LU Jie-chun,MOU Bao-min,ZHENG Dian-feng,et al.Effects of 5-aminolevulinic Acid on Biomass and Physiological Characteristics of Soybean Seedlings Under Saline-alkali Stress[J].Soybean Science,2020,39(01):84-89.[doi:10.11861/j.issn.1000-9841.2020.01.0084]
5-氨基乙酰丙酸对盐碱胁迫下大豆苗期生物量及生理特性的影响
- Title:
- Effects of 5-aminolevulinic Acid on Biomass and Physiological Characteristics of Soybean Seedlings Under Saline-alkali Stress
- 文献标志码:
- A
- 摘要:
- 为研究5-氨基乙酰丙酸对大豆苗期盐碱胁迫的缓解效应，本研究以大豆品种合丰50为材料，采用盆栽方法，研究了40 mg·L-1的5-氨基乙酰丙酸拌种对盐碱胁迫下苗期大豆生长发育的影响。结果表明：与正常情况相比，盐碱胁迫抑制大豆生长：大豆生物积累量、叶片相对含水量和叶绿素含量分别降低了32.69%、9.9%和14.0%。与盐碱胁迫条件相比，5-氨基乙酰丙酸处理能有效缓解生物量、叶片相对含水量和叶绿素含量的降低，超氧化物歧化酶、过氧化物酶、过氧化氢酶和脯氨酸含量分别提高了13.3%、23.2%、23.0%和56.0%，相对电导率降低了3.08%。研究表明，5-氨基乙酰丙酸能缓解盐碱胁迫对大豆幼苗的损伤，提高大豆耐盐碱性。
- Abstract:
- In order to study the effect of 5-aminolevulinic acid on saline-alkali stress in soybean seedlings, a pot experiment was conducted to study the effects of 5-aminolevulinic acid seed dressing on the growth and development of soybean seedlings under saline-alkali stress with Hefeng 50 as the experimental material and 40 mg·L-1 as the dosage. The results showed that compared with normal conditions, saline-alkali stress inhibited soybean growth, soybean bioaccumulation, leaf relative water content and chlorophyll content decreased by 32.69%, 9.9% and 14.0%, respectively. Compared with saline-alkali stress conditions, 5-aminolevulinic acid treatment can effectively alleviate the decrease of biomass, leaf relative water content and chlorophyll content, superoxide dismutase, peroxidase, catalase and proline. The content increased by 13.3%, 23.2%, 23.0% and 56.0%,respectively, and the relative conductivity decreased by 3.08%.In summary,5-aminolevulinic acid can alleviate the damage of soybean seedlings caused by saline-alkali stress and improve the saline-alkali tolerance of soybean.
参考文献/References:
［1］Jampeetong A,Brix H.Effects of NaCl salinity on growth, morphology, photosynthesis and proline accumulation of Salvinia natans［J］. Aquatic Botany, 2009, 91(3):181-186.［2］ 张磊,侯云鹏,王立春.盐碱胁迫对植物的影响及提高植物耐盐碱性的方法［J］.东北农业科学,2018,43(4):11-16.(Zhang L,Hou Y P,Wang L C.Effect of saline-alkali stress on plants and methods for improving salt and alkali resistance of plants［J］.Northeast Agricultural Sciences,2018,43(4):11-16.)［3］ Munns R.Comparative physiology of salt and water stress［J］.Plant Cell and Environment,2002,25(2):239-250.［4］ Yang J Y, Zheng W, Tian Y, et al. Effects of various mixed salt-alkaline stresses on growth, photosynthesis, and photosynthetic pigment concentrations of Medicago ruthenica, seedlings［J］. Photosynthetica, 2011, 49(2):275-284.［5］ Zhang J T, Mu C S. Effects of saline and alkaline stresses on the germination, growth, photosynthesis, ionic balance and antioxidant system in an alkali-tolerant leguminous forage Lathyrus quinquenervius［J］. Soil Science & Plant Nutrition, 2010, 55(5):685-697.［6］ Yang C, Chong J, Li C, et al. Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana, during adaptation to salt and alkali conditions［J］. Plant & Soil, 2007, 294(1-2):263-276.［7］ 吴杨,高慧纯,张必弦,等.24-表油菜素内酯对盐碱胁迫下大豆生育、生理及细胞超微结构的影响［J］.中国农业科学,2017,50(5): 811-821.(Wu Y, Gao H C, Zhang B X, et al. Effect of 24-epibrassinolide on growth, physiology and ultrastructure of soybean under salt-alkalistress［J］.Scientia Agricultura Sinica,2017,50(5):811-821.)［8］ 邵帅. 盐碱胁迫下野生大豆幼苗光合特性与离子动态平衡研究［D］. 长春：东北师范大学,2012.（Shao S. Study on photosynthetic characteristics and ion dynamic equilibrium of wild soybean seedlings under salt-alkali stress ［D］. Changchun: Northeast Normal University, 2012.）［9］ 季平, 张鹏, 徐克章,等.不同类型盐碱胁迫对大豆植株生长性状和产量的影响［J］.大豆科学,2013,32(4):477-481.（Ji P, Zhang P, Xu K Z, et al. Effects of different salt and alkali stresses on growth traits and yield of soybean plants ［J］.Soybean Science, 2013, 32 (4): 477-481.）［10］Nishihara E, Kondo K, Parvez M M, et al. Role of 5-aminolevulinic acid (ALA) on active oxygen-scavenging system in NaCl-treated spinach (Spinacia oleracea)［J］. Journal of Plant Physiology, 2003, 160(9):1085-1091.［11］汪良驹,姜卫兵,章镇,等.5-氨基乙酰丙酸的生物合成和生理活性及其在农业中的潜在应用［J］.植物生理学通讯,2003(3):185-192.(Wang L J, Jiang W B, Zhang Z, et al. Biosynthesis and physiological activity of 5-aminolevulinic acid and its potential application in agriculture［J］.Plant Physiology Communications,2003(3):185-192.)［12］Hotta Y, Tanaka T, Takaoka H, et al. Promotive effects of 5-aminolevulinic acid on the yield of several crops ［J］. Plant Growth Regulation, 1997, 22(2):109-114.［13］Zhang C P, Li Y C, Yuan F G, et al. Role of 5-aminolevulinic acid in the salinity stress response of the seeds and seedlings of the medicinal plant Cassia obtusifolia L.［J］. Botanical Studies, 2013, 54(1):18.［14］Balestrasse K B, María L Tomaro, Batlle A, et al. The role of 5-aminolevulinic acid in the response to cold stress in soybean plants［J］. Phytochemistry, 2010, 71(17-18):2038-2045.［15］Aksakal O, Algur O, Aksakal F I, et al. Exogenous 5-aminolevulinic acid alleviates the detrimental effects of UV-B stress on lettuce (Lactuca sativa L) seedlings［J］. Acta Physiologiae Plantarum, 2017, 39(2):55.［16］邹琦.植物生理学实验指导［M］.北京:中国农业出版社,2000,56.（Zou Q. Experimental guide to plant physiology M. Beijing: China Agricultural Press, 2000,56.）［17］赵世杰,史国安,董新纯.植物生理学实验指导［M］.北京:中国农业科学技术出版社,2002：34.（Zhao S J, Shi G A, Dong X C. Experimental guidance of plant physiology ［M］. Beijing: China Agricultural Science and Technology Press, 2002：34.）［18］Blum A, Ebercon A. Cell membrane stability as a measure of drought and heat tolerance in wheat［J］. Crop Science, 1981, 21(1):43-47.［19］中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南［M］.北京:科学出版社,1999：127.（Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai Society of Plant Physiology. Experimental guide to modern plant physiology ［M］. Beijing: Science Press, 1999：127.）［20］王晶英,敖红,张杰,等.植物生理生化实验技术与原理［M］.哈尔滨:东北林业大学出版社,2003:7.(Wang J Y, Ao H, Zhang J, et al. Experimental techniques and principles of plant physiology and biochemistry ［M］. Harbin: Northeast Forestry University Press, 2003: 7.)［21］李合生.植物生理生化实验原理和技术［M］.北京：高等教育出版社,2000:267-268.(Li H S. Principles and techniques of plant physiology and biochemistry experiment ［M］. Beijing:Higher Education Press, 2000: 267-268.)［22］Lissner J, Schierup H H, ComíN F A, et al. Effect of climate on the salt tolerance of two Phragmites australis, populations:I.Growth, inorganic solutes,nitrogen relations and osmoregulation［J］. Aquatic Botany, 1999, 64(3-4):335-350.［23］张春平,何平, 韦品祥,等.外源5-氨基乙酰丙酸对盐胁迫下紫苏种子萌发及幼苗抗氧化酶活性的影响［J］.中草药,2011,42(6):1194-1200.(Zhang C P,He P,Wei P X,et al. Effects of exogenous 5-aminolevulinic acid on seed germination and antioxidant enzyme activities of Perilla frutescens under salt stress［J］.Chinese Traditional and Herbal Drugs,2011,42(6):1194-1200.)［24］潘瑞炽,王小菁,李娘辉,等.植物生理学［M］.北京:高等教育出版社,2012：323.(Pan R Z, Wang X J, Li N H, et al. Plant Physiology ［M］. Beijing: Higher Education Press, 2012：323.)［25］李芳芳. 外源5-氨基乙酰丙酸对酸枣种子及幼苗NaCl胁迫缓解效应的研究［D］. 新疆：石河子大学,2017.（Li F F. The alleviation effect of exogenous 5-aminolevulinic acid on NaCl stress of wild jujube seeds and seedlings ［D］. Xinjiang: Shihezi University, 2017.）［26］李利博,张浩,叶嘉,等.盐胁迫对白鹤芋生理指标和叶绿素含量的影响［J］.北方园艺,2018(15):103-108.(Li L B,Zhang H,Ye J,et al.Effects of salt stress on physiological indexes and chlorophyll content of white branches［J］.Northern Horticulture, 2018(15):103-108.)［27］宋士清,郭世荣.5-氨基乙酰丙酸的生理作用及其在农业生产中的应用(综述)［J］.河北科技师范学院学报,2004(2):54-57,72.(Song S Q,Guo S R.The physiological function of 5-aminolevulinic acid and its application in agricultural production （Review）［J］.Journal of Hebei Normal University of Science and Technology,2004(2):54-57,72.)［28］Wu Y, Jin X, Liao W, et al. 5-Aminolevulinic acid (ALA) alleviated salinity stress in cucumber seedlings by enhancing chlorophyll synthesis pathway［J］. Frontiers in Plant Science, 2018, 9:635.［29］Asada K. Thewater-watercycle inchloroplasts: Scavenging of active oxygens and dissipation of excess photons［J］. Annual Reviews Plant Physiology Plant Molecular Biology, 1999, 50(50):601-639.［30］Liau Y J, Wen L, Shaw J F, et al. A highly stable cambialistic-superoxide dismutase from Antrodia camphorata: Expression in yeast and enzyme properties［J］. Journal of Biotechnology, 2007, 131(1):84-91.［31］Gadallah M A A. Effects of proline and glycinebetaine on vicia faba responses to salt stress［J］. Biologia Plantarum, 1999, 42(2):249-257.
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备注/Memo
收稿日期：2018-12-07