[1]王力,刘辉,张梦媛,等.三羟异黄酮对全氟辛酸暴露的小鼠肝脏抗氧化能力的影响[J].大豆科学,2014,33(06):937-939,944.[doi:10.11861/j.issn.1000-9841.2014.06.0937]
　WANG Li,LIU Hui,ZHANG Meng-yuan,et al.Effects of Genistein on Relevant Enzyme Activity in Liver of Mice Exposed to Perfluorooctanoic Acid[J].Soybean Science,2014,33(06):937-939,944.[doi:10.11861/j.issn.1000-9841.2014.06.0937]
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三羟异黄酮对全氟辛酸暴露的小鼠肝脏抗氧化能力的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年06期 页码: 937-939,944 栏目: 出版日期: 2014-12-25
Title:
Effects of Genistein on Relevant Enzyme Activity in Liver of Mice Exposed to Perfluorooctanoic Acid
文章编号:
1000-9841.2014.06.0937
作者:
王力1; 刘辉2; 张梦媛1; 吕卉1; 申玲1
1.蚌埠医学院 预防医学系，安徽 蚌埠 233030；
2.蚌埠医学院 生物科学系,安徽 蚌埠 233030
Author(s):
WANG Li1; LIU Hui2; ZHANG Meng-yuan1; LYU Hui1; SHEN Ling1
1.Department of Preventive Medicine,Bengbu Medical College,Bengbu 233030,China;
2.Department of Bioscience,Bengbu Medical College,Bengbu 233030,China
关键词:
全氟辛酸; 三羟异黄酮; 肝组织; 氧化损伤
Keywords:
Perfluorooctanoic acid; Genistein; Liver; Oxidative damage
分类号:
Q657.32
DOI:
10.11861/j.issn.1000-9841.2014.06.0937
文献标志码:
A
摘要:
将80只SPF级ICR小鼠按单纯随机抽样方法分为正常对照组（等体积生理盐水)、PFOA低剂量组(1 mg.kg-1.d-1)、PFOA中剂量组(5 mg.kg-1.d-1)、PFOA高剂量组(25 mg.kg-1.d-1)、GEN干预对照组(20 mg.kg-1.d-1)、GEN干预PFOA低剂量组(GEN：20 mg.kg-1.d-1、PFOA：1 mg.kg-1.d-1)、GEN干预PFOA中剂量组(GEN：20 mg.kg-1.d-1、PFOA：5 mg.kg-1.d-1)、GEN干预PFOA高剂量(GEN：20 mg.kg-1.d-1、PFOA：25 mg.kg-1.d-1)组，每组10只，采用灌胃方法，通过计算肝脏比体重系数，检测肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)、乳酸脱氢酶(LDH)、丙二醛(MDA)及一氧化氮(NO)指标的水平，探讨了三羟异黄酮（GEN)对不同剂量的全氟辛酸（PFOA)致小鼠肝脏氧化损伤的保护作用。结果表明：PFOA导致肝脏肿大，增加了小鼠肝脏系数，并随着剂量增加而加重。高剂量的PFOA能明显增高肝脏组织中MDA及NO含量（P<0.05)，低、中、高剂量的PFOA均能提高LDH活性和降低SOD、GSH-Px的活性（P<0.05)，而GEN在干预PFOA各剂量组中能显著提高GSH-Px的活性和降低LDH活性（P<0.05)，在干预PFOA高剂量组中能提高SOD的活性（P<0.05)。GEN对PFOA致肝脏组织的脂质过氧化损伤有一定的拮抗作用。
Abstract:
80 mice were distributed randomly into normal control group（the same dose of normal saline)，PFOA low dose group（1 mg.kg-1.d-1)，PFOA medium dose group（5 mg.kg-1.d-1)，PFOA high dose group（25 mg.kg-1.d-1)，GEN intervention group(20 mg.kg-1.d-1)，GEN intervention low dose group(GEN：20 mg.kg-1.d-1and PFOA：1 mg.kg-1.d-1)，GEN intervention medium dose group(GEN：20 mg.kg-1.d-1and PFOA：5 mg.kg-1.d-1)，GEN intervention high dose group(GEN：20 mg.kg-1.d-1and PFOA：25 mg.kg-1.d-1).The mice were weighed every day and their behavior were observed.After 14 days，the mice were killed and liver indexes were calculated.Meanwhile,the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),lactate dehydrogenase(LDH)and the content of malondialdehyde(MDA)and nitric oxide(NO)were also assayed.The results showed that the content of MDA and NO were increased dramatically in PFOA high dose group（P<0.05)，the activity of LDH in hepatic tissue of PFOA groups were increased dramatically while the activity of SOD and GSH-Px was decreased obviously compared with the control group in 3 dose group of PFOA（P<0.05)，GEN could increase the activity of GSHPx and decrease the activity of LDH in 3 GEN intervention groups（P<0.05)，while the activity of SOD was increased in GEN intervention high dose group（P<0.05).Supplementation of GEN during PFOA exposure may have certain protective effect on inhibition caused by PFOA exposure.

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备注/Memo

基金项目：蚌埠医学院省级特色专业“预防医学”专项基金(皖教高〔2011〕5号)；安徽省高校优秀青年人才基金资助项目（2012SQRL091）。

第一作者简介：王力（1982-），女，硕士，讲师,主要从事营养毒理学研究。 E-mail: lilywang990@aliyun.com。

更新日期/Last Update: 2014-12-29