JIANG Hai-peng,TIAN Li-zheng,BU Fan-shan,et al.Bioinformatics Analysis of bZIP Transcription Factors Related to Resistance to Soybean Cyst Nematode[J].Soybean Science,2020,39(05):703-711.[doi:10.11861/j.issn.1000-9841.2020.05.0703]
大豆胞囊线虫病抗性相关bZIP转录因子的生物信息学分析
- Title:
- Bioinformatics Analysis of bZIP Transcription Factors Related to Resistance to Soybean Cyst Nematode
- Keywords:
- Soybean cyst nematode; Race 3; Transcriptome sequencing; bZIP transcription factors; Bioinformatics
- 文献标志码:
- A
- 摘要:
- 为分析大豆bZIP转录因子在大豆胞囊线虫病3号生理小种胁迫下的应答机制，促进大豆响应大豆胞囊线虫病3号生理小种胁迫机制的研究，本研究采用胞囊线虫病3号生理小种对抗线虫大豆品种东农L-10进行胁迫，对转录组测序数据中与抗性相关的bZIP转录因子进行在线生物信息学预测和分析。结果共获得18个与大豆胞囊线虫病3号生理小种相关的差异表达bZIP转录因子，不同bZIP转录因子之间的理化性质存在较大差异，大多数上调表达的bZIP转录因子为碱性等电点，而大多数下调表达的bZIP转录因子为酸性等电点；18个bZIP蛋白中含有多个丝氨酸(S)、苏氨酸(T)和酪氨酸(Y)磷酸化位点，其中丝氨酸(S)位点最多；亚细胞定位显示2个bZIP转录因子定位于叶绿体，其余均定位于细胞核，与转录因子调控下游基因的表达特性相一致；18个bZIP蛋白的系统进化树分为2大类群，其中上调表达的bZIP转录因子和下调表达的bZIP转录因子亲缘关系较远；bZIP基因家族的成员都含有数量不等的motif结构，且不同bZIP基因之间保守结构域的氨基酸存在变异；蛋白二级结构中以无规则卷曲和α-螺旋为主要构成元件；根据结构相似性，18个bZIP蛋白三级结构分为4类，不同bZIP转录因子三级结构的相似性暗示了功能上存在相似。
- Abstract:
- ?In order to analyze the response mechanism of soybean bZIP transcription factor to the stress of soybean cyst nematode race 3, and to lay a foundation for studying the mechanism of soybean responding to the stress of soybean cyst nematode race 3. In this study, the resistant soybean variety Dongnong L-10 was stressed by cyst nematode race 3, and the bZIP transcription factors related to resistance in transcriptome sequencing data were predicted and analyzed by online bioinformatics. The results showed that 18 differentially expressed bZIP transcription factors related to soybean cyst nematode race 3 were obtained, and there were differences in physical and chemical properties among different transcription factors. Most of the up-regulated bZIP transcription factors were basic isoelectric points, while most of the down-regulated bZIP transcription factors were acidic isoelectric points. Among the 18 bZIP proteins, there were multiple serine (S), threonine (T) and tyrosine (Y) phosphorylation sites, the most of which were serine (S). Subcellular localization showed that two bZIP transcription factors were located in chloroplast and the rest were located in nucleus, which was consistent with the expression characteristics of downstream genes regulated by transcription factors. According to the phylogenetic tree of bZIP transcription factor proteins, 18 bZIP transcription factors were divided into two groups, in which the up-regulated bZIP transcription factors and down-regulated bZIP transcription factors were closely related. Members of the bZIP gene family all contained a number of motif structures, and there were variations in the amino acids of the conserved domains between different bZIP genes. The protein secondary structure was mainly composed of random coil and α-helix. According to the structural similarity, the tertiary structures of 18 bZIP proteins were divided into 4 categories. The similarity of the tertiary structure of different bZIP transcription factors suggests that there are functional similarities. This study lays a theoretical foundation for elucidating the mechanism of soybean bZIP transcription factor in response to stress of soybean cyst nematode race 3, and provides a theoretical basis for improving the resistance of soybean cyst nematode.
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备注/Memo