LIU Jia-ling,ZHANG Jun,HAN Xiao,et al.Protein and Fat Content QTL Mapping and Gene Annotation Based on High-density Soybean SNP Genetic Map[J].Soybean Science,2021,40(02):149-152.[doi:10.11861/j.issn.1000-9841.2021.02.0149]
基于高密度大豆SNP遗传图谱的蛋白质和脂肪含量QTL定位及基因注释
- Title:
- Protein and Fat Content QTL Mapping and Gene Annotation Based on High-density Soybean SNP Genetic Map
- Keywords:
- SNP; Protein; Fat; Genetic map; QTL mapping; Go annotation
- 文献标志码:
- A
- 摘要:
- ：为构建脂肪和蛋白含量上存在显著差异的大豆高密度遗传图谱，并对其进行基于SNP标记的QTL定位及定位区间内的基因注释，本研究以吉农45为母本、绥农76为父本杂交获得的F2代分离群体为基础，通过靶向测序基因型分型技术获得SNP标记，构建高密度遗传图谱，结合群体籽粒脂肪和蛋白含量，采用复合区间作图法（CIM）和完备区间作图法（ICIM）进行QTL定位，并根据SNP标记提供的染色体物理位置信息确定QTL所在的物理区间，利用Soybase和Phytozome数据库对区间进行基因挖掘、注释和筛选。结果表明：共获得2 399个多态性分子标记，构建了总图距为1 020.5 cM、标记间平均距离为0.61 cM 的遗传图谱。CIM法共检测到4个QTL，其中2个蛋白质含量位点分别为qPro-11-1和qPro-11-2，2个脂肪含量位点分别为qOil-7-1和qOil-19-1，贡献率为1.31%~21.69%。ICIM 法共检测到3个QTL，其中2个蛋白质含量位点分别为qPro-11-3和qPro-14-1，1个脂肪含量位点为qOil-16-1，贡献率为8.41%~17.83%。通过基因注释，在6个物理区间内分别筛选到26，29，6，1，124和36 个与脂肪和蛋白贮藏、油脂生物合成、油分代谢过程、脂肪酸生物合成、种子发育和种子萌发相关的基因。在定位的6个不同位点中，与脂肪含量相关的QTL分别位于7，16和19号染色体，与蛋白质含量相关的QTL分别位于 11 和 14 号染色体。有4个位点与前人定位结果相似,qPro-11-2 和 qPro-14-1为新位点。
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备注/Memo
收稿日期：2020-12-04