[1]赵路苹,陈业明,张彩猛,等.提取条件对大豆油体表面蛋白质去除效果的研究[J].大豆科学,2013,32(06):835-839.[doi:10.11861/j.issn.1000-9841.2013.06.0835]
　ZHAO Lu-ping,CHEN Ye-ming,ZHANG Cai-meng,et al.Removing Effects of Extract Conditions on Surface Proteins from Soybean Oil Body[J].Soybean Science,2013,32(06):835-839.[doi:10.11861/j.issn.1000-9841.2013.06.0835]
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提取条件对大豆油体表面蛋白质去除效果的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年06期 页码: 835-839 栏目: 出版日期: 2013-12-25
Title:
Removing Effects of Extract Conditions on Surface Proteins from Soybean Oil Body
作者:
?赵路苹; 陈业明; 张彩猛; 华欲飞
?（江南大学 食品学院，江苏 无锡 214122）
Author(s):
?ZHAO Lu-ping; CHEN Ye-ming; ZHANG Cai-meng; HUA Yu-fei
?(School of Food Science and Technology，Jiangnan University，Wuxi 214122，China)
关键词:
大豆油体; 油体蛋白; 大豆蛋白质; 提取条件; SDS-PAGE
Keywords:
Soybean oil body; Oleosin; Soybean proteins; Extract conditions; SDS-PAGE
DOI:
10.11861/j.issn.1000-9841.2013.06.0835
文献标志码:
A
摘要:
??在确定生豆浆一次超速离心提取大豆油体的适宜蔗糖浓度为10%的基础上，研究了氯化钠、尿素、EDTA-2Na、SDS和温度等提取条件对大豆油体表面蛋白质的影响，并通过SDS聚丙烯酰胺凝胶电泳表征油体表面蛋白质的变化。结果表明：大于6 mol·L-1尿素和80～100℃加热处理15 min都有利于去除油体表面结合的大豆蛋白质，得到高纯度的油体；氯化钠和EDTA-2Na对去除表面结合的大豆蛋白质没有作用；SDS可以取代油体蛋白，破坏油体天然结构，不利于油体的提取。
Abstract:
?After confirmed 10% sucrose was beneficial for extracting oil body from raw soybean milk at once ultra centrifugation,the effects of NaCl，urea，EDTA-2Na，SDS and different temperatures on surface proteins of soybean oil body were studied.The variation of oil body surface proteins was characterized by SDSpolyacrylamide gel electrophoresis(SDSPAGE).The results suggested that more than 6 mol·L-1 urea and thermal treatment(80-100℃，15 min)were beneficial for removing soybean proteins which combined with oil body.However，NaCl and EDTA-2Na had no effect on removal of soybean proteins.While SDS was bad for oil body extraction because it could replace oleosins and breake natural structure of oil body.

备注/Memo

?2012年教育部基本科研—青年基金（2050205）。

更新日期/Last Update: 2014-04-04