ZHANG Cui,BAI Hui-yu,QU Chun-bo,et al.Study on Composite System of Positively Charged Soy Protein Isolate and Acrylic Acid[J].Soybean Science,2018,37(01):136-140.[doi:10.11861/j.issn.1000-9841.2018.01.0136]
荷正电大豆蛋白和丙烯酸的复合体系研究
- Title:
- Study on Composite System of Positively Charged Soy Protein Isolate and Acrylic Acid
- 关键词:
- 荷正电大豆蛋白（PCSPI）; 丙烯酸（AA）; 酶解
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 通过酶解大豆蛋白获得荷正电大豆蛋白，采用HPLC、Zeta电位、TEM对其酶解行为进行了追踪，进一步将酶解得到的大豆蛋白与丙烯酸复合，对复合物的溶液行为和微结构进行考察研究。结果显示: 酶解大豆蛋白后得到荷正电的球形粒子，其能够与丙烯酸通过静电作用形成球形的复合物SPI-AA纳米粒子，该球形粒子在加入引发剂引发聚合反应后粒径变小，在加入交联剂交联后粒径也随之变小。该纳米颗粒在药物负载、生物传感、水处理等领域有较好的应用前景。
- Abstract:
- Positively charged soy protein isolate（PCSPI）was prepared via the enzymatic hydrolysis of soy protein and the enzymatic hydrolysis process was traced by HPLC, Zeta and TEM. Then the composite of the positively charged soy protein isolate and acrylic acid（AA）was prepared, further the solution behavior and microstructure of the composite were studied. The result showed that the positively charged spherical particles were obtained after the enzymatic hydrolysis of soy protein. These particles could form spherical complex particles with AA through electrostatic interaction. The size of the complex particles became smaller with the addition of initiator or by crosslinking. Such nano-particles could be potentially used in the fields of drug loading, biosensor and water treatment.
参考文献/References:
［1］ Graiver D, Waikul L H, Berger C, et al. Biodegradable Soy protein-polyester blends by reactive extrusion process［J］. Journal of Applied Polymer Science, 2010, 92(5): 3231-3239.［2］ Liu D G, Tian H F, Jia X W. Effects of calcium carbonate polymorph on the structure and properties of soy protein-based nanocomposites［J］. Macromolecular Bioscience, 2010, 8(5): 401-409.［3］ Macdonald R S, Pryzbyszewski J, Hsieh F H. Soy protein isolate extruded with high moisture retains high nutritional quality［J］. Journal of Agricultural & Food Chemistry, 2009, 57(9): 3550-3555.［4］ Canabadyrochelle L S, Sanchez C, Mellema M, et al. Study of calcium-soy protein interactions by isothermal titration calorimetry and pH cycle［J］. Journal of Agricultural & Food Chemistry, 2009, 57(13): 5939-5947.［5］ Wang G H, Zhou A N, Hu X, et al. Effect of coal filler on the properties of soy protein plastics［J］. Journal of Applied Polymer Science, 2010, 102(4): 3134-3143. ［6］ Jiang J, Chen J, Xiong X L, et al. Structural and emulsifying properties of soy protein isolate subjected to acid and alkaline pH-shifting processes［J］. Journal of Agricultural & Food Chemistry, 2009, 57(16): 7576-7583.［7］ Wang E Y, Sanjeewa G, et al. Identification and characterization of topoisomerase II inhibitory peptides from soy protein hydrolysates［J］. Journal of Agricultural & Food Chemistry, 2008, 56, 6267-6277.［8］ Xu K K, Yao P. Stable oil-in-water emulsions prepared from soy protein-dextran conjugates［J］. Langmuir, 2009, 25(17): 9714-9720［9］ Huiyu B, Rakesh K, Yang C, et al. Effect of salicylic acid on the mechanical properties and water resistance of soy protein isolate films［J］. Smithers Rapra Technology, 2010,18(4):197-203［10］张翠, 徐晶, 白绘宇, 等. SPI / HA / CMC三元复合膜的制备及其性能研究［J］. 大豆科学, 2015, 34(4): 690-694. (Cui Z, Jing X, Bai H Y, et al. Preparation and study of SPI/HA/CMC ternary composite film［J］. Soybean Science, 2015, 34(4): 690-694.［11］Lam V D, Walker L M. A pH-induced transition of surfactant-polyelectrolyte aggregates from cylindrical to string-of-pearls structure［J］. Langmuir, 2010, 26(13): 10489-10496.［12］Hu Z, Jonas A M, Varshney S K, et al. Dilution-induced spheres-to-vesicles morphological transition in micelles from block copolymer/surfactant complexes［J］. Journal of the American Chemical Society, 2005, 127(18): 6526-6527.［13］Wang X Y, Li Y J, Li J X, et al. Salt effect on the complex formation between polyelectrolyte and oppositely charged surfactant in aqueous solution［J］. Journal of Physical Chemistry B, 2005, 109(21): 10807-10812.［14］Annaka M, Morishita K, Okabe S. Electrostatic self-assembly of neutral and polyelectrolyte block copolymers and oppositely charged surfactant［J］. Journal of Physical Chemistry B, 2007, 111(40): 11700-11707.［15］Yang J, Chen S, Fang Y. Viscosity study of interactions between sodium alginate and CTAB in dilute solutions at different pH values［J］. Carbohydrate Polymers, 2009, 75(2): 333-337.［16］Karlberg M, Piculell L, Huang L. Solubility of amylose/ionic surfactant complexes in dilute aqueous solutions: Dependence on surfactant concentration［J］. Carbohydrate Polymers, 2007, 70(3): 350-354.［17］白绘宇, 徐晶, 屈海军, 等. 大豆蛋白光敏接枝物SPI-g-P(VM-co-AMPS)的合成及溶液行为研究［J］. 大豆科学, 2011, 30(3): 475-479. (Bai H Y, Xu J, et al. Synthesis and solution behavior of photo-sensitive graft copolymers based on soy protein isolate［J］. Soybean Science, 2011, 30(3): 475-479.
备注/Memo
收稿日期：2017-08-13