[1]李永光,张宇航,罗秋兰,等.大豆GmPLP1启动子的克隆及功能分析[J].大豆科学,2014,33(03):309-314.[doi:10.11861/j.issn.1000-9841.2014.03.0311]
　LI Yong-guang,ZHANG Yu-hang,LUO Qiu-lan,et al.Cloning and Functional Analysis of a Promoter of GmPLP1 Gene in Soybean[J].Soybean Science,2014,33(03):309-314.[doi:10.11861/j.issn.1000-9841.2014.03.0311]
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大豆GmPLP1启动子的克隆及功能分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年03期 页码: 309-314 栏目: 出版日期: 2014-06-25
Title:
Cloning and Functional Analysis of a Promoter of GmPLP1 Gene in Soybean
作者:
李永光; 张宇航; 罗秋兰; 谷翰卿; 李文滨
东北农业大学大豆生物学教育部重点实验室，东北农业大学农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030
Author(s):
LI Yong-guang; ZHANG Yu-hang; LUO Qiu-lan; GU Han-qing; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China
关键词:
GmPLP1启动子; GUS基因; 组织化学染色; 顺式调控元件
Keywords:
GmPLP1 promoter; GUSgene; Histochemical staining; Cisregulatory elements
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0311
文献标志码:
A
摘要:
本研究利用染色体步移法从大豆栽培品种东农L13基因组中分离得到了GmPLP1基因1.5 kb的启动子片段，利用PLACE和PlantCARE在线启动子预测工具发现了107个调控元件。将该片段定向替换载体pBI121的CaMV35S组成型启动子，构建表达载体pBI121-pGmPLP1，驱动下游报告基因GUS基因表达，并通过组织化学染色和GUS定量分析对预测结果进行验证，结果显示GmPLP1受黑暗、蓝光、低温、GA3和ABA诱导表达。
Abstract:
To reveal the molecular mechanism underlying the expression of soybean PLP1(GmPLP1)gene that contains PAS/LOV domain,its 5′ regulatory region was analyzed.The1.5 kb fragment upstream of the GmPLP1 gene was isolated from soybean cultivar ‘Dongnong L13’by genome walking.There were 107 cisacting elements in the promoter predicted by online software Plant CARE and PLACE.the promoter fragment deletions were inserted into the 5′ region of the gus.A reporter gene in vector pBI121(replacing the 35S promoter).In order to determine the promoter characteristic of GmPLP1.The constructs were transferred into Nicotiana tabacum and Glycine max.The GUS activity was upregulated under dark,blue light,gibberellin A3 and abscisic acid driven by GmPLP1promoter.

参考文献/References:


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备注/Memo

收稿日期：2013-09-13

基金项目：抗逆转基因大豆新品种培育 (2014ZX08004-002)；国家自然科学基金(31201228)。
第一作者简介：李永光(1984-)，男，博士，助理研究员，主要从事大豆生物技术研究。E-mail:yongguangli@neau.edu.cn。
通讯作者：李文滨(1958-)，男，博士，教授，主要从事大豆遗传育种研究。E-mail:wenbinli@neau.edu.cn。

更新日期/Last Update: 2014-08-01