[1]刘 莹,刘 政,赵 杰,等.超声波辅助酶法制备大豆抗氧化肽[J].大豆科学,2014,33(02):281-283.[doi:10.11861/j.issn.1000-9841.2014.02.0281]
　LIU Ying,LIU Zheng,ZHAO Jie,et al.Preparation of Soybean Antioxidant Peptide by Ultrasonic-assisted Enzymatic Method[J].Soybean Science,2014,33(02):281-283.[doi:10.11861/j.issn.1000-9841.2014.02.0281]
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超声波辅助酶法制备大豆抗氧化肽

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年02期 页码: 281-283 栏目: 出版日期: 2014-04-24
Title:
Preparation of Soybean Antioxidant Peptide by Ultrasonic-assisted Enzymatic Method
文章编号:
1000-9841（2014）02-0281-03
作者:
刘 莹1; 刘 政1; 赵 杰2; 许 琳2
1.辽宁工程技术大学 理学院，辽宁 阜新 123000;?
2.辽宁工程技术大学 材料学院，辽宁 阜新 123000
Author(s):
LIU Ying1; LIU Zheng1; ZHAO Jie2; XU Lin2
1.College of Science,Liaoning Technical University,Fuxin 123000,China;
2.College of Material,Liaoning Technical University,Fuxin 123000,China
关键词:
大豆分离蛋白; 大豆抗氧化肽; 超声波辅助; 水解度; 还原力
Keywords:
Soybean isolated protein; Soybean antioxidant peptide; Ultrasonic-assisted; Hydrolysis level; Reducing power
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.02.0281
文献标志码:
A
摘要:
以大豆分离蛋白为底物，利用碱性蛋白酶和胰蛋白酶酶解大豆蛋白制取大豆抗氧化肽。以还原力为指标，探讨了酶解制取大豆抗氧化肽的最佳工艺，并研究了超声波对酶解制取大豆抗氧化肽的影响。结果表明：超声波处理能提高大豆蛋白的还原力，并且在超声波和双酶同时处理条件下，最佳条件为底物浓度5%，pH9.0，温度50℃，时间5 h。在该条件下，酶解液的吸光度为0.124。
Abstract:
In this experiment,using isolated soybean protein as substrate,the alkaline protease and trypsin were used to hydrolyze soybean protein to prepare soybean antioxidant peptide.Taking reducing power as an indicator,the optimum of the preparation of soybean antioxidant by the enzymatic method was discussed,at the same time the impact of ultrasound on the preparation of the soybean antioxidant peptide by the enzymatic method was studied.The results showed ultrasonic could increase the reducing power of soybean protein,and under the condition of double enzymes and ultrasonic simultaneously,optimal conditions was reaction at pH9.0,50℃ for 5 h with substrate concentration of 5%.In this condition,the absorbance was 0.124.

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备注/Memo


第一作者简介：刘莹（1970-）,男，硕士，副教授，主要从事食品化学研究。E-mail:liuyingfx02@126.com。

更新日期/Last Update: 2014-08-05