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[1]王爽,郭兵福,郭勇,等.病毒诱导的基因沉默( VIGS) 技术及其在大豆基因功能研究和育种中的应用潜力[J].大豆科学,2016,35(04):531-535.[doi:10.11861/j.issn.1000-9841.2016.04.0536]
　WANG Shuang,GUO Bing-fu,GUO Yong,et al.Virus-induced Gene Silencing and Its Usage in Soybean Functional Genomics[J].Soybean Science,2016,35(04):531-535.[doi:10.11861/j.issn.1000-9841.2016.04.0536]

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病毒诱导的基因沉默( VIGS) 技术及其在大豆基因功能研究和育种中的应用潜力

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷期数: 2016年04期页码: 531-535 栏目: 出版日期: 2016-07-20

Title:: Virus-induced Gene Silencing and Its Usage in Soybean Functional Genomics

作者:: 王爽¹; 2; 郭兵福²; 郭勇²; 张丽娟²; 金龙国²; 杨慧²; 邱丽娟²; 1. 东北农业大学农学院，黑龙江哈尔滨150030;?
2. 中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程，北京100081

Author(s):: WANG Shuang¹; 2; GUO Bing-fu²; GUO Yong²; ZHANG Li-juan²; JIN Long-guo²; YANG Hui²; QIU Li-juan²; 1. College of Agriculture，Northeast Agricultural University，Harbin 150030，China; 2. The National Key Facility for Crop Gene Ｒesources and GeneticImprovement ( NFCＲI) / MOA Key Lab of Soybean Biology ( Beijing) ， Institute of Crop Science，Chinese Academy of Agricultural Sciences，Beijing100081，China

关键词:: 病毒诱导的基因沉默; VIGS; 病毒载体; 功能基因; 育种

Keywords:: Virus － induced gene silencing; VIGS; Virus vector; Functional gene; Breeding

分类号:: S565. 1

DOI:: 10.11861/j.issn.1000-9841.2016.04.0536

文献标志码:: A

摘要:: 病毒诱导的基因沉默( virus-induced gene silencing，VIGS) 是植物体抵抗病毒侵染的一种自然防御机制，近年来已发展成为一种基因功能研究的有效手段。与突变体筛选、转基因、基因敲除等技术相比，VIGS 技术因具有周期短、成本低、可迅速观察表型等优点而被广泛应用于基因的功能鉴定。文章对VIGS 技术的分子机理、病毒载体系统、稳定遗传VIGS 植株的获得策略及其在大豆中的应用等内容进行了综述并对今后的发展趋势进行了讨论。

Abstract:: Virus-induced gene silencing is the innate plant defense system against intracellular viral proliferation and extracellularviral movement. VIGS is nowadays widely used in plant genetics for gene knockdown due to its ease of use and the shorttime required to generating phenotypes. In this paper，the molecular mechanism of VIGS and the VIGS virus vector system wasreviewed. The strategy to obtain stable VIGS plants and the application of VIGS has also been discussed.

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相似文献/References:

[1]黄赛花,郑桂杰,杨永庆,等.利用VIGS技术对抗SMV候选基因GmZ-15的功能分析[J].大豆科学,2015,34(04):582.[doi:10.11861/j.issn.1000-9841.2015.04.0582]
　HUANG Sai-hua,ZHENG Gui-jie,YANG Yong-qing,et al.Analysis on the Candidate Resistance Gene GmZ15 to Soybean Mosaic Virus by VIGS[J].Soybean Science,2015,34(04):582.[doi:10.11861/j.issn.1000-9841.2015.04.0582]

备注/Memo

基金项目: 转基因新品种培育重大专项( 2014ZX08004-001) 。第一作者简介: 王爽( 1990-) ，女，硕士，主要从事大豆转基因研究。E-mail: 675659474@ qq. com。通讯作者: 邱丽娟( 1963-) ，女，研究员，博导，主要从事大豆基因资源挖掘与利用工作。E-mail: qiulijuan@ caas. cn。

更新日期/Last Update: 2016-08-22