[1]王鹏,侯思宇,温宏伟,等.干旱胁迫对滞绿大豆种子萌发的影响及芽期抗旱性评价[J].大豆科学,2021,40(01):68-74.[doi:10.11861/j.issn.1000-9841.2021.01.0068]
　WANG Peng,HOU Si-yu,WEN Hong-wei,et al.Effects of Drought Stress on Seed Germination and Evaluation of Drought Resistance in Bud Stage of Stay-green Soybean[J].Soybean Science,2021,40(01):68-74.[doi:10.11861/j.issn.1000-9841.2021.01.0068]
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干旱胁迫对滞绿大豆种子萌发的影响及芽期抗旱性评价

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第40卷 期数: 2021年01期 页码: 68-74 栏目: 出版日期: 2021-01-20
Title:
Effects of Drought Stress on Seed Germination and Evaluation of Drought Resistance in Bud Stage of Stay-green Soybean
文章编号:
2021,40(1)：068-074
作者:
王鹏; 侯思宇; 温宏伟; 李贵全
（1.山西农业大学 农学院，山西 太谷 030801;2.山西农业大学 农业生物工程研究所,山西 太谷 030801）
Author(s):
WANG Peng;  HOU Si-yu;  WEN Hong-wei;  LI Gui-quan
(1.College of Agriculture, Shanxi Agricultural University, Taigu 030801, China; 2.Institute of Agricultural Biotechnology, Shanxi Agricultural University, Taigu 030801, China)
关键词:
滞绿大豆;  PEG胁迫;  萌发性状;  芽期抗旱性
Keywords:
Stay-green variety;  PEG osmotic stress;  Germination traits;  Drought resistance in bud stage
DOI:
10.11861/j.issn.1000-9841.2021.01.0068
文献标志码:
A
摘要:
为研究干旱胁迫对滞绿大豆种子萌发性状的影响，以滞绿和非滞绿常规大豆品种（系）为供试材料，通过PEG模拟干旱胁迫，对不同基因型大豆种子萌发性状进行了对比分析，并对其抗旱性进行了综合评价。结果表明：滞绿大豆品种在干旱胁迫中后期能维持较高的吸水率，失水时间较常规品种晋大74延迟6~12 h。PEG胁迫显著降低了大豆种子的发芽率、发芽势、培根长、下胚轴长、胚根干(鲜)重及贮藏物质转运率。滞绿突变体stg在胁迫过程中吸水率最大，但发芽能力较弱。stg诱变后代品系M9种子相对发芽势、相对发芽率和萌发抗旱系数均大于其它品种（系）。常规品种晋大74根系的伸长受PEG胁迫的影响最大，相对下胚轴长和相对胚根长显著小于晋大滞绿1号和stg诱变后代品系。晋大滞绿1号相对胚根长和胚根/下胚轴指数分别为0.85和2.38，显著大于其它品种（系），表明其在PEG胁迫下会优先促进胚根的伸长。通过隶属函数值对不同大豆品种（系）进行芽期抗旱性综合评价，结果表明晋大滞绿1号为强抗旱类型，其杂交母本晋大74为中等抗旱品种。
Abstract:
To study the effect of drought stress on the seed germination of stay-green soybean, the germination characteristics of different soybean seeds were compared and analyzed under PEG osmotic stress, and their drought resistance were evaluated comprehensively. The results showed that the stay-green soybean could maintain higher water absorption rate in the middle and late stage of PEG stress, and the time of loss water delayed 6-12 h compared with the common variety Jinda 74. PEG osmotic stress significantly reduced the germination rate, germination energy, radicle length, hypocotyl length, radicle dry (fresh) weight and the storage substance transportation rate of soybean seeds. Stay-green mutant stg had the highest water absorption rate but the weakest germination rate under PEG osmotic stress. The relative germination energy, relative germination rate and relative germination index ofM9were all higher than those of the other varieties (lines). The root elongation of the common variety Jinda 74 was the most affected by PEG osmotic stress, and the relative hypocotyl length and relative radicle length were significantly lower than those of the other varieties (lines). The relative radicle length and relative radicle length/relative hypocotyl length ratio of Jinda stay-green 1 were 0.82 and 2.38 respectively, significantly higher than those of the other varieties (lines), indicating that it priority to promoted radicle elongation under PEG stress. The evaluation on drought resistance using subordinate function values showed that stay-green was a type of strong drought resistance and Jinda stay-green 1 was a type of medium drought resistance. The results of this study are significant to the soybean germplasm innovation and the breeding of drought-resistant varieties.

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备注/Memo

国家重点研发计划(2017YFE0111000); 欧盟H2020研究创新项目(727312); 山西农业大学青年创新项目（20142-03）。

更新日期/Last Update: 2021-02-09