[1]尹学哲,金延华,何鑫,等.大豆异黄酮对过氧化氢诱导的肝细胞损伤的保护作用[J].大豆科学,2016,35(04):687-690.[doi:10.11861/j.issn.1000-9841.2016.04.0687]
　YIN Xue-zhe,JIN Yan-hua,HE Xin,et al.Protective Effect of Soy Isoflavones on Oxidative Damage of Liver Cells Inducedby Hydrogen Peroxide[J].Soybean Science,2016,35(04):687-690.[doi:10.11861/j.issn.1000-9841.2016.04.0687]
点击复制

大豆异黄酮对过氧化氢诱导的肝细胞损伤的保护作用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 687-690 栏目: 出版日期: 2016-07-20
Title:
Protective Effect of Soy Isoflavones on Oxidative Damage of Liver Cells Inducedby Hydrogen Peroxide
作者:
尹学哲1; 金延华1; 何鑫2; 金海南2; 全吉淑2
( 1. 延边大学附属医院，吉林延吉133000; 2. 延边大学医学院，吉林延吉133000)
Author(s):
YIN Xue-zhe1; JIN Yan-hua1; HE Xin2; JIN Hai-nan2; QUAN Ji-shu2
1. Yanbian University Hospital，Yanji 133002，China; 2. Medical College of Yanbian University，Yanji 133000，China
关键词:
大豆异黄酮;  H2O2;  氧化损伤;  肝细胞
Keywords:
Soy isoflavones;  H2O2;  Oxidative damage;  Liver cells
分类号:
Ｒ285. 5
DOI:
10.11861/j.issn.1000-9841.2016.04.0687
文献标志码:
A
摘要:
为探索大豆异黄酮对过氧化氢( H2O2) 致肝细胞损伤的保护作用，以H2O2损伤Chang Liver 细胞建立肝细胞氧化应激损伤模型，并以10， 20 和40 mg·L － 1 大豆异黄酮进行干预。采用四甲基偶氮唑盐比色( MTT) 法检测肝细胞存活率; 以微板法检测细胞培养液中乳酸脱氢酶( LDH) 、谷丙转氨酶( ALT) 、谷草转氨酶( AST) 活性和肝细胞超氧化物歧化酶( SOD) 活性，以及肝细胞还原型谷胱甘肽( GSH) 和丙二醛( MDA) 含量; 采用蛋白印迹技术检测肝细胞核因子-E2 相关因子2( Nrf2) 蛋白含量。在10 ～ 40 mg·L － 1 范围内，大豆异黄酮对Chang Liver 细胞不显示细胞毒作用。300 μmol·L － 1 H2O2刺激后，模型组肝细胞存活率与正常组比较下降，细胞外液中ALT、AST、LDH 水平上升，说明Chang Liver 细胞损伤显著; 而模型组肝细胞中SOD 和GSH 水平与正常组比较下降，丙二醛水平上升，说明模型组Chang Liver 细胞氧化应激增强。大豆异黄酮可剂量依赖性地提高Chang Liver 细胞存活率，降低ALT、AST、LDH 向细胞外液的释放; 降低细胞MDA 水平，升高细胞SOD 和GSH 水平，增高核中Nrf2 蛋白水平。研究结果显示大豆异黄酮对H2O2致肝细胞氧化应激损伤具有保护作用。
Abstract:
The protective effect of soy isoflavones on oxidative damage induced by hydrogen peroxide( H2O2) in liver cells wasinvestigated. Chang Liver cells were exposed to H2O2 and used as the model of oxidative damage. Then the antagonist effect ofsoy isoflavones was detected by pretreatment with 10，20 and 40 mg·L － 1 of soy isoflavones prior to H2O2 challenge. Cell viabilitywas evaluated with MTT assay. The activities of LDH，ALT，AST in culture fluid，and levels of superoxide dismutase( SOD) ，reduced glutathione ( GSH) ，and malondialdelyde( MDA) of liver cells were measured by the microplate tecnique.The protein expression of nuclear factor erythroid2-related factor 2( Nrf2) was determined with the immunoblotting technique.The results showed that 300 μmol·L － 1 H2O2 inhibited cell viabilities，elevated the leakage of LDH，ALT and AST to culturefluid，reduced the levels of SOD and GSH，and increased MDA content in Chang Liver cells. Soy isoflavones did not exert atoxic effect on Chang Liver cells at the concentrations of 10 － 40 mg·L － 1 ，while H2O2 increased oxidative stress and decreasedthe cell viability. Compared with the model group，cell viabilities of soy isoflavone group increased in a dose-dependent manner.Soy isoflavones also reduced the leakage of LDH，ALT and AST to culture fluid，reduced MDA content，and increasedthe levels of SOD and GSH. The administration with soy isoflavones up-regulated the protein expression of Nrf2. Taken together，soy isoflavones have the protective effect on cellular oxidative damage induced by H2O2 in liver cells.

参考文献/References:

［1］ 陶利，李禾. 大豆异黄酮的药理作用［J］. 解放军药学学报，2011，27( 4) : 360-362. ( Tao L，Li H. Pharmacological action ofsoybean isoflavone ［J］. Pharmaceutical Journal of Chinese People’sLiberation Army，2011，27( 4) : 360-362. )

［2］ 尹学哲，赵文玺，金爱花，等. 大豆异黄酮对四氯化碳致小鼠肝脏氧化应激和DNA 损伤的干预作用［J］. 食品科学，2014，35( 1) : 214-218. ( Yin X Z，Zhao W X，Jin A H，et al. Interventioneffect of soy isoflavones on hepatic oxidative stress and dnadamage induced by carbon tetrachloride in mice［J］. Food Science，2014，35( 1) : 214-218. )
［3］ 王力，刘辉，张梦媛，等. 三羟异黄酮对全氟辛酸暴露的小鼠肝脏抗氧化能力的影响［J］. 大豆科学，2014，33 ( 6) : 937-940. ( Wang L，Liu H，Zhang M Y，et al. Effects of genistein onrelevant enzyme activity in liver of mice exposed to perfluorooctanoicacid［J］. Soybean Science，2014，33( 6) : 937-940. )
［4］ Li J F，Chen B C，Lai D D，et al. Soy isoflavone delay the progressionof hepatic fibrosis in thioacetamide-induced model in rats［J］. Scandinavian Journal of Gastroenterology，2011，46 ( 3 ) :341-349.
［5］ 赵育芳，张永生，徐珊，等. 大豆异黄酮对实验性肝纤维化大鼠肝星状细胞活化的影响［J］. 营养学报，2010，32( 3) : 295-296. ( Zhao Y F，Zhang Y S，Xu S，et al. The inhibitory effect ofsoybean isoflavones on activation of hepatic stellate cell in experimentalhepatic fibrosis rats［J］. Acta Nutrimenta Sinica，2010，32( 3) : 295-296. )
［6］ 李健，韩林，马玉芳，等. 黄芪3 种成分对Chang Liver 细胞氧化应激的抑制作用［J］. 中国中药杂志，2015，40( 2) : 318-323. ( Li J，Han L，Ma Y F，et al. Inhibiting effects of threecomponents of Astragalus membranaceus on oxidative stress inChang Liver cells［J］. China Journal of Chinese Materia Medica，2015，40( 2) : 318-323. )
［7］ 高健美，陆国辉，李艳茹，等. 天麻素通过SIＲT1 /PGC-1α 信号通路抗叔丁基过氧化氢诱导的HL7702 细胞氧化损伤［J］.中药药理与临床，2014，30( 4) : 32-35. ( Gao J M，Lu G H，LiY Ｒ，et al. Effects of gastrodin against tert-butyl hydroperoxide-inducedinjury in HL7702 cells via SIＲT1 /PGC-1α pathway ［J］.Pharmacology and Clinics of Chinese Materia Medica，2014，30( 4) : 32-35. )
［8］ 尹学哲，王玉娇，尹基峰，等. 草苁蓉提取物对HepG2 细胞氧化应激损伤的保护作用［J］. 食品科学，2015，36( 15) : 173-178. ( Yin X Z，Wang Y J，Yin J F，et al. Protective effects ofBoschniakia rossica extract on HepG2 cells with oxidative damage［J］. Food Science，2015，36( 15) : 173-178. )
［9］ 金明，王玉娇，金梅花，等. 两种细胞建立肝细胞氧化损伤模型比较［J］. 中国公共卫生，2015，31( 3) : 324-326. ( Jin M，Wang Y J，Jin M H，et al. Comparative study of liver cell modelswith oxidative damage using two cell lines［J］. Chinese Journal ofPublic Health，2015，31( 3) : 324-326. )
［10］ 张婷. 抗氧化基因在大豆异黄酮抑制内皮细胞氧化应激损伤中的作用及机制研究［D］. 重庆: 第三军医大学，2012.( Zhang T. Mechanisms of activation of antioxidant genes in soyisoflavone-mediated attenuaton in oxidative stress-induced cell injuryin EA. hy 926 cells［D］. Chongqing: The Third MilitaryMedical University，2012. )

相似文献/References:

[1]徐美蓉,李玉芳,董煚,等.大豆异黄酮超声波辅助提取条件优化的研究[J].大豆科学,2013,32(01):102.[doi:10.3969/j.issn.1000-9841.2013.01.024]
　XU Mei-rong,LI Yu-fang,DONG Jiong,et al.Optimization of Soy Isoflavone Extraction Assisted with Ultrasonic[J].Soybean Science,2013,32(04):102.[doi:10.3969/j.issn.1000-9841.2013.01.024]
[2]陈玉胜.大豆异黄酮抗高尿酸血症活性研究初探[J].大豆科学,2013,32(02):279.[doi:10.3969/j.issn.1000-9841.2013.02.032]
　CHEN Yu-sheng.Study on Anti-hyperuricemia Activity of Soybean Isoflavone[J].Soybean Science,2013,32(04):279.[doi:10.3969/j.issn.1000-9841.2013.02.032]
[3]沈丹萍,王小平,秦立强.大豆异黄酮摄入与卵巢癌关系的Meta分析[J].大豆科学,2013,32(06):814.[doi:10.11861/j.issn.1000-9841.2013.06.0814]
　SHEN Dan-ping,WANG Xiao-ping,QIN Li-qiang.Isoflavones Intake and Risk of Ovarian Cancer:A Meta Analysis of Epidemiological Study[J].Soybean Science,2013,32(04):814.[doi:10.11861/j.issn.1000-9841.2013.06.0814]
[4]何恩铭,沈瑞池,王 伟,等.紫外分光光度法测定豆渣提取物中大豆异黄酮含量的研究[J].大豆科学,2013,32(06):818.[doi:10.11861/j.issn.1000-9841.2013.06.0818]
　HE En-ming,SHEN Rui-chi,WANG Wei,et al.Determination of Isoflavones in Extraction of Soybean Dregs Using Ultraviolet Spectrophotometry[J].Soybean Science,2013,32(04):818.[doi:10.11861/j.issn.1000-9841.2013.06.0818]
[5]彭 游,喻国贞,郎少杰,等.新型大豆异黄酮萘磺酸酯前药的设计合成[J].大豆科学,2013,32(06):854.[doi:10.11861/j.issn.1000-9841.2013.06.0854]
　PENG You,YU Guo-zhen,LANG Shao-jie,et al.Design and Synthesis of Novel Genistein Naphthylsulfonate Prodrugs[J].Soybean Science,2013,32(04):854.[doi:10.11861/j.issn.1000-9841.2013.06.0854]
[6]刘少静,王多宁,刁颖博,等.大豆异黄酮纯化工艺研究[J].大豆科学,2013,32(04):535.[doi:10.11861/j.issn.1000-9841.2013.04.0535]
　LIU Shao-jing,WANG Duo-ning,DIAO Ying-bo,et al.Optimization on the Purification Technologies of Soybean Isoflavone[J].Soybean Science,2013,32(04):535.[doi:10.11861/j.issn.1000-9841.2013.04.0535]
[7]尹学哲,金延华,金梅花,等.大豆异黄酮对内毒素及D-氨基半乳糖所致小鼠急性肝损伤的保护作用[J].大豆科学,2013,32(04):544.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
　YIN Xue-zhe,JIN Yan-hua,JIN Mei-hua,et al.Protective Effects of Soy Isoflavones on Acute Liver Injury Induced by Lipopolysaccharide and D-galactosamine in Mice[J].Soybean Science,2013,32(04):544.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
[8]董军奎,葛晓月.大豆异黄酮：一种多功能植物药[J].大豆科学,2013,32(04):565.[doi:10.11861/j.issn.1000-9841.2013.04.0565]
　DONG Jun-kui,GE Xiao-yue.Soy Isoflavone:The Multipurpose Phytopharm[J].Soybean Science,2013,32(04):565.[doi:10.11861/j.issn.1000-9841.2013.04.0565]
[9]李怡然,赵丽芹,李爱科,等.豆浆中水溶性大豆异黄酮检测方法的建立[J].大豆科学,2014,33(03):429.[doi:10.11861/j.issn.1000-9841.2014.03.0429]
　LI Yi-ran,ZHAO Li-qin,LI Ai-ke,et al.Construction of Detection Method for Water soluble Isoflavone Content in Soybean Milk[J].Soybean Science,2014,33(04):429.[doi:10.11861/j.issn.1000-9841.2014.03.0429]
[10]刘中华,胡春红,郭 婕,等.低温豆粕中异黄酮和皂甙的微波同步提取工艺[J].大豆科学,2014,33(03):443.[doi:10.11861/j.issn.1000-9841.2014.03.0443]
　LIU Zhong-hua,HU Chun-hong,GUO Jie,et al.Microwave Extraction of Isoflavones and Saponins in the Low temperature ?Soybean Meal[J].Soybean Science,2014,33(04):443.[doi:10.11861/j.issn.1000-9841.2014.03.0443]

备注/Memo

基金项目: 国家自然科学基金( 81160539) 。第一作者简介: 尹学哲( 1962-) ，男，博士，教授，主要从事分子肿瘤学研究。E-mail: yinxz@ ybu． edu． cn。通讯作者: 全吉淑( 1968-) ，女，博士，教授，主要从事中药药理学研究。E-mail: quanjs@ ybu． edu． cn。

更新日期/Last Update: 2016-08-23