HUANG Xia,MA Huan-le,LI Wan-yu,et al.Effect of Photosynthetic Bacteria Producing 5-aminolevulinic Acid( ALA) on theSeveral Index of Seedling Establishment of the Aged Soybean Seed[J].Soybean Science,2016,35(04):626-629.[doi:10.11861/j.issn.1000-9841.2016.04.0626]
产ALA 光合细菌对陈化大豆种子幼苗建成若干指标的影响
- Title:
- Effect of Photosynthetic Bacteria Producing 5-aminolevulinic Acid( ALA) on theSeveral Index of Seedling Establishment of the Aged Soybean Seed
- 关键词:
- 陈化的大豆种子; 光合细菌; 5-氨基乙酰丙酸( ALA) ; 出苗率; 幼苗
- Keywords:
- Aging soybean seeds; Photosynthetic bacteria; 5-aminolevulinic acid; Germination; Seedling
- 分类号:
- S565. 1
- 文献标志码:
- A
- 摘要:
- 为了降低ALA 的生产和应用成本,在实验室条件下评估了产ALA 光合细菌的培养物与低浓度商品ALA 的溶液对陈化1 年的大豆种子出苗和幼苗建成的若干生物学指标的影响。结果表明: 供试产ALA 光合细菌的培养液的上清液( ALA 含量约为0. 1 ~ 0. 2 mg·L - 1 ) 、菌体悬浮液处理对陈化大豆种子萌发后幼苗株高和根系生长有显著促进作用,但对陈大豆种子的出苗率和幼苗下胚轴粗、鲜重、干重没有显著促进作用,而5 mg·L - 1 的ALA 溶液可显著提高陈大豆种子的出苗率、下胚轴粗、幼苗鲜重,1mg·L - 1 ALA 可显著提高幼苗株高、下胚轴粗和干重。光合细菌的分泌物和胞外代谢产物对陈大豆种子萌发后幼苗的株高和根系生长有明显的促进作用。
- Abstract:
- In order to reduce the cost of production and application of 5-aminolevulinic Acid( ALA) ,the effects of culturecontaining 5-aminolevulinic acid( ALA) secreted by strains of photosynthetic bacteria and synthetic ALA( positive control) onthe germination rate of aged soybean seed and the several biology index of soybean seedlings establishment were assessed underlaboratory. The results showed that culture of photosynthetic bacteria in particular the culture supernatant( ALA containing approximately0. 1 - 0. 2 mg·L - 1 ) gave significantly better seedling height and root growth than other treatments,but theydidn’t significantly improve germination rate,seedling hypocotyl diameter,fresh weight and dry weight. 5 mg·L - 1 ALA increasedgermination rate significantly,hypocotyls diameter and seedling fresh weight,and 1 mg·L - 1 ALA increased shootsheight,hypocotyls diameter and dry weight significantly.
参考文献/References:
1] Akram N A,Ashraf M. Regulation in plant stress tolerance by apotential plant growth regulator,5-aminolevulinic acid[J]. Journalof Plant Growth Regulation,2013,32: 663-679.
备注/Memo
基金项目: 2014 年黑龙江八一农垦大学大学生创新创业训练计划项目。第一作者简介: 黄霞( 1994-) ,女,主要从事生物技术研究。E-mail: 1336764422@ qq. com。通讯作者: 崔战利( 1964-) ,女,硕士,教授,主要从事微生物学的教学与研究。E-mail: zhanlic@ aliyun. com。