[1]李淑芬,胡 敏.碱溶酸沉法提取大豆蛋白条件的优化[J].大豆科学,2014,33(02):274-276,280.[doi:10.11861/j.issn.1000-9841.2014.02.0274]
　LI Shu-fen,HU Min.Optimizition of Soybean Protein Extraction by Alkali Soluble Acid Sinking Method[J].Soybean Science,2014,33(02):274-276,280.[doi:10.11861/j.issn.1000-9841.2014.02.0274]
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碱溶酸沉法提取大豆蛋白条件的优化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年02期 页码: 274-276,280 栏目: 出版日期: 2014-04-24
Title:
Optimizition of Soybean Protein Extraction by Alkali Soluble Acid Sinking Method
文章编号:
1000-9841（2014）02-0274-03
作者:
李淑芬1; 胡 敏2
1.东北师范大学 生命科学学院，吉林 长春 130024；
?2.大庆师范学院 生命科学学院，黑龙江 大庆 163712
Author(s):
LI Shu-fen1; HU Min2
1.College of Life Science,Northeast Normal University,Changchun 130024,China;
2.College of Life Science,Daqing Normal University,Daqing 163712,China
关键词:
大豆蛋白; 碱溶酸沉法; 优化
Keywords:
Soybean protein; Alkali solution and acid; Optimization
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.02.0274
文献标志码:
A
摘要:
以大豆为材料，采用碱溶酸沉法提取大豆蛋白。分别通过单因素试验和正交试验对固料比、碱溶pH、酸沉pH、碱溶温度和碱溶时间进行优化。最终确定最佳工艺为固料比1∶10、酸沉pH4.5、碱溶pH9.5、碱溶时间50 min、碱溶温度45℃。在此条件下，大豆蛋白的提取得率达25.88%。
Abstract:
Soybean protein was extracted from soybean seed powder using alkali soluble and acid sinking method.The extracting conditions including the ratio of material to water,alkali-soluble pH,acid precipitation pH,temperature and alkali-soluble time were optimized by single-factor experiment and orthogonal test.Results showed the optimum condition were as follows: the ratio of solid material to water was 1∶10,acid precipitation pH was 4.5,alkali-soluble pH was 9.5,alkali-soluble time was 50 min and the alkali solution temperature was 45℃.In this case,the extraction rate of soybean protein reached 25.88%.

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备注/Memo


第一作者简介：李淑芬（1988-），女，在读硕士，主要从事学科教学(生物)中学教学。E-mail：shufendajie@163.com。

更新日期/Last Update: 2014-08-05