ZHOU Yuan-yuan,GUO Yong-xia,DUAN Yu-xi,et al.Prevention Effect and Photosynthetic Performance in Bacillus Megaterium Sneb207 Against Soybean Cyst Nematode[J].Soybean Science,2020,39(04):605-611.[doi:10.11861/j.issn.1000-9841.2020.04.0605]
巨大芽孢杆菌Sneb207诱导大豆抗胞囊线虫病的防效及光合响应
- Title:
- Prevention Effect and Photosynthetic Performance in Bacillus Megaterium Sneb207 Against Soybean Cyst Nematode
- 关键词:
- 巨大芽孢杆菌Sneb207; 大豆胞囊线虫; 光合性能; 差异表达基因
- Keywords:
- Bacillus megaterium Sneb207; Soybean cyst nematode; Photosynthetic performance; Differentially expressed genes
- 文献标志码:
- A
- 摘要:
- 为明确巨大芽孢杆菌Sneb207对大豆抗胞囊线虫的防效及对大豆早期光合作用的影响，采用盆栽试验，通过Sneb207发酵液包衣感病大豆品种，以无菌水包衣为对照，接种大豆胞囊线虫，于显囊期调查植株生长指标及Sneb207对胞囊的防效，并且分别于接种线虫后3,7,10和14 d测定Sneb207包衣处理和对照处理大豆的光合性能及叶绿素含量。结合前期转录组分析结果，选择4个与光合作用或叶绿体相关基因进行qRT-PCR验证。结果表明：Sneb207包衣处理，大豆主根长增加且须根数量增多，同时提高了对胞囊的防效，根上和土中胞囊抑制率分别达到44.93%和57.19%。Sneb207包衣处理提高了大豆的净光合速率和叶绿素含量。所检测的4个基因在Sneb207诱导大豆抗胞囊线虫过程中2个被诱导、1个被抑制，且与对照处理差异显著，表明它们可能在Sneb207诱导大豆抗胞囊线虫中起作用。
- Abstract:
- To determine the effectiveness of Bacillus megaterium Sneb207 against soybean cyst nematodes and early photosynthesis in soybean, the seed coating technology was used to coat the susceptible soybean varieties, and the sterile water coating was used as a control treatment in greenhouse experiments. We surveyed the cysts on the soybean roots and in the rhizosphere soil, the influence on soybean growth after inoculation 30-35 d, and measured the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), intercellular CO2 concentration (Ci) and chlorophyll content after inoculated 3, 7, 10 and 14 d. We found that, in the Sneb207 coated and nematode inoculated treatment, the main root length and the number of fibrous roots of soybean were increased, and the soybean cyst nematode outside the soybean roots and in rhizosphere soil were suppressed, the inhibition rates of cysts reached 44.93% and 57.19%, respectively. Further, the Sneb207 coated treatment increased the Pn and chlorophyll content. In addition, in the four genes, there were two genes up-regulated and one gene down-regulated expression when the resistance to SCN of soybean was induced by Sneb207 , and there were significant difference with control, indicating that they may play a role in Sneb207-induced resistance to SCN of soybean.
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