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[1]张瑞萍,王家军,魏崃,等.一株大豆腐霉菌(PSHH-1)rDNA的ITS序列鉴定[J].大豆科学,2015,34(05):867-873.[doi:10.11861/j.issn.1000-9841.2015.05.0867]
　ZHAN Grui-ping,WANG Jia-jun,WEI Lai,et al.Internal Transcribed Spacer(ITS) sequence identification of rDNA in a Pythium strain PSHH1[J].Soybean Science,2015,34(05):867-873.[doi:10.11861/j.issn.1000-9841.2015.05.0867]

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一株大豆腐霉菌(PSHH-1)rDNA的ITS序列鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷期数: 2015年05期页码: 867-873 栏目: 出版日期: 2015-10-25

Title:: Internal Transcribed Spacer(ITS) sequence identification of rDNA in a Pythium strain PSHH1

作者:: 张瑞萍¹; 王家军¹; 魏崃¹; 张武²; 栾晓燕¹; 唐晓飞¹; 刘鑫磊¹; 1.黑龙江省农业科学院大豆研究所, 黑龙江哈尔滨 150086；
2.黑龙江省农业科学院黑河分院, 黑龙江黑河 16430

Author(s):: ZHAN Grui-ping¹; WANG Jia-jun¹; WEI Lai¹; ZHANG Wu²; LUAN Xiao-yan¹; TANG Xiao-fei¹; LIU Xin-lei¹; 1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;
2.Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, China

关键词:: 腐霉菌; ITS序列鉴定; 致病力

Keywords:: Pythium; Internal Transcribed Spacer(ITS) sequence identification; Pathogenicity

DOI:: 10.11861/j.issn.1000-9841.2015.05.0867

文献标志码:: A

摘要:: 大豆腐霉菌是黑龙江省大豆根腐病菌群的重要组成部分，为了明确大豆腐霉菌的种类，从黑龙江省黑河市大豆根系土壤分离获得1株腐霉菌PSHH1，应用Rdna-ITS序列分析法对其进行菌种鉴定。Blast和系统进化树结果表明：PSHH1与GenBank上登录号为AB259316.1和AB512973.1等的Pythium sylvaticum亲缘关系最近，rDNA-ITS序列同源性大于99%，因此认定PSHH1为Pythium sylvaticum。为了考察PSHH1的致病性，选取了60份当前大豆主栽品种，应用种子腐烂法和下胚轴接种法进行回接试验，通过计算种子腐烂率和植株茎折率来评价PSHH1的致病性，结果显示，PSHH1仅对13.33%的大豆主栽品种表现出较强的致病力，而对6167%的大豆主栽品种致病力较弱。

Abstract:: Pythium is an important part of soybean root rot fungal population in Heilongjiang province, in order to determine the species of Pythium in Heilongjiang province, PSHH1 has been isolated from the soil of soybean root in Heihe city. And Rdna-ITS sequence analysis was applied to PSHH1。According to the results of the analysis of rDNA-ITS sequences, the sequence homology was more than 99% when it was compared with the sequence of registration number AB259316.1 and AB512973.1 in GeneBank.Therefore, we think PSHH1 was a Pythium sylvaticum. In order to study the pathogenicity of PSHH1, using seed rot method and hypocotyl inoculation method, 60 main soybean cultivars were selected to determine the pathogenicity of PSHH1, and seed rot rate and stem discount rate can be reflected to evaluate the pathogenicity of PSHH1,results showed that PSHH1 had strong pathogenicity to 13.3% main soybean cultivars, at the same time, there were 61.67% main soybean varieties expressed weak pathogenicity to PSHH1。

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相似文献/References:

[1]魏崃,薛永国,王伟威,等.应用关联分析鉴定大豆对腐霉菌的抗性基因[J].大豆科学,2013,32(03):295.[doi:10.11861/j.issn.1000-9841.2013.03.0295]
　WEI Lai,XUE Yong-guo,WANG Wei-wei,et al.Identification of Resistance Genes to Pythium Species in Soybeans by Association Analysis[J].Soybean Science,2013,32(05):295.[doi:10.11861/j.issn.1000-9841.2013.03.0295]
[2]于洪久,朱洪德,刘丽君,等.腐霉菌侵染条件下大豆下胚轴中PAL和POD活性的变化[J].大豆科学,2012,31(03):495.[doi:10.3969/j.issn.1000-9841.2012.03.034]
　YU Hong-jiu,ZHU Hong-de,LIU Li-jun,et al.PAL and POD Activities of Soybean Hypocotyls Infected by Pythium [J].Soybean Science,2012,31(05):495.[doi:10.3969/j.issn.1000-9841.2012.03.034]
[3]魏崃,李文滨,韩英鹏,等.大豆品种对四种腐霉菌的抗性研究[J].大豆科学,2010,29(06):971.[doi:10.11861/j.issn.1000-9841.2010.06.0971]
　WEI Lai,LI Wen-bin,HAN Ying-peng,et al.Identification of Resistance to Four Pythium Species in Soybean Cultivars[J].Soybean Science,2010,29(05):971.[doi:10.11861/j.issn.1000-9841.2010.06.0971]

备注/Memo

基金项目：黑龙江省农业科技创新工程(2012ZD004)；哈尔滨市应用技术研究与开发项目(2013RFXYJ082)；国家现代农业产业技术体系(CARS-04-PS05)。第一作者简介：张瑞萍（1979-），女，博士，副研究员，主要从事作物病理学研究。E-mail：ruiping_zhang121@126.com。

通讯作者：王家军（1973.），男，硕士，副研究员，主要从事大豆植物保护研究。E-mail：junjiawang@163.com。

更新日期/Last Update: 2015-11-08