[1]丁福全,杜海龙,索艺宁,等.大豆GmGBP1 启动子受激素和外源环境诱导的研究[J].大豆科学,2016,35(04):683-686.[doi:10.11861/j.issn.1000-9841.2016.04.0683]
　DING Fu-quan,DU Hai-long,SUO Yi-ning,et al.Ｒesearch of the Promoter of Soybean GmGBP1 Induced by Hormone and ExogenousEnvironment[J].Soybean Science,2016,35(04):683-686.[doi:10.11861/j.issn.1000-9841.2016.04.0683]
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大豆GmGBP1 启动子受激素和外源环境诱导的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 683-686 栏目: 出版日期: 2016-07-20
Title:
Ｒesearch of the Promoter of Soybean GmGBP1 Induced by Hormone and ExogenousEnvironment
作者:
丁福全; 杜海龙; 索艺宁; 杨雪; 李敏敏; 张可欣; 赵琳; 李文滨
( 东北农业大学大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江哈尔滨150030)
Author(s):
DING Fu-quan; DU Hai-long; SUO Yi-ning; YANG Xue; LI Min-min; ZHANG Ke-xin; ZHAO Lin; LI Wen-bin
Agricultural College of Northeast Agricultural University /Key Laboratory of Soybean Biology in Chinese Ministry of Education /Key Laboratory of SoybeanBiology and Breeding ＆ Genetics of Chinese Agriculture Ministry，Harbin 150030，China
关键词:
拟南芥;  GmGBP1 启动子;  GUS 染色
Keywords:
Arabidopsis;  GmGBP1 promoter;  GUS staining
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0683
文献标志码:
A
摘要:
采用PCＲ 方法克隆大豆光周期GmGBP1 基因启动子，构建含有GmGBP1 启动子驱动报告基因GUS 的融合性表达载体pBI121-pGmGBP1: : GUS，通过花序浸泡法，转化野生型拟南芥获得pBI121-pGmGBP1: : GUS 转基因植株，对T3代转基因拟南芥进行GUS 染色，研究了GmGBP1 启动子受激素和外源环境诱导的表达规律。结果表明: 大豆GmGBP1启动子受水杨酸( SA) 和干旱( PEG) 以及高温等外源环境的诱导，与生物信息学预测GmGBP1 启动子序列存在的逆境胁迫响应元件的结果相一致。
Abstract:
The expression pattern of the GmGBP1 promoter under hormone and environment were obtained． The GmGBP1 promoterwas cloned by PCＲ and the fusion expression vector was constructed，including the GmGBP1 promoter and the reportergene GUS． The fusion expression vector was used to infect Arabidopsis thaliana，and T3 generation of transgenic Arabidopsiswas obtained，then the GUS staining was used to research the expression pattern of the GmGBP1 promoter in Arabidopsis thaliana．The results showed that the expression of the GmGBP1 promoter was regulated by GA，SA，drought and high temperature，consistent with the predictions of GmGBP1 promoter element by bioinformatics analysis． Our experiment provide theoreticalbasis for the cultivation of resistant crops in the future．

参考文献/References:

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备注/Memo

基金项目: 国家自然科学基金( 31271748) ; 转基因生物新品种培育科技重大专项( 2014ZX08004-005) ; 东北农业大学“青年才俊”项目( 14QC23) ; 黑龙江省自然科学基金( C2015009) ; 东北农业大学2015 年大学生SIPT 计划( 201510224187) ; 东北农业大学2016年大学生SIPT 计划( 201610224141) ; 黑龙江省教育厅青年学术骨干项目( 1253G010) ; 十二五农村领域国家科技计划课题( 2013AA102602) ; 东北农业大学研究生科技创新基金( yjscx14047) 。第一作者简介: 丁福全( 1991-) ，男，硕士，主要从事大豆生物技术研究。E-mail: 409034679@ qq． com。通讯作者: 赵琳( 1980-) ，女，研究员，博导，主要从事大豆生物技术研究。E-mail: zhaolinneau@126． com;李文滨( 1958-) ，男，教授，博导，主要从事大豆生物技术研究。E-mail: wenbinli@ yahoo． com。

更新日期/Last Update: 2016-08-23