RNAi在植物寄生线虫抗性研究中靶基因的选择策略

参考文献/References:

[1]Bernstein E，Caudy A A，Hammond S M，et al.Role for a bidentate ribonuclease in the initiation step of RNA interference[J].Nature?2001，409:363-366.

[2] Guo S，Kemphues K.Par-1，a gene required for establishing polarity in?C.elegansembroys,encodes a putative Ser/Thr kinse that is asymmetrically distributed[J].Cell，1995，81:611-620.

[3] Fire A，Xu S，Montgomery M K，et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature，1998，391:806-811.

[4] Kawasaki H,Taira K.Short hairpin type of dsRNA that are controlled by tRNA(Val)promoter significantly induce RNAi mediated gene silencing in the cytoplasm of human cells[J].Nucleic Acids Research，2003，31:7002-7007.

[5] 石智，符立梧.RNAi及其在肿瘤研究中的应用[J].生物化学与生物物理展，2004，31:492-499.（Shi Z,Fu L W.RNAi and its application in tumor study[J].Progress in Biochemistry and Biophysics,2004,31:492-499.）

[6] Caplen N J，Fleenor J，Fire A，et al.dsRNA-mediated gene silencing in cultured Drosophilacells[J].Gene，2000，252:95-105.

[7] Palauqui J C，Elmayan T，Pollien J M，et al.Systemic acquired silencing:transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions[J].EMBO Journal，1997，16:4738-4745.

[8] Zamore P D，Tuschi T，Sharp P A，et al.RNAi:dsRNA directs the ATP dependent cleavage of mRNA at 21 to 23 nucleotide intervals[J].Cell，1999，101:25-33.

[9] Jones L，Ratcliff F，Baulcombe D C.RNA-directed transcriptional gene silencing in plants can be inherited independently of the RNA trigger and requires Met1for maintenance[J].Current Biology，2001，11:747-757.

[10]Winston W M，Molodowtich C，Hunter C P.Systemic RNAi in C.elegansrequires the putative transmembrane protein SID-1[J].Science，2002，295:2459-2459.

[11]Timmons L，Fire A.Specific interference by ingested dsRNA[J].Nature，1998，395:854.

[12]Timmons L，Court D L.Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans[J].Gene，2001，263:103-112.

[13]Mello C C，Conte D J.Revealing the world of RNA interference[J].Nature，2004，431:338-342.

[14]Sindhu A S，Maier T R，Mitchum M G，et al.Effective and specific in planta RNAi in cyst nematodes:expression interference of four parasitism genes reduces parasitic success[J].Journal of Experimental Botany，2009，60:315-324.

[15]Rosso M N，Jones J T，Abad P.RNAi and functional genomics in plant parasitic nematodes[J].Annual Review of Phytopathology，2009，47:207-232.

[16]Pramod P K，Heinz R，Yeckel G，et al.A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine ma[J].BMC Research Notes，2013，6:255.

[17]Dinh P T，Knoblaucn M，Elling A A.Non-destructive imaging of plant-parasitic nematode development and host response to nematode pathogenesis[J].Phytopathology，2014，104：497-506.

[18]Li J R，Todd T，Lee J，et al.Biotechnological application of functional genomics towards plant-parasitic nematode control[J].Plant Biotechnology Journal，2011，9:936-944.

[19]Xue B，Hamamouch N，Li C，et al.The 8D05 parasitism gene of Meloidogyne incognita is required for successful infection of host roots[J].Phytopathology，2013，103:175-181.

[20]Bakhetia M，Charlton W，Atkinson H J，et al.RNA interference of dual oxidase in the plant nematode Meloidogyne incognita[J].Molecular Plant-Microbe Interactactions，2005，18:1099-1106.

[21]Chen Q，Reham S，Smant G，et al.Functional analysis of pathogenicity proteins of the potato cyst nematode Globodera rostochiensisusing RNAi[J].Molecular Plant-Microbe Interactactions，2005，18:621-625.

[22]Bakhetia M，Urwin P，Atkinson H J.qPCR analysis and RNAi define pharyngeal gland cell expressed genes of?Heterodera glycinesrequired for initial interactions with the host[J].Molecular Plant-Microbe Interactions，2007，20:306-312.

[23]Hu L，Cui R，Sun L，et al.Molecular and biochemical characterization of the β-1,4-endoglucanase gene Mj-eng-3 in the root-knot nematode Meloidogyne javanica[J].Experimental Parasitology，2013，135:15-23.

[24]Shingles J，Lilley C，Atkinson H J，et al.Meloidogyne incognita：molecular and biochemical characterization of a cathepsin L cysteine proteinase and the effect on parasitism following RNAi[J].Experimental Parasitology，2007，115:114-120.

[25]Huang G，Allen R，Davis E L，et al.Enginering broad root-knot resistance intransgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene[J].Proceedings of the National Academy of Sciences of the USA，2006，103:14302-14306.

[26]Ibrahim H M，Alkharouf N W，Meyer S L，et al.Post-transcriptional gene silencing of root-knot nematode in transformed soybean roots[J].Experimental Parasitology，2011，127:90-99.

[27]Dubreuil G，Magliano M，Deleury E，et al.Transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism[J].New Phytologist，2007，176:426-436.

[28]Urwin P E，Lilley C J，Atkinson H J.Ingestion of double stranded RNA by pre-parasitic juvenile cyst nematodes leads to RNA interference[J].Molecular Plant-Microbe Interactions，2002，5:747-752.

[29]Abad P，Gouzy J，Aury J，et al.Plant parasitism in metazoans:insights from the Meloidogyne incognitanematode genome[J].Nature Biotechnology，2008，26:909-915.

[30]Alkharouf N W，Klink V P，Matthews B F.Identification of Heterodera glycines(soybean cyst nematode[SCN])cDNA sequences with high identity to those of?Caenorhabditis eleganshaving lethal mutant or RNAi phenotypes[J].Experimental Parasitology，2007，115:247-258.

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