[1]张国敏,张亚琴,舒英杰,等.三种大豆种子贮藏蛋白亚基缺失种质的筛选与鉴定[J].大豆科学,2015,34(01):1-8,31.[doi:10.11861/j.issn.1000-9841.2015.01.0001]
　ZHANG Guo-min,ZHANG Ya-qin,SHU Ying-jie,et al.Screening and Identification of Three Types of Soybean Lines Lacking Different Seed Storage Protein Subunits[J].Soybean Science,2015,34(01):1-8,31.[doi:10.11861/j.issn.1000-9841.2015.01.0001]
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三种大豆种子贮藏蛋白亚基缺失种质的筛选与鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年01期 页码: 1-8,31 栏目: 出版日期: 2015-02-25
Title:
Screening and Identification of Three Types of Soybean Lines Lacking Different Seed Storage Protein Subunits
作者:
张国敏1; 张亚琴1; 舒英杰2; 麻浩1
1.南京农业大学 作物遗传与种质创新国家重点实验室/种业科学系，江苏 南京 210095；
2.安徽科技学院 农学院,安徽 凤阳 233100
Author(s):
ZHANG Guo-min1; ZHANG Ya-qin1; SHU Ying-jie2; MA Hao1
1State Key Laboratory of Crop Genetics & Germplasm Enhancement/Department of Seed Industry Science, Nanjing Agricultural University, Nanjing 210095, China；
2Agricultural College,Anhui Science and Technology University,Fengyang 233100,China
关键词:
大豆种质创新; 贮藏蛋白; 亚基缺失; 创制; 评价
Keywords:
Soybean (Glycine maxL.Merr.) ;  Storage protein;  Subunit deficiency;  Germplasm enhancement;  Evalution
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0001
文献标志码:
A
摘要:
蛋白质组分改良是国内外大豆蛋白质品质育种的研究热点之一。大豆种子贮藏蛋白主要包括11S球蛋白和7S-β伴大豆球蛋白，它们也是大豆蛋白营养价值和功能特性的决定组分。利用我国自然突变产生的亚基缺失体材料通过聚合育种，并经聚丙烯酰胺凝胶电泳法（SDS-PAGE）分析鉴定表明：已获得A3+α’亚基缺失、只含有β亚基、只含有α+β亚基、只含有α’+β亚基、只含有7S亚基和A5A4B3亚基缺失、A2A1aA1b含量极低、B1aB1bB2含量极低等系列单缺、双缺、多缺的贮藏蛋白亚基组成类型新种质，其中A3+α’亚基缺失、只含有α+β亚基、只含有7S亚基3种种质均能正常生长、结实，并稳定遗传；只含7S亚基类型大豆种子贮藏蛋白的7S组分总量含量最高；A3+α’亚基缺失类型大豆种子贮藏蛋白11S组分总含量最高；在11S+7S组分总量上由高到低依次是A3+α’亚基缺失类型>只含7S亚基类型>只含α+β亚基类型；11S/7S比值的变异范围在0.14~1.27。三种类型种质完熟期基本在105~111 d；百粒重基本一致；A3+α’亚基缺失类型大豆单株产量最高平均为55.12 g；只含α+β亚基类型蛋脂总量最低为57.6%，蛋白质含量由高到低依次为只含7S亚基类型、A3+α’亚基缺失类型、只含α+β亚基类型，脂肪含量由高到低依次为：只含α+β亚基类型、只含7S亚基类型、A3+α’亚基缺失类型。
Abstract:
Improvement of protein components is one of the hotspots in the protein quality breeding of soybean. Soybean seed storage proteins mainly consist of 11S globulin and 7Sβ-conglycinin. They determine the nutritional value and functional properties of soy proteins. In the present study, natural mutants of subunit deficiency were used through pyramiding breeding to produce new deficiency types. After screening with polyacrylamide gel electrophoresis (SDS-PAGE), lines lacking A3+α′subunits, lines only containing βsubunit, lines only containing α+βsubunits, lines only containing α′+βsubunits, lines only containing 7S subunits, lines lacking A5A4B3 subunits, lines with the lower level of A2A1aA1b?subunit and lines with the lower level of B1aB1bB2 subunit, etc, were obtained. The lines lacking A3+α′ subunits, containing only α′+β subunits, and containing 7S subunits could normally grow and develop as well as were stably inherited. The lines containing only 7S subunits had the highest amount of total 7S while the lines lacking A3+α’ subunits possessed the highest level of 11S. The variation range for the 11S/7S ratio in the three new types was between 0.14 and 1.27, while their growth period was between 105 and 111 d. All the three new types had almost the same hundred-grain weight. The lines lack of A3+α′ subunits had the highest yield per plant of 55.12 g. The lines containing only α+β subunits had the lowest amount of protein and oil (57.6%). The order of protein contents of the lines were as follows: lines containing 7S subunits>lines lacking A3+α’ subunits>lines containing only α+βsubunits. The order of fat content was as follows: lines containing only α+βsubunits>lines containing only 7S subunits>lines lacking A3+α’ subunits.

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备注/Memo

基金项目：上海市科委重点攻关项目(12391900900);国家自然科学基金(30971840，31171572，31371711);高等学校博士学科点专项科研基金(20100097110030, 20120097110025);江苏省高校优势学科建设工程资助项目。

第一作者简介：张国敏(1989-)，女，硕士，主要从事作物种质资源遗传改良研究。E-mail：vivyear@sian.cn。
通讯作者：麻浩(1965-)，男，教授，主要从事种子科学与大豆遗传育种研究。E-mail: Lq-ncsi@njau.edu.cn。

更新日期/Last Update: 2015-04-09