[1]鲁建聪,宋洁,许艳丽.真菌发酵滤液对大豆胞囊线虫二龄幼虫趋性的影响[J].大豆科学,2015,34(04):671-674,678.[doi:10.11861/j.issn.1000-9841.2015.04.0671]
　LU Jian-cong,SONG Jie,XU Yan-li.Effects of Parasitic Fungal Fermentation Filtrate on Chemotaxis of Soybean Cyst Nematode Juvenile[J].Soybean Science,2015,34(04):671-674,678.[doi:10.11861/j.issn.1000-9841.2015.04.0671]
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真菌发酵滤液对大豆胞囊线虫二龄幼虫趋性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 671-674,678 栏目: 出版日期: 2015-08-25
Title:
Effects of Parasitic Fungal Fermentation Filtrate on Chemotaxis of Soybean Cyst Nematode Juvenile
作者:
鲁建聪1; 2; 宋洁1; 2; 许艳丽2
1.东北农业大学 农学院，黑龙江 哈尔滨 150030；
2.中国科学院 东北地理与农业生态研究所/黑土区农业生态院重点实验室，黑龙江 哈尔滨 150081
Author(s):
LU Jian-cong1; 2;  SONG Jie1; 2;  XU Yan-li2
1.College of Agronomy, Northeast Agriculture University, Harbin 150040, China;?
2.Key Laboratory of Mollisols Agroecology/Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China
关键词:
大豆胞囊线虫二龄幼虫; 寄生真菌; 趋性; 发酵滤液; 根浸提液
Keywords:
Soybean cyst nematode juvenile (J2);  Parasitic fungal;  Chemotaxis;  Fermentation filtrate;  Soybean root diffusate
DOI:
10.11861/j.issn.1000-9841.2015.04.0671
文献标志码:
A
摘要:
采用滤纸片法和直接法，通过考察大豆胞囊线虫二龄幼虫（J2）在WA平板上不同区域内的分布率，研究淡紫拟青霉（Paecilomyces lilacinus）、镰孢菌属（Fusarium spp）和厚垣轮枝菌(Pochonia chlamydosporium)的12个不同菌株代谢物滤液对大豆胞囊线虫J2趋性的影响。滤纸片法结果显示，WA培养基上0~1 cm区间内，大豆胞囊线虫二龄幼虫（J2）在12个寄生真菌菌株代谢物滤液中分布率均较低， F9-3、V2、F5和V1菌株代谢物滤液中的分布率分别为17.3%、17.3%、18.0%和18.3%，显著低于对照水的分布率26.0%，也低于J2在根系分泌物中的分布率32.3% (P＜005)。直接法结果与滤纸片法基本一致，大豆幼根蘸取寄生真菌代谢物滤液后，线虫幼虫在0~1 cm区间内分布率范围为18.0%~31.3%，显著低于幼根蘸水对照（P＜0.05），说明大豆胞囊线虫J2对12个寄生真菌菌株代谢物滤液存在一定的趋避性。
Abstract:
The soybean cyst nematode (SCN) is one of the serious diseases in soybean production. With the current emphasis on environmental pollution and the demand for pollution-free agricultural products, nematodes biological control research received more attention. The effect of 12 parasitic fungal isolates filtrates which were made by Paecilomyces lilacinus, Fusarium spp Symbol`@@and Pochonia chlamydosporium was tested on SCN. It was investigated by investigated the difference of J2 distribution rate on WA.The methods of filtering paper and direct test were conducted in our laboratory.Both methods showed that SCN J2 were repelled by 12 parasitic fungal isolates filtrates in different degrees.The results of filtering paper method showed that the distribution rate of J2 with treatments of 12 parasitic fungal isolates filtrates was low on 0.1 cm area. The distribution rates of soybean cyst nematode juvenile(J2) with the treatment of F9-3，V2，F5 and V1 isolates filtrates were 17.3%,17.3%,18.0% and 18.3% in the 0.1 cm area, respectively. It was significantly lower than control in water and root diffusate by 26.0% and 32.3%(P＜0.05). The same result was indicated in direct test. The distribution rates of soybean cyst nematode Juvenile(J2) were between 18.0%-31.3%,in 0.1 cm area with the treatments of soybean root dipped with 12 parasitic fungal fermentation filtrates.It was significantly lower than the control. It was proved that soybean cyst nematode juveniles (J2) were repelled by 12 parasitic fungal fermentation filtrates.

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备注/Memo

基金项目：国家自然科学基金(39071900)。

第一作者简介：鲁建聪(1989-)，女，硕士，主要从事大豆胞囊线虫防治研究。E-mail:cong1048063166@163.com。通讯作者：许艳丽(1958-)，女，研究员，博导，主要从事植物线虫病害、作物病虫害生物生态控制和土壤微生态研究。 E-mail: xyll@iga.ac.cn。

更新日期/Last Update: 2015-09-01