[1]阚贵珍,张威,李亚凯,等.野生大豆芽期耐盐性状的关联分析[J].大豆科学,2017,36(05):733-736.[doi:10.11861/j.issn.1000-9841.2017.05.0737]
　KAN Gui-zhen,ZHANG Wei,LI Ya-kai,et al.Association Mapping of Wild Soybean (Glycine soja) Seed Germination Under Salt Stress[J].Soybean Science,2017,36(05):733-736.[doi:10.11861/j.issn.1000-9841.2017.05.0737]
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野生大豆芽期耐盐性状的关联分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年05期 页码: 733-736 栏目: 出版日期: 2017-09-20
Title:
Association Mapping of Wild Soybean (Glycine soja) Seed Germination Under Salt Stress
作者:
阚贵珍; 张威; 李亚凯; 喻德跃
（南京农业大学 大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏 南京 210095）
Author(s):
KAN Gui-zhen;  ZHANG Wei;  LI Ya-kai;  YU De-yue
(Soybean Research Institute, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China)
关键词:
野生大豆; 芽期; 耐盐; 关联分析
Keywords:
Wild soybean;  Germination;  Salt tolerance;  Genome-wide association mapping
DOI:
10.11861/j.issn.1000-9841.2017.05.0737
文献标志码:
A
摘要:
盐渍化是危害大豆生产的主要非生物胁迫因素之一。目前大豆耐盐性研究主要集中在栽培大豆的苗期耐盐性，而芽期耐盐性状的研究相对较少。野生大豆蕴含丰富的耐逆基因，是栽培大豆遗传改良的重要资源。为了研究野生大豆芽期耐盐性状的遗传机制，以113份野生大豆为试验材料，进行芽期耐盐性状的鉴定，结合群体的分子标记对包括2年平均值在内的3个环境下的3个耐盐指数进行全基因组关联分析，共检测到与野生大豆芽期耐盐相关的位点26个，6个SSR标记Satt521、Satt022、Satt239、Satt516 、Satt251和Satt285在2个或3个环境下均被检测到，4个SSR标记Satt516 、Satt251、Satt285和GMES4990与2个或3个耐盐指数显著相关。对这些SSR标记进行分析，挖掘了最优的等位基因及其载体材料。以上这些结果对于阐明野生大豆芽期耐盐性状的遗传机制，进一步发掘新的耐盐基因具有重要意义。同时也为栽培大豆遗传基础的拓宽、大豆耐盐分子标记辅助选择和分子设计育种等后续研究提供重要依据。
Abstract:
Salinity is one of major abiotic stress factors threatening soybean production. The previous studies mainly focused on salt tolerance at the soybean seedling stage. A few studies regarding salt tolerance during germination have been reported. Wild soybeans with richness of resistance genes are valuable resources for soybean genetic improvement. The objective of this study was to identify the genetic mechanisms of wild soybean seed germination under salt stress. One natural population consisting of 113 wild soybean accessions was used in this study. Three salt tolerance indices were measured. The molecular markers associated with salt tolerance were detected in 2013, 2014 and across two years by combining SSRs of genome-wide association analysis-26 SSR-trait associations were identified using a mixed linear model and the TASSEL 2-1 software. Six SSRs, Satt521, Satt022, Satt239, Satt516, Satt251 and Satt285, were identified in two or three environments. Four SSRs, Satt516, Satt251, Satt285 and GMES4990, were co-associated with two or three salt tolerance indices. Furthermore, elite alleles and their carrier materials were identified by analyzing alleles at the above loci. These results could contribute to elucidate the genetic mechanisms of salt tolerance, explore new salt tolerant genes, and provide important foundation for broadening soybean’s genetic base, molecular marker assisted selection and molecular design breeding in soybean salt tolerance breeding.

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备注/Memo

基金项目：中央高校基本科研业务费专项资金（KYZ201705）。

第一作者简介：阚贵珍（1978-），女，博士，副教授，主要从事大豆分子遗传育种研究。E-mail：kanguizhen@njau.edu.cn。
通讯作者：喻德跃（1965-），男，教授，博导，主要从事植物分子遗传与生物技术研究。E-mail：dyyu@njau.edu.cn。

更新日期/Last Update: 2017-10-31