2015,21(8):31-34.(Huang M W, Yu H S, Liu R X, et al. Detection and analysis of five components of soybean cultivars in northern China[J]. Food and Nutrition in China,2015,21(8):31-34.)[2]张海军,王英,苏连泰,等.东北地区栽培大豆品种籽粒异黄酮含量分析及不同测定方法优化比较[J].大豆科学,2011,30(6):979-986.(Zhang H J, Wang Y, Su L T, et al. Analysis of isoflavone content in cultivated soybeans[Glycine max(L.)Merr.] in northeast China and comparison of different determination methods[J]. Soybean Science, 2011,30(6):979-986.)[3]于立梅,钟惠曾,于新,等. 大豆发芽过程中营养成分变化规律的研究[J]. 中国粮油学报,2010,25(8):19-22.(Yu L M, Zhong H Z, Yu X, et al. Change of nutritional composition during soybean germination[J]. Journal of the Chinese Cereals and Oils Association, 2010,25(8):19-22.)[4]王莘,胡可心,汪树生,等. 豆类萌发期蛋白质和氨基酸含量的比较分析[J]. 吉林农业大学学报,2003,25(1):21-23.(Wang S, Hu K X, Wang S S, et al. The comparison and analysis of protein and amino acid contents of beans in sprouting period[J]. Journal of Jilin Agricultural University, 2003,25(1):21-23.)[5]金庆跃,宋爱清,毛唯叶,等. 大豆异黄酮对不同雌激素受体基因型绝经后妇女骨代谢的影响[J]. 中国老年学杂志,2011,31(2):503-504.(Jin Q Y, Song A Q, Mao W Y, et al. Effects of soybean isoflavones on bone metabolism in postmenopausal women with different estrogen receptor genotypes[J]. Chinese Journal of Gerontology, 2011,31(2):503-504.)[6]韩小存,丁长河. 大豆膳食纤维和大豆异黄酮对糖尿病影响[J].粮食与油脂,2012(6):7-9.(Han X C, Ding C H. The effect of soybean dietary fiber and soybean isoflavones on diabetes[J]. Cereals & Oils, 2012(6):7-9.)[7]Hagen M K,Ludke A,Araujo A S,et al. Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model[J]. Canadian Journal of Physiology and Pharmacology,2012,90(8):1095-1103.[8]Yang Q,Qin L,Trust Beta. Antioxidant properties of commercial wild rice and analysis of soluble and insoluble phenolic acids[J]. Journal of Food Chemistry,2010,121:140-147.[9]陈玥,陈野.黄豆芽生产工艺及营养物质变化研究[J].食品研究与开发,2015,36(24):111-115, 137.(Chen Y, Chen Y. Studies on soybean sprouts production technology and nutrition changes[J]. Food Research and Development, 2015,36(24):111-115,137.)[10]线郁,张林,赵楠楠,等.优质豆芽菜生产研究现状与展望[J].安徽农业科学,2009,37(20):9446-9448.(Xian Y, Zhang L, Zhao N N, et al. Study advances and prospect of high-quality bean sprouts[J]. Journal of Anhui Agricultural Sciences, 2009,37(20):9446-9448.)[11]李晓红,李淑荣,罗红霞,等.芽苗活性成分研究进展[J].粮食与油脂,2019,32(12):1-4.(Li X H, Li S R, Luo H X, et al. A review of bioactive compounds in sprouts[J]. Cereals & Oils, 2019,32(12):1-4.)[12]张永清,顾振新,张颖,等.豆芽生产中大豆浸泡条件与吸水率和发芽率的关系研究[J].食品研究与开发,2007(11):26-29.(Zhang Y Q, Gu Z X, Zhang Y, et al. Study on sucked water rate and sprout rate associated with soaking condition in soybean sprout production[J]. Food Research and Development, 2007(11):26-29.)[13]高亮,孙继发,潘玲.酵素对绿豆芽生长发育、产量和品质的影响[J].蔬菜,2020(4):15-20.(Gao L, Sun J F, Pan L. Effects of enzyme on the growth, yield and quality of mungbean sprouts[J]. Vegetables, 2020(4):15-20.)[14]李长凤,王沙沙,崔小利,等.4-氯苯氧乙酸钠对大豆芽品质的影响及其安全风险性分析[J].食品与发酵工业,2017,43(10):80-87.(Li Y F, Wang S S, Cui X L, et al. Analysis of sodium 4- chlorophenxyacetate on bean sprouts quality and safety evaluation[J]. Food and Fermentation Industries, 2017,43(10):80-87.)[15]李振艳,张永忠,任红波. 大豆发芽过程中异黄酮、 γ-氨基丁酸等成分含量变化的研究[J]. 食品工业科技,2009,12:356-361.(Li Z Y, Zhang Y Z, Ren H B. Study on change of isoflavones, γ-GABA and other compositions during the germination of soybean[J]. Science and Technology of Food Industry, 2009,12:356-361.)[16]申海进,汪海峰,袁健,等. 大豆发芽期异黄酮含量变化的研究及其促进剂的影响[J]. 中国粮油学报,2012,21(6):75-77.(Shen H J, Wang H F, Yuan J, et al. Effect of accelerant on isoflavone content in soybean sprouts[J]. Journal of the Chinese Cereals and Oils Association, 2012,21(6):75-77.)[17]都启晶,王艺颖,丁君,等. 大豆发芽过程中维生素C、异黄酮含量变化及其抗氧化性的研究[J]. 食品安全质量检测学报,2015,6(9):3608-3613.(Du Q J, Wang Y Y, Ding J, et al. Changes of vitamin C, isoflavone and antioxidant activity during the germination of soybean[J]. Food Safety and Quality Detection Technology, 2015,6(9):3608-3613.)[18]曾庆真,牛文慧,邵淑娟.大豆发芽过程中异黄酮含量和抗氧化活性变化研究[J]. 粮食与油脂,2014,27(8):53-55.(Zeng Q Z, Niu W H, Shao S J. Study on change of isoflavone content and antioxidant activity during the germination of soybean[J]. Cereals & Oils, 2014,27(8):53-55.)[19]刘琴,牛文慧,张薇娜,等. 大豆与大豆芽中异黄酮的含量、组成及分布比较研究[J]. 食品工业科技,2013,21:60-64.(Liu Q, Niu W H, Zhang W N, et al. Comparison of the isoflavone content and isoflavone profile in soybean and germinated soybean[J]. Science and Technology of Food Industry, 2013,21:60-64.)[20]Luz M P,Mark A B,José Marcos G M. Effect of time and temperature on bioactive compounds in germinated Brazilian soybean cultivar BRS 258[J]. Journal of Food Research International,2010,43:1856-1865.[21]Guajardo-Flores D,García-Patio M,Serna-Guerrero D. Characterization and quantification of saponins and flavonoids in sprouts,seed coats and cotyledons of germinated black beans[J].Journal of Food Chemistry,2012,134:1312-1319.[22]丁杰. 改性乳清蛋白制备脂肪替代品的研究[D].天津:天津科技大学,2011.(Ding J. Study on the preparation of fat substitutes by modified whey protein[D]. Tianjin:Tianjin University of Science and Technology,2011.)[23]康玉凡,刘腾飞,程须珍,等.芽用绿豆品种子粒性状及其豆芽生理特性研究[J].植物遗传资源学报, 2011,12(6): 986-991.(Kang Y F, Liu T F, Chen X Z, et al. seed coat trait and physiological characteristics of sprout mung-bean[J]. Journal of Plant Genetic Resources, 2011,12(6): 986-991.)[24]尹成杰,王培,周玉丽,等. 8个豆芽豆品种籽粒性状及豆芽营养品质比较[J].安徽科技学院学报,2018,32(6):48-52.(Yi C J, Wang P, Zhou Y L, et al. Comparison of grain character and nutritional quality of bean sprout of eight soybean varieties[J]. Journal of Anhui Science and Technology University,2018,32(6):48-52.)[25]Park N I, Xu H, Li X, et al. Overexpression of phenylalanine ammonia-lyase improves flavones production in transgenic hairy root cultures of Scutellaria baicalensis[J]. Process Biochemistry, 2012,47(12): 2575-2580.[26]王慧芳,来吉祥,王东晖,等.中黄13大豆发芽期间异黄酮类成分变化规律的研究[J].中国粮油学报,2015,30(9):13-17.(Wang H F, Lai J X, Wang D H, et al. Kinetic changes of isoflavone components during germination in Zhonghuang 13[J]. Journal of the Chinese Cereals and Oils Association, 2015,30(9):13-17.)
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备注/Memo
收稿日期:2020-11-19