[1]林洋,刘再胜,单春乔,等.大豆小肽螯合铁的制备工艺及光谱分析研究[J].大豆科学,2017,36(01):108-115.[doi:10.11861/j.issn.1000-9841.2017.01.0108]
　LIN Yang,LIU Zai-sheng,SHAN Chun-qiao,et al.Study on the Preparation Process and Spectroscopic Analysis of Soybean Small-peptide Chelate Iron[J].Soybean Science,2017,36(01):108-115.[doi:10.11861/j.issn.1000-9841.2017.01.0108]
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大豆小肽螯合铁的制备工艺及光谱分析研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年01期 页码: 108-115 栏目: 出版日期: 2017-01-20
Title:
Study on the Preparation Process and Spectroscopic Analysis of Soybean Small-peptide Chelate Iron
作者:
林洋1; 刘再胜2; 单春乔1; 修立颖1; 刘秋晨1; 倪天赫1; 刘艳1; 江国托1
（1.大连三仪动物药品有限公司，辽宁 大连 116036； 2.辽宁省畜产品安全监察所，辽宁 沈阳 110003）
Author(s):
LIN Yang1;  LIU Zai-sheng2;  SHAN Chun-qiao1;  XIU Li-ying1 ; LIU Qiu-chen1 ;  NI Tian-he1;  LIU Yan1;  JIANG Guo-tuo1
（1.Dalian Sanyi Animal Pharmaceutical Co.,LTD, Dalian 116036, China; 2.Animal Product Safety Monitoring Institute of Liaoning Province，Shenyang 110003, China）
关键词:
大豆小肽; 螯合反应; 小肽螯合铁; 有机微量元素; 制备工艺
Keywords:
Soybean small-peptides;  Chelate reaction;  Small-peptide chelate iron;  Organic microelement;  Preparation process
DOI:
10.11861/j.issn.1000-9841.2017.01.0108
文献标志码:
A
摘要:
通过铁源筛选比较得知，氯化亚铁比较适合与大豆小肽进行螯合反应制备大豆小肽螯合铁，利用响应面法优化了大豆小肽螯合铁的制备工艺，优化结果为：小肽与亚铁盐质量比4∶1，反应pH5.0，反应温度40℃，得到离子螯合率平均值为56.81%，经中试车间生产试制得到大豆小肽螯合铁的得率是78.3%，螯合率为82.39%，检测大豆小肽螯合铁的主要成分中的蛋白含量为78.94%，铁的含量为10.87%。红外和紫外光谱分析检测结果显示：大豆小肽和大豆小肽螯合铁（Fe2+）红外吸收峰的强度在不同波长位置上有明显的变化，大豆小肽螯合铁（Fe2+）在紫外波长上发生了明显的位移且宽化，表明大豆小肽螯合铁（Fe2+）形成了络合物。同时对大豆小肽螯合铁的结构进行了预测。
Abstract:
The results from the comparison of iron source selection were showed that ferrous chloride was more suitable for the preparation of soybean peptide chelate iron. According to the study of the optimal process for soybean piptides chelate with different kinds of iron source by using the response surface method. The optimum condition was that mass ratio of small-peptide and ferrous salt was 4∶1, reaction pH was 5.0, reaction temperature was 40℃ and ion average chelating rate reached to 56.81%. In the pilot of workshop manufacturing, the soybean small-peptide chelated iron’s yield was 78.3%, chelating rate was 82.39%, the soybean protein content of small peptide chelate iron sample was 78.94%, iron content was 10.87%. The analysis test by infrared spectroscopic (IR) and Ultra Voilet Spectroscopy (UV), and the comparison for absorption peak between soybean small-peptides and soybean small-peptide chelated iron showed that there was obviously change in absorption in different IR wavelength infrared weak position, and the small soybean peptide chelate iron samples (Fe2+) also changed obviously in the UV wavelength displacement, so it was proved that small soybean peptide chelate iron(Fe2+) had been in complex compound. And its construction was forecast.

参考文献/References:

［1］Parkes J G, Randell E W, Olivieri N F, et al. Modulation by iron loading and chelation of the uptake of non-transferrin-bound iron by human liver cells［J］. Biochimica et Biophysica Acta (BBA)General Subjects, 1995, 1243(3):373-380.

［2］周建群，蒋荣成，覃国喜,等．新一代补铁剂-妈蜜铁完全替代仔猪肌注补铁制剂的对比试验［J］．养猪，2009， 33（5）：14-16．(Zhou J Q, Jiang R C, Qin G X, et al.New generation of iron agent-Mama honey entirely replace piglet intramuscular injection iron preparation of contrast test［J］. Swine Production,2009, 33 (5):14-16.)
［3］Wu H H, Liu Z Y, Zhao Y H, et al. Enzymatic preparation and characterization of iron chelating peptides from anchovy (Engraulis japonicus) muscle protein［J］. Food Research International, 2012,48:435-441.
［4］Alexandra K, Konstantina A, Miehael K, et al. Sensory characteristics and iron dialyzability of gluten-free bread fortified with iron［J］. Food Chemistry, 2007,102(1):309-316．
［5］汪学荣，郑炯，阚建全．猪血多肽螯合盐的制备工艺［J］.食品与发酵工业，2010（6）：89-93.(Wang X R, Zheng J, Kan J Q. The preparation methods of pig blood peptide chelate salt［J］. Food and Fermentation Industries, 2010（6）:89-93.)
［6］郑炯,汪学荣,阚建全.猪血多肽螯合铁的制备工艺研究［J］.食品工业科技，2010，32（2）：261-265.(Zheng J, Wang X R, Kan J Q. Study on preparation of porcine blood polypeptide-iron［J］. Science and Technology of Food Industry,2010,32(2):261-265.)
［7］陈升军．米蛋白肽及米蛋白肽铁鳌合物生产工艺研究［D］．南昌：南昌大学，2008．(Chen S J.Research on production technology of Rice protein peptide and rice protein peptide iron rock compound［D］. Nanchang:Nanchang University,2008.)
［8］吴茹怡，曾里，曾凡骏．复合氨基酸螯合物鉴定方法的研究［J］．食品科学，2006（3）：104-107．(Wu R Y, Zeng L, Zeng F J. Study on identification of compound amino acid chelates［J］.Food Science and Technology, 2006(3):104-107.)
［9］贾玉珠，石邱毅，赵瑛．二硫腙试剂对二硫腙比色法测铅的干扰分析［J］．内蒙古预防医学，1997,24(1):23．(Jia Y Z, Shi Q Y, Zhao Y. Disulfide hydrazone reagent of disulfide hydrazone colorimetric method measuring interference analysis of lead［J］. Neimenggu Preventive Medicine, 1997,24(1):23.)
［10］周建群，罗玉芳，谢梅东．蛋氨酸螯合锌测定方法的研究［J］．新饲料，2007(10)：38-39．(Zhou J Q, Luo Y F, Xie M D. Study of determination method of zinc methionine chelate［J］. Feed Research,2007(10)：38-39.)
［11］李公斌，王振宇．黑木耳多糖与Fe(Ⅱ)鳌合物的制备工艺及红外光谱研究［J］．食品工业科技，2006(5)：136-137.(Li G B,Wang Z Y. Preparing technology of Chelate of Auricularia polysaccharides with Fe(Ⅱ)and study on their IR Spectrum［J］. Science and Technology of Food Industry, 2006(5):136-137.)
［12］李群，刘飞飞，于岚．L赖氨酸锌螯合物的研究［J］．应用化工，2010，39(3)：441-446．( Li Q,Liu F F,Yu L. Progress in study of zinc L lysinate chelate［J］. Applied Chemical Industry, 2010,39(3):441-446.)
［13］黄汉良，凌灵．蛋氨酸锌合成研究［J］．化工技术与开发，2007，36(10)：15-17.( Huang H L, Ling L. Synthesis of zinc methionine complex［J］ Technology & Development of Chemical Industry, 2007, 36(10):15-17.)
［14］吴有炜．试验设计与数据处理［M］．苏州：苏州大学出版社，2002：135-142．( Wu Y W. Experimental design and data processing［M］.Suzhou: Suzhou University Press,2002：135-142.)
［15］崔凤杰，许涨瑜，舒畅,等．响应曲面法优化灰树花水溶性多糖提取工艺的研究［J］．食品科学，2006,27(4):142-151.(Cui F J, Xu Z Y, Shu C, et al. Optmization of processing parameters for extraction of soluble grifola frondosa polysaccharides by response surface methodology［J］. Food Science,2006,27(4):142-151.)

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备注/Memo

第一作者简介：林洋（1984-），男，硕士，工程师，主要从事食品安全与加工研究。E-mail:linyang1984@yeah.net。

通讯作者：江国托（1966-），男，教授，博导，主要从事动物药品研发。E-mail： jgt600@sina.com。

更新日期/Last Update: 2017-03-15