ZHOU Qi-zheng,ZHONG Xuan-bo,CUI Nan,et al.Evaluation of Herbicide Tolerance and Effects on Arthropods in the Field of Transgenic Soybean with Glyphosate Resistance G1 and G2[J].Soybean Science,2020,39(06):875-881.[doi:10.11861/j.issn.1000-9841.2020.06.0875]
抗草丁膦G1和G2转基因大豆对除草剂耐性评价及田间节肢动物影响研究
- Title:
- Evaluation of Herbicide Tolerance and Effects on Arthropods in the Field of Transgenic Soybean with Glyphosate Resistance G1 and G2
- 文献标志码:
- A
- 摘要:
- 摘要：为评估转基因大豆的环境生态风险，本研究在田间和室内评价了抗草丁膦转基因大豆G1和G2对靶标除草剂草丁膦和非靶标除草剂草甘膦、灭草松和烯禾啶的抗性，同时明确了G1和G2对田间节肢动物多样性的影响。结果表明：在田间喷施1、2和4倍浓度的靶标除草剂草丁膦后14 d，G1和G2均能正常生长，株高随喷施浓度增加略有下降，分别为33.8，31.9，29.1 cm和32.9，30.3，28.3 cm，但与G1、G2清水对照和转基因受体天隆一号清水对照相比无显著差异；喷施后28 d，G1和G2植株均能正常生长且株高有所增加；喷施不同浓度草丁膦表现为转基因受体受害率平均为99.4%~100%，而G1和G2平均受害率为0.8%~6.8%，且随着喷施时间的延长，G1和G2的受害率有所减缓。喷施不同浓度非靶标除草剂草甘膦和灭草松后，转基因大豆G1和G2及受体大豆均出现明显药害症状，受害率为92.3%~100%，且于喷施28 d后植株全部死亡；而喷施不同浓度非靶标除草剂烯禾啶，G1、G2及受体大豆均能正常生长，平均受害率为10%以下，植株株高与清水对照无显著差异。结果表明G1和G2大豆只对靶标除草剂产生耐性，而不受非靶标除草剂影响。同时，种植转基因G1、G2及受体大豆对田间节肢动物不存在生态风险。
- Abstract:
- In order to assess the environmental and ecological risk in transgenic glyphosate-resistance soybean G1 and G2, the resistance of target herbicides glufosinate and non-target herbicides glyphosate, bentazone and dimethoate were evaluated under field and indoor experiments, and the effects of G1 and G2 on arthropod biodiversity in the field were also observed. The results indicated that G1 and G2 could grow normally after 14 d of spraying 1, 2 and 4 times concentration target herbicide glufosinate and the plant height decreased a little with the increasing of the concentration. The plant height of G1 and G2 were 33.8, 31.9, 29.1 cm and 32.9, 30.3, 28.3 cm, respectively, 14 and 28 d after spraying 1, 2 and 4 times glufosinate, but there was no significant difference compared with control and receptor Tianlong 1. The damage rate of transgenic receptor was 99.4%-100% in average 14 and 28 d after spraying glufosinate, while that of G1 and G2 was only 0.8%-6.8%. Furthermore, the damage rate of G1 and G2 was alleviated with the prolong of spraying time. The G1, G2 and soybean receptor showed the same phytotoxicity after spraying different concentrations of non-target herbicides glyphosate and Bentazon. The damage rate ranged from 92.3% to 100% and all the plants died 28 d after spraying. However, G1, G2 and receptor could grow normally, the damage rate in average was less than 10% and there was no significant difference in plant height with control after spraying different concentrations of non-target herbicides sethoxydim. At the same time, the results showed there were no ecological impact on biodiversity of arthropods in the field when the G1 and G2 were growing.
参考文献/References:
［1］刘爱秋,邓晓建,吴成,等. 转基因作物的安全性问题及对策［J］. 中国农学通报,2002,18(5):70-73. (Liu A Q, Deng X J, Wu C, et al. Study on the problems and strategies of biosafety on genetically modified crops［J］. Chinese Agricultural Science Bulletin, 2002, 18(5):70-73.)［2］Arujanan.ISAAA in 2019 accomplishment report［EB/OL］. http://www.isaaa.org/resources/publications/annualreport/2019/pdf/ISAAA-2019-Accomplishment-Report.pdf. ［3］赵方方. 抗虫耐除草剂转基因玉米吉抗309的环境安全评价和营养成分分析［D］. 哈尔滨: 哈尔滨师范大学, 2018. (Zhao F F. Environmental safety evaluation and quality analysis of insect-resistant and herbicide transgenic maize Jikang 309 ［D］.Harbin: Harbin Normal University, 2018.)［4］张宏军,刘学,张佳,等.草铵膦的作用机理及其应用［J］.农药与科学管理, 2004, 25(4): 23-27. (Zhang H J, Liu X, Zhang J, et al. Mechanism and utilization of glufosinate-ammonium［J］. Pesticide Science and Administration, 2004, 25(4): 23-27.)［5］华乃震. 非选择性除草剂的进展和应用［J］. 农药市场信息, 2011(21):19-20. (Hua N Z. Progress andapplication of non-selective herbicides［J］. Pesticide Market News, 2011(21):19-20.)［6］李凡,孙红伟,杨淑珂,等.转植酸酶基因玉米对田间节肢动物群落多样性的影响［J］.生物安全学报,2014,23(4):265-270.(Li F, Sun H W, Yang S K, et al. Effects of phytase transgenic maize on biodiversity of arthropod communities under field conditions［J］. Journal of Biosafety, 2014, 23(4):265-270.)［7］陈伟,李娜,曹越平.抗草甘膦转基因大豆SHZD32-01田间节肢动物和杂草多样性研究［J］.上海交通大学学报（农业科学版）,2019, 37(1):54-60. (Chen W, Li N, Cao Y P. Assessment of glyphosate-resistant transgenic soybean SHZD32-01 on biodiversity of arthropod and weed［J］. Journal of Shanghai Jiaotong University(Agricultural Science), 2019, 37(1): 54-60.)［8］卢涛,李红艳,章洁琼,等. 以bar基因为筛选标记转基因大豆的获得及鉴定［J］. 大豆科学, 2011, 30(6):895-900. (Lu T, Li H Y, Zhang J Q, et al. Acquisition and identification of transgenic soybean using bar as a selective agent［J］. Soybean Science, 2011, 30(6): 895-900.)［9］李红艳. 抗胞囊线虫转基因大豆种质创新研究［D］. 杭州: 浙江大学, 2013. (Li H Y. Studies on the germplasm innovation of soybean (Glycine max L.) cyst-nematod resistance breeding［D］. Hangzhou: Zhejiang University, 2013.)［10］徐鵾鸬, 冯卫虎, 晁倩林,等. 不同种类粘虫板悬挂密度对茶小绿叶蝉种群数量的影响［J］. 茶叶学报, 2019, 60(3):119-121.(Xu K L, Feng W H, Chao Q L, et al. Pest trapping at tea plantation as affected by trap type and hanging density［J］. Acta Tea Sibica, 2019, 60(3):119-121.)［11］刘阳, 姜洪, 袁忠林, 等. 粘虫板对苜蓿田牛角花齿蓟马的诱集效果［J］.山东农业科学, 2019,51(7): 83-86.(Liu Y, Jiang H, Yuan Z L, et al. Attractive effects of sticky boards on odonotothrips loti in alfalfa field［J］. Shandong Agricultural Sciences, 2019,51(7): 83-86.)［12］曹洪玉,李香菊,刘士阳,等. 抗除草剂大豆356043对几种除草剂的耐受性评价［J］. 杂草学报, 2011, 29(2): 32-35.(Cao H Y, Li X J, Liu S Y, et al. Assessment of herbicide tolerances of transgenic herbicide-resistant soybean variety 356043［J］. Weed Science, 2011, 29(2): 32-35.)［13］姜伟丽,马小艳,任相亮,等.除草剂草铵膦对转基因抗草铵膦棉花生长发育的影响［J］.生物安全学报,2016,25(4): 286-290. (Jiang W L, Ma X Y, Ren X L, et al. Effects of glufosinate on the growth and development of transgenic glufosinate-resistant cotton［J］. Journal of Biosafety, 2016, 25(4):286-290.)［14］Lanelos D Y,Webster E P, Zhang W, et al. Response of glufosinate-resistance rice to glufosinate application timings［J］. Weed Technology, 2003(17): 157-160.［15］卜贵军,刘洪梅,李英,等.草甘膦对大豆超微结构及光合指标影响的研究［J］. 电子显微学报,2008, 27(4):322-330. (Bu G J, Liu H M, Li Y et al. Soybean ultrastructure and photosynthesis index as affected by glyphosate［J］. Journal of Chinese Electron Microscopy Society, 2008, 27(4): 322-330.)［16］张宏军.转基因水稻品系99-1对草铵膦抗性及竞争性的评价［D］. 北京:中国农业大学,2003. (Zhang H J. Evaluation of transgenic rice line 99-1 in tolerance to glufosinate and its competition against barnyardgrass［D］. Beijing: China Agricultural University, 2003.)［17］Marques L H, Santos A C, Castro B A, et al. Impact of transgenic soybean expressing Cry1Ac and Cry1F proteins on the non-target arthropod community associated with soybean in Brazil［J］. PLoS One, 2018, 13(2): e0191567.［18］张卓,刘二明,黄文坤,等. 抗草甘膦转基因大豆对豆田主要杂草多样性的影响［J］.湖南农业科学,2011(14):30-31. (Zhang Z, Liu E M, Huang W K, et al. Effects of glyphosate-resistant transgenic soybean on the diversity of main weeds in soybean fields［J］. Hunan Agricultural Sciences,2011(14):30-31.)［19］郭井菲,张聪,袁志华,等.转〖STBX〗cry1Ie〖STBZ〗基因抗虫玉米对田间节肢动物群落多样性的影响［J］. 植物保护学报, 2014,41(4): 482-489. (Guo J F, Zhang C, Yuan Z H, et al. Effect of 〖STBX〗cry1Ie〖STBZ〗 transgenic maize on the diversity of arthropod community in the field［J］. Journal of Plant Protection, 2014, 41(4): 482-489.)［20］康岭生,马瑞,杨向东,等.高油酸转基因大豆HOA80对生物多样性影响的检测［J］.吉林农业科学,2014,39(2):33-36. (Kang L S, Ma R, Yang X D, et al. Assess of impact of high oleic transgenic soybean HOA80 on biodiversity［J］. Journal of Jilin Agricultural Sciences, 2014, 39(2): 33-36.)
备注/Memo
收稿日期：2020-04-22