AN Qi,WU Hao-qiong,YIN Bo,et al.Effect of nodVW Genes on Symbiotic Nitrogen Fixation of Sinorhizobium fredii HH103[J].Soybean Science,2018,37(06):871-875.[doi:1011861/jissn1000-98412018060871]
导入nodVW基因对费氏中华根瘤菌HH103共生固氮的影响
- Title:
- Effect of nodVW Genes on Symbiotic Nitrogen Fixation of Sinorhizobium fredii HH103
- Keywords:
- Two component regulatory system; nodVW; Recombinant rhizobium; Nodulation and nitrogen fixation
- 文献标志码:
- A
- 摘要:
- 摘要：本研究以慢生型根瘤菌(Bradyrhizobium japonicum) USDA110为出发菌株，扩增慢生型根瘤菌中双组份调控基因nodVW，并将其导入快生型根瘤菌(Sinorhizobium fredii) HH103及HH103 nodD1失活突变株中，研究重组根瘤菌菌株对大豆嫩丰16共生固氮的影响。结果表明：慢生型大豆根瘤菌双组份调控系统NodVW的导入使大豆植株的株高和鲜重显著降低，大豆植株根部结瘤数量明显减少，NodVW的导入阻碍了快生型根瘤菌的结瘤固氮途径，从而抑制了快生型根瘤菌与大豆结瘤。
- Abstract:
- In this study, Bradyrhizobium japonicum USDA110 was used as the starting strain The two component regulatory genes nodVW from Bradyrhizobium japonicum USDA110 were amplified and introduced to Sinorhizobium fredii HH103 and HH103 nodD1 mutant strains in order to study the effect of nodVW on symbiotic nitrogen fixation of the recombinant strains to soybean genotype NF-16 The results showed that the introduction of the two-component control system NodVW from Bradyrhizobium japonicum USDA110 exert negative effect on soybean plants, which significantly reduced the height and fresh weight of soybean plants In the meanwhile, the number of nodules at soybean roots decreased significantly The results indicated that the introduction of NodVW hindered the pathway and inhibits the nodulation and nitrogen fixation of Sinorhizobium fredii HH103 accordingly
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备注/Memo
收稿日期：2018-08-03