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[1]王大刚,等.大豆花叶病毒致病基因的克隆与序列分析[J].大豆科学,2015,34(05):760-767.[doi:10.11861/j.issn.1000-9841.2015.05.0760]
　WANG Da-gang,ZHI Hai-jian,et al.Clone and Sequence Analysis of Virulence Genes of Soybean Mosaic Virus Isolates in China[J].Soybean Science,2015,34(05):760-767.[doi:10.11861/j.issn.1000-9841.2015.05.0760]

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大豆花叶病毒致病基因的克隆与序列分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷期数: 2015年05期页码: 760-767 栏目: 出版日期: 2015-10-25

Title:: Clone and Sequence Analysis of Virulence Genes of Soybean Mosaic Virus Isolates in China

作者:: 王大刚¹; 2; 智海剑²; 田震²; 黄志平¹; 吴倩¹; 张磊¹; 1.安徽省农业科学院作物研究所/安徽省农作物品质改良重点实验室，安徽合肥 230031；
2.南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏南京 210095

Author(s):: WANG Da-gang¹; 2; ZHI Hai-jian²; TIAN Zhen²; HUANG Zhi-ping¹; WU Qian¹; ZHANG Lei¹; 1.Crop Institute of Anhui Academy of Agricultural Sciences/Key Laboratory of Crop Quality Improvement of Anhui Province, Hefei 230031, China;
2.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement,Nanjing 210095, China

关键词:: 大豆花叶病毒; 致病基因; 克隆; 序列分析

Keywords:: Soybean mosaic virus; Virulent genes; Clone; Sequences analysis

DOI:: 10.11861/j.issn.1000-9841.2015.05.0760

文献标志码:: A

摘要:: 通过生物学纯化与血清学鉴定（ELISA）得到5个大豆花叶病毒(SMV)分离物，利用RT-PCR法扩增其CP、HC-Pro、P1和P3基因片段并进行测序。结果表明：5个分离物P1基因全长均为927个核苷酸，编码产生309个氨基酸。同源性分析表明，5个分离物核苷酸序列同源性为88.0%~99.9%，由此推导的氨基酸序列同源性为86.1%~100.0%。此外，5个分离物4个SMV基因CP、HC-Pro、P1和P3长度均为4 137个核苷酸，编码1 378个氨基酸。分析结果显示，5个分离物之间的核苷酸及氨基酸的同源性分别为92.6%~99.3%和95.1%~99.2%。根据系统进化树的分析，结合5个分离物在10个大豆鉴别寄主上的致病性反应，发现SMV的4个基因CP、HC-Pro、P1和P3与SMV的致病力及病样的来源地之间具有一定的关系。同时，这4个SMV基因能够将传统的SMV株系分离物与重组性分离物进行区分。

Abstract:: Five SMV isolates were obtained by using biological purification and serology (DAS-ELISA).The CP,HC-Pro,P1 and P3 gene regions were amplified by reverse transcription polymerase chain reaction (RT-PCR)Sequences analysis showed that P1 gene of the each SMV isolates had 927 nucleotides in length, encoding 309 amino acids.Comparison of the sequences of P1 genes between different isolates showed they shared 88.0%-99.9% nucleotide acids identities and 86.1%-100.0% amino acids identities with each other. The four genes from the 5 SMV isolates were composed of 4 137 nucleotides, encoding 1 378amino acids, and shared 92.6%-99.3% nucleotide sequence identities and 95.1%-99.2% amino acid sequence identities. The results also revealed that the sequences variation in the CP,HC-Pro,P1 and P3 genes of virus were correlated with the level of virulence and the source of the virus isolates based on the symptom reaction of SMV on differential hosts and the phylogenetic analyses .Meanwhile, the normal soybean mosaic virus isolates and recombinant isolates could be differentiated through the sequences variation of CP,HC-Pro,P1 and P3genes.

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备注/Memo

基金项目：国家自然科学基金（31201235, 31371646, 31401402）；安徽省自然科学基金（1308085QC59）；国家现代农业产业技术体系建设专项（CARS-04）；南京农业大学作物遗传与种质创新国家重点实验室开放课题（ZW2013）。

第一作者简介：王大刚（1979-），男，博士，副研究员，主要从事大豆抗病遗传育种。E-mail: smvwang@163.com。

通讯作者：张磊（1956-），男，研究员，主要从事大豆育种研究。E-mail: leizh66@163.com。

更新日期/Last Update: 2015-11-07