[1]袁咏天,叶文武,曾丹丹,等.基于环介导等温扩增技术检测东北地区大豆主要品种（系）种子携带的病原菌[J].大豆科学,2017,36(04):592-597.[doi:10.11861/j.issn.1000-9841.2017.04.0592]
　YUAN Yong-tian,YE Wen-wu,ZENG Dan-dan,et al.Detection of Soybean Seed-borne Pathogens in Northeast China Using Loop-mediated Isothermal Amplification Assays[J].Soybean Science,2017,36(04):592-597.[doi:10.11861/j.issn.1000-9841.2017.04.0592]
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基于环介导等温扩增技术检测东北地区大豆主要品种（系）种子携带的病原菌

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年04期 页码: 592-597 栏目: 出版日期: 2017-07-20
Title:
Detection of Soybean Seed-borne Pathogens in Northeast China Using Loop-mediated Isothermal Amplification Assays
作者:
袁咏天; 叶文武; 曾丹丹; 王晓莉; 王源超; 郑小波
（南京农业大学 植物保护学院，江苏 南京 210095）
Author(s):
YUAN Yong-tian;  YE Wen-wu;  ZENG Dan-dan;  WANG Xiao-li;  WANG Yuan-chao;  ZHENG Xiao-bo
（Plant Protection College of Nanjing Agricultural University, Nanjing 210095, China）
关键词:
大豆病害; 种子带菌; 环介导等温扩增技术; 病原快速检测
Keywords:
Soybean disease;  Seed-borne pathogens;  LAMP(loop-mediated isothermal amplification);  Rapid detection of pathogen
DOI:
10.11861/j.issn.1000-9841.2017.04.0592
文献标志码:
A
摘要:
为了解东北地区大豆种子携带病原菌的情况，选取了该地区52个大豆主要品种（系），采用环介导等温扩增技术（loop-mediated isothermal amplification，LAMP）快速检测其种子携带胶孢炭疽菌、平头炭疽菌、冬青丽赤壳菌、亚细亚镰孢菌、黄色镰孢菌、木贼镰孢菌、禾谷镰孢菌、层出镰孢菌、尖镰孢菌、茄腐镰孢菌、轮枝镰孢菌、接骨木镰孢菌、大豆炭腐病菌、大豆拟茎点种腐病菌、大豆疫霉菌和立枯丝核菌16种大豆主要病原菌的状况。结果表明：在其中38个品种（系）的种子样本中累计检测出上述8种病原菌，检出率由高到低依次为：大豆拟茎点种腐病菌、立枯丝核菌、木贼镰孢菌、亚细亚镰孢菌、禾谷镰孢菌、层出镰孢菌、尖镰孢菌和平头炭疽菌。不同大豆品种（系）种子带菌的种类及数量存在较大差异，其中品种绥12-18和29182被检出的病原菌多达4种。本研究对了解东北地区大豆种子携带病原菌的状况有参考价值，并为大豆种子带菌检测提供了新的方法。
Abstract:
In order to know the situation of soybean seedborne pathogens in Northeast China, the seeds of 52 major soybean cultivars（lines） collected from this area were analyzed, using loop-mediated isothermal amplification(LAMP) assays for specific detection of 16 known soybean pathogen species, including .Colletotrichum gloeosporioide, C.truncatum, Calonectria ilicicola, Fusarium asiaticum, F.culmorum, F.equiseti, F. graminearum, F. oxysporum, F. proliferatum, F. solani, F. sambucinum, F. verticilliodes, Macrophomina phaseolina, Phomapsis longicolla, Phytophthora sojae, and Rhizoctonia solani. The result showed that there were 38 soybean cultivars(lines) carrying a total of 8 species of seed-borne pathogens, and the most frequent species was Phomapsis longicolla, followed by Rhizoctonia solani, Fusarium equiseti, F. asiaticum, F.graminearum, F. proliferatum, F. oxysporum and Colletotrichum truncatum.The number of detected species in each cultivar of seeds were between 1 and 4.In summary, a simple, rapid, and sensitive method for detecting soybean seed-borne pathogens was developed and successfully used to reveal the situation of soybean seed-borne pathogens in Northeast China.

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相似文献/References:

[1]马淑梅.黑龙江省大豆三大主要病害抗性鉴定方法研究[J].大豆科学,2007,26(05):744.[doi:10.3969/j.issn.1000-9841.2007.05.022]
　MA Shu-mei.METHODS ON RESISTANCE IDENTIFICATION OF THREE MAIN SOYBEAN DISEASES IN HEILONGJIANG PROVINCE[J].Soybean Science,2007,26(04):744.[doi:10.3969/j.issn.1000-9841.2007.05.022]
[2]魏思楠,朱晓峰,陈立杰,等.我国不同地区大豆主栽品种种子携带真菌研究[J].大豆科学,2014,33(06):890.[doi:10.11861/j.issn.1000-9841.2014.06.0890]
　WEI Si-nan,ZHU Xiao-feng,CHEN Li-jie,et al.Seed-Borne Fungi Detection in Different Soybean Cultivars[J].Soybean Science,2014,33(04):890.[doi:10.11861/j.issn.1000-9841.2014.06.0890]

备注/Memo

基金项目：国家公益性行业农业科研专项（201303018）；国家高技术研究发展计划“863计划”（2012AA101501）；国家现代农业产业技术体系（CARS-004-PS14）。

第一作者简介：袁咏天（1993-），女，硕士，主要从事卵菌与真菌分子遗传的研究。E-mail: 2015102039@njau.edu.cn。
通讯作者：郑小波（1957-），男，教授，主要从事卵菌与真菌分子遗传的研究。E-mail: xbzheng@njau.edu.cn。

更新日期/Last Update: 2017-08-14