[1]高同国,李术娜,张冬冬,等.大豆根腐病生防细菌优势菌株的筛选、鉴定及生防效果验证[J].大豆科学,2015,34(04):661-665.[doi:10.11861/j.issn.1000-9841.2015.04.0661]
　GAO Tong-guo,LI Shu-na,ZHANG Dong-dong,et al.Screening and Identification of Biocontrol Dominant Bacterium against to Soybean Root Rot[J].Soybean Science,2015,34(04):661-665.[doi:10.11861/j.issn.1000-9841.2015.04.0661]
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大豆根腐病生防细菌优势菌株的筛选、鉴定及生防效果验证

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 661-665 栏目: 出版日期: 2015-08-25
Title:
Screening and Identification of Biocontrol Dominant Bacterium against to Soybean Root Rot
作者:
高同国1; 李术娜1; 张冬冬1; 王灵敏2; 郭晓军1; 朱宝成1
1.河北农业大学 生命科学学院，河北 保定 071001;
2.木美华鼎生物肥料有限公司，河北 唐山 063000
Author(s):
GAO Tong-guo1;  LI Shu-na1;  ZHANG Dong-dong1;  WANG Ling-min2;  GUO Xiao-jun1;  ZHU Bao-cheng1
1.College of Life Science, Agricultural University of Hebei, Baoding 071001, China;?
2.Mumeihuading Biological Fertilizer Co. Ltd, Tangshan 063000, China
关键词:
大豆根腐病;  尖孢镰刀菌;  枯草芽孢杆菌; 生防细菌
Keywords:
Soybean root rot;  Fusarium oxysporum;  Bacillus subtilis;  Biocontrol bacterium
DOI:
10.11861/j.issn.1000-9841.2015.04.0661
文献标志码:
A
摘要:
根腐病是影响大豆产量的主要病害之一，镰刀菌（Fusarium sp）是引起我国大豆根腐病的主要病原菌。以致病尖孢镰刀菌为研究对象，通过平板对峙法从实验室保存的237株芽孢杆菌筛选到一株对该尖孢镰刀菌具有明显抑菌活性的芽孢杆菌8-32。对芽孢杆菌8-32经菌落、菌体形态，16S rRNA基因序列及生理生化性质鉴定，该菌株为枯草芽孢杆菌（Bacillus subtilis）。温室盆栽结果表明：该生防菌对大豆根腐病具有很好的防治效果，其病情指数减少32.08%。
Abstract:
Root rot is one of the most serious diseases which caused reduction of soybean yield, and Fusarium sp is considered as the main pathogen fungi caused this disease in China. In this study, Fusarium oxysporum was taken as indicative fungus, we screened one strain 8-32 against to Fusarium oxysporum from 237 strains saved in our laboratory using the method of dual culture on agar plate.According to colony morphology, 16S rRNA gene sequence, physiological and biochemical characters, we identified this strain as Bacillus subtilis.Results showed that the strain 8-32 reduced the incidence of soybean root rot disease index under controlled glasshouse conditions, which up to 32.08%.This strain, 8-32, has the potential to form production to control the soybean root rot

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备注/Memo

基金项目：保定市科学技术研究与发展指导计划（13ZN023）。

第一作者简介：高同国（1984-），男，博士，讲师，主要从事植物真菌病害的生物防治。 E-mail：gtgrxf@163.com。
通讯作者：朱宝成（1962-），男，博士，教授，主要从事农牧微生物学研究。E-mail：zhu2222@126.com。

更新日期/Last Update: 2015-09-01