[1]徐玲莉,祖永平,郭顺堂,等.氟磺胺草醚与植物蛋白相互作用的研究[J].大豆科学,2013,32(06):763-767.[doi:10.11861/j.issn.1000-9841.2013.06.0763]
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氟磺胺草醚与植物蛋白相互作用的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年06期 页码: 763-767 栏目: 出版日期: 2013-12-25
Title:
Interaction between Fomesafen and Vegetable Protein
作者:
?徐玲莉1; 祖永平1; 郭顺堂2; 陶 波1
?（1.东北农业大学 农学院，黑龙江 哈尔滨 150030； 2.中国农业大学 食品科学与营养工程学院，北京 100083）
Author(s):
?(1.College of Agriculture，Northeast Agricultural University，Harbin 150030，China;2.College of Food Science ＆ Nutritional Engineering，China Agricultural University，Beijing 100083，China)
关键词:
氟磺胺草醚; 牛血清白蛋白; 植物蛋白; 荧光; 热力学参数
Keywords:
Fomesafen; Bovine serum albumin(BSA); Vegetable protein; Fluorescence; Thermodynamic parameter
DOI:
10.11861/j.issn.1000-9841.2013.06.0763
文献标志码:
A
摘要:
?在模拟动物体生理条件下，利用荧光光谱方法，以牛血清蛋白(BSA)为对照，对除草剂氟磺胺草醚与植物蛋白（11S蛋白、酶解后11S蛋白）的互作效应进行研究。分析蛋白发生变性反应后，氟磺胺草醚与牛血清白蛋白、植物蛋白结合常数和结合位点数的变化情况。结果表明：氟磺胺草醚对蛋白有较强的荧光猝灭作用，氟磺胺草醚与牛血清、11S蛋白、酶解后11S蛋白发生结合反应生成了新的复合物，属于静态荧光猝灭。根据不同温度下的热力学参数可以推断：氟磺胺草醚与牛血清白蛋白之间的作用力主要是疏水作用力；与11S蛋白及酶解后的11S蛋白之间的作用力均为氢键和范德华力。将3种蛋白分别进行热处理、超声振荡、金属盐离子和酸性处理后，氟磺胺草醚与蛋白的结合反应发生了不同程度的变化。结果为阐明氟磺胺草醚的毒理学提供一定的理论依据。
Abstract:
?Taking the bovine serum albumin(BSA) as control,the interaction between fomesafen and vegetable protein was studied by fluorescence spectrum under the imitated physiological condition of animal body.Changes of the binding constant and the number of binding site of the fomesafen with BSA and vegetable protein after the protein denaturalization reaction were analyzed. Fomesafen had a strong ability to quench the fluorescence launching from BSA and vegetable protein.The BSA and vegetable protein had reacted with fomesafen and they formed a certain new compound，and its belonged to static fluorescence queching reaction.The thermodynamic parameters obtained at different temperature suggested that the binding power between the BSA and fomesafen was mainly the hydrophobic interaction，between the vegetable protein and fomsafen was mainly hydrogen bonding/van der Waals.When the three proteins were subjected to heat，ultrasonic，metal salts/sonic and acidity treatment，their binding reaction with fomesafen varied to some extent.This essay will supply a theory base for the poisoning mechanism of fomesafen.

相似文献/References:

[1]王恒亮,葛玉红,苏旺苍,等.不同缓解处理对氟磺胺草醚大豆药害的缓解效果研究[J].大豆科学,2013,32(05):676.[doi:10.11861/j.issn.1000-9841.2013.05.0676]
　WANG Heng-liang,GE Yu-hong,SU Wang-cang,et al.Effects of Different Mitigation Treatments on the Phytotoxicity of Fomesafen in Soybean[J].Soybean Science,2013,32(06):676.[doi:10.11861/j.issn.1000-9841.2013.05.0676]

备注/Memo

?国家自然科学基金（30971834）。

更新日期/Last Update: 2014-02-28