[1]石云,孔祥珍,华欲飞.离子交换树脂纯化大豆糖蜜上清液[J].大豆科学,2016,35(01):130-135.[doi:10.11861/j.issn.1000-9841.2016.01.0130]
　SHI Yun,KONG Xiang-zhen,HUA Yu-fei.Purification of Soy Molasses Supernatant by Ion-Exchange Resins[J].Soybean Science,2016,35(01):130-135.[doi:10.11861/j.issn.1000-9841.2016.01.0130]
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离子交换树脂纯化大豆糖蜜上清液

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年01期 页码: 130-135 栏目: 出版日期: 2016-01-20
Title:
Purification of Soy Molasses Supernatant by Ion-Exchange Resins
作者:
石云; 孔祥珍; 华欲飞
江南大学 食品学院/食品科学与技术国家重点实验室，江苏 无锡 214122
Author(s):
SHI Yun;  KONG Xiang-zhen;  HUA Yu-fei
School of Food Science and Technology/State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
关键词:
大豆糖蜜; 离子交换; 大豆低聚糖; 吸附
Keywords:
Soy molasses;  Ion-exchange;  Soy oligosaccharides;  Adsorption
DOI:
10.11861/j.issn.1000-9841.2016.01.0130
文献标志码:
A
摘要:
大豆糖蜜上清液中除大豆低聚糖（蔗糖、棉子糖和水苏糖）外，还含有色素、无机盐、含氮组分和有机酸等杂质。本文主要研究了离子交换树脂纯化脱色糖蜜上清液过程中去除杂质的机理并优化了纯化工艺。结果显示：脱色上清液中主要的含氮组分有5种游离氨基酸（天冬氨酸、谷氨酸、天冬酰胺、丙氨酸、酪氨酸）和1种二肽（谷氨酸-酪氨酸）。用离子交换树脂直接处理脱色上清液的效率很低，含氮组分在3倍柱体积处理量时穿透；而离子交换树脂对纯含氮组分的吸附能力很强，处理量约为40倍柱体积。因此，无机盐（Na+、K+和Cl-）和有机酸（柠檬酸）成为了树脂吸附含氮组分的制约因素。用电渗析法脱除无机盐后，阳离子交换树脂对含氮组分的吸附效果显著改善，处理量升高到35倍柱体积；继续用阴离子交换树脂吸附阳离子树脂流出液中的有机酸，即可获得高纯度的大豆低聚糖。同时通过拟合Thomas方程，分别计算了阳离子交换树脂吸附含氮组分和阴离子交换树脂吸附柠檬酸的最大固相吸附浓度。
Abstract:
Soy molasses supernatant mainly consists of soy oligosaccharides (sucrose, raffinose and stachyose) and other impurities such as chromogenic substances, inorganic salts, nitrogen-containing components and organic acids.This paper studied the mechanism of removing impurities from decolorized soy molasses supernatant by ion-exchange resins, and optimized the process.The results showed that the main nitrogen-containing components were five kinds of free amino acids (Aspartic acid, Glutamic acid, Asparagine, Alanine and Tyrosine) and one kind of dipeptide (Glu-Tyr).Adoption of decolorized supernatant by ion-exchange resins showed low efficient since the handling capacity was about just 3 times of column volume when the effluent broke through. However, ion-exchange resins performed very well on adsorbing nitrogen-135containing components from pure solution with handling capacity of 40 times of column volume. Therefore, it was reasonable to consider that the limiting factors of adsorption were inorganic salts (Na+, K+and Cl-) and organic acid (Citric acid). After desalination by electrodialysis, handling capacity for adsorption of nitrogen-containing components by anion resin rose to about 35 times of column volume.And then high-purity soy oligosaccharides could be got by removing organic acid using anion resin from effluent of cation resin column Besides, the maximum solid phase concentration for nitrogen-containing components and citric acid was respectively calculated by fitting Thomas model.

参考文献/References:

［1］Lan Y, Williams B A, Verstegen M W A. Soy oligosaccharides in vitro fermentation characteristics and its effect on caecal microorganisms of young broiler chickens ［J］. Animal Feed Science and Technology, 2007, 133: 286-297.

［2］蒋丽华.大豆糖蜜中低聚糖的分离纯化［D］. 无锡：江南大学, 2007: 32-36.（Jiang L H. Separation and purification of oligosaccharides from soy molasses ［D］. Wuxi: Jiangnan University, 2007: 32-36.）
［3］马永香，华欲飞，孔祥珍，等.离子交换树脂对大豆糖蜜上清液脱盐脱色工艺的研究［J］ 中国油脂, 2013, 38(1): 70-75. (Ma Y X, Hua Y F, Kong X Z, et al. Effect of ion exchange resin on desalination and decoloration of supernatant of soybean molasses ［J］ China Oils and Fats, 2013, 38(1): 70-75.)
［4］谷春梅，韩玲玲，曲洪生，等.大豆胰蛋白酶抑制因子的分离纯化［J］ 大豆科学, 2013, 32(1): 89-92. (Gu C M, Han L L, Qu H S, et al. Extraction and purification of soybean trypsin inhibitor ［J］. Soybean Science, 2013, 32(1): 89-92.)
［5］董婷，刘会平，胡赞扬. 乳清中乳碱性蛋白的分离与纯化［J］.食品科学, 2010,31(14): 62-66. (Dong T, Liu H P, Hu Z Y. Isolation and purification of milk basic protein from cheese whey ［J］. Chinese Food Science, 2010, 31(14): 62-66.)
［6］易馨如，封棣，王泰，等. 离子交换树脂对藤黄果中羟基柠檬酸的分离纯化［J］. 食品与机械, 2014, 30(3): 179-184.(Yi X R, Feng D, Wang T, et al.Separation and purification of hydroxycitric acid from Garcinia cambogia with ion exchange resin ［J］.Food & Machinery, 2014, 30(3): 179-184.)
［7］胡靖，丘树毅，吴海，等. 离子交换法吸附分离黄水中的有机酸［J］. 酿酒科技, 2012 (5): 24-26.(Hu J, Qiu S Y, Wu H, et al. Separation and adsorption of organic acids from yellow water by ion exchange resins ［J］ Liquor-Making Science & Technology, 2012(5): 24-26.)
［8］赵华，王海涛，董银卯，等. 离子交换树脂对米邦塔仙人掌粉提取液的脱盐脱色［J］.食品工业科技, 2010, 31(7): 213-219. (Zhao H, Wang H T, Dong Y M, et al. Desalination and decolorization of Opunitia Milpa Alta powder extract by ion exchange resins ［J］. Science and Technology of Food Industry, 2010, 31(7): 213-219.)
［9］盛金凤，李丽，孙健. 蔗渣制备低聚木糖溶液的脱色脱盐工艺及其组分分析［J］. 食品科学, 2014, 35(14): 40-45. (Sheng J F, Li L, Sun J. Decolorization, demineralization and monosaccharide composition of xylooligosaccharides from sugarcane bagasse ［J］. Chinese Food Science, 2014, 35(14): 40-45.)
［10］田亚平. 生化分离技术［M］.北京: 化学工业出版社, 2006: 99-101.(Tian Y P. Biochemical separation technology ［M］. Beijing: Chemical Industry Press, 2006: 99-101.)
［11］潘红阳，王树英，江波，等. OPA柱前自动衍生HPLC-FLD法测定玉米低聚肽中AY的含量［J］. 食品与发酵工业, 2012, 38(6): 182-185. (Pan H Y, Wang S Y, Jiang B, et al.Determination of AY in corn oligopeptides by High-performance Liquid Chromatography-Fluorescence Detector with OPA precolumn derivatization ［J］. Food and Fermentation Industries, 2012, 38(6): 182-185.)
［12］马永香. 利用吸附、包埋及离子交换法制备大豆低聚糖浓缩糖浆的研究［D］.无锡：江南大学, 2013: 16.（Ma Y X.Study on the preparation of soybean oligosaccharide syrup by adsorption, inclusion and ion exchange ［D］. Wuxi: Jiangnan University, 2013: 16.）
［13］章绍兵，石云，王璋，等. 离子交换色谱和凝胶过滤分离纯化菜籽抗氧化肽［J］.中国粮油学报, 2008, 23(5): 154-159. (Zhang S B, Shi Y, Wang Z, et al. Purification of rapeseed antioxidant peptides by ion exchange and gel filtration chromatograph ［J］.Journal of the Chinese Cereals and Oils Association, 2008, 23(5): 154-159.)
［14］钟芳，张晓梅，麻建国.大豆肽的离子交换色谱分离及其活性评价［J］. 食品与机械, 2006, 22(5): 16-19.(Zhong F, Zhang X M, Ma J G. Isolation and identification of ACE inhibition peptides from soy hydrolytes with ion-exchange chromatograph ［J］. Food & Machinery, 2006, 22(5): 16-19.)?
［15］郑辉杰，陈洵，邸进申，等. 离子交换树脂对海藻糖乙醇提取液脱盐脱色的研究［J］.河北工业大学学报, 2008, 37(5): 37-43. (Zheng H J, Chen X, Di J S, et al. The study of demineralization and decolorization of trehalose ethanol extract by ion exchange resin ［J］.Journal of Hebei University of Technology, 2008, 37(5): 37-43.)?
［16］Vijayaraghavan K, Jegan J, Palanivelu K, et al.Batch and column removal of copper from aqueous solutionusing a brown marine alga Turbinaria ornate［J］. Chemical Engineering Journal, 2005, 106: 177-184.
［17］Han R P, Ding D D, Xu Y F, et al.Use of rice husk for the adsorption of congo red from aqueous solution in column mode ［J］. Bioresource Technology, 2008, 99: 2938-2946.
［18］Zümriye A, Ferda G.Biosorption of phenol by immobilized activated sludge in a continuous packed bed: prediction of breankthroug curves ［J］. Process Biochemistry, 2004, 39: 599-613.

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备注/Memo

基金项目：国家高技术发展研究计划（“863计划”）（2013AA102200）。

第一作者简介：石云（1984-），女，博士，主要从事大豆糖蜜相关研究。E-mail：shiyun0301@163.com。
通讯作者：华欲飞(1962-)，男，博士，教授，主要从事植物蛋白相关研究。E-mail：yfhua@jiangnan.edu.cn。

更新日期/Last Update: 2016-02-05