[1]刘少静,王多宁,刁颖博,等.大豆异黄酮纯化工艺研究[J].大豆科学,2013,32(04):535-538.[doi:10.11861/j.issn.1000-9841.2013.04.0535]
　LIU Shao-jing,WANG Duo-ning,DIAO Ying-bo,et al.Optimization on the Purification Technologies of Soybean Isoflavone[J].Soybean Science,2013,32(04):535-538.[doi:10.11861/j.issn.1000-9841.2013.04.0535]
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大豆异黄酮纯化工艺研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年04期 页码: 535-538 栏目: 出版日期: 2013-08-25
Title:
Optimization on the Purification Technologies of Soybean Isoflavone
作者:
?刘少静1; 王多宁2; 刁颖博1; 肖秋茹1; 张玉琪1; 杨黎彬1
?1.西安医学院 药学院，陕西 西安 710021；2.西安医学院 科研中心，陕西 西安 710021
Author(s):
?LIU Shao-jing1; WANG Duo-ning2; DIAO Ying-bo1; XIAO Qiu-ru1; ZHANG Yu-qi1; YANG Li-bin1
?1.College of Pharmacy,Xi’an Medical University,Xi’an 710021,China;2.Scientific Research Center,Xi’an Medical University,Xi’an 710021,China
关键词:
豆粕; 大豆异黄酮; 纯化; 醇沉法; 萃取
Keywords:
Soybean meal; Soybean isoflavone; Purification; Ethanol precipitation; Extraction
分类号:
TS202.3
DOI:
10.11861/j.issn.1000-9841.2013.04.0535
文献标志码:
A
摘要:
?以大豆异黄酮纯度为考察指标，将醇沉结合乙酸乙酯萃取法与其他纯化方法进行对比，并进一步对醇沉法中工艺参数通过单因素试验及正交试验进行优化。结果表明：醇沉最佳工艺为浓缩液质量分数25%，乙醇浓度80%，搅拌时间13 min，静置时间5 h；经等体积乙酸乙酯萃取3次，异黄酮基本萃取完全。醇沉结合乙酸乙酯萃取法可得到含量高达16.83%的大豆异黄酮产品，比单纯醇沉法提高约4倍。
Abstract:
?With the purity of soybean isoflavone as investigation index,the isoflavone purification method of alcohol precipitation combined with ethyl acetate extraction was compared with other methods.And then the alcohol precipitation parameters for the purification of isoflavones were optimized by single-factor and orthogonal experiment.The optimized conditions of ethanol precipitation were,mixing 25% mass fraction of isoflavone extract with 80% ethanol for 13 min,and then standing for 5 h;After 3 times extraction with ethyl acetate,isoflavones could be extracted completely.Under the new technology of alcohol precipitation and ethyl acetate extraction,the purification of isoflavones in final product was 16.83%,increased nearly 4 times than separate ethanol precipitation.

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备注/Memo

?基金项目：2011年榆林科技局产学研项目。

更新日期/Last Update: 2014-04-04