[1]陈丽丽,刘晓冬,赵洪锟,等.福建湄洲岛烟豆(G.tabacina)遗传多样性分析[J].大豆科学,2013,32(03):286-290.[doi:10.11861/j.issn.1000-9841.2013.03.0286]
　CHEN Li-li,LIU Xiao-dong,ZHAO Hong-kun,et al.Genetic Diversity of G.tabacina from Meizhou Island of Fujian Province[J].Soybean Science,2013,32(03):286-290.[doi:10.11861/j.issn.1000-9841.2013.03.0286]
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福建湄洲岛烟豆(G.tabacina)遗传多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年03期 页码: 286-290 栏目: 出版日期: 2013-06-25
Title:
Genetic Diversity of G.tabacina from Meizhou Island of Fujian Province
作者:
?陈丽丽1; 刘晓冬2; 赵洪锟2; 袁翠平2; 王英男2; 许明子1; 王玉民2
1.延边大学 农学院，吉林 延吉 133000；2.吉林省农业科学院 农业生物技术研究所，吉林 长春 130033
Author(s):
?CHEN Li-li 1; LIU Xiao-dong 2; ZHAO Hong-kun 2; YUAN Cui-ping 2; WANG Ying-nan 2; XU Ming-zi 1; WANG Yu-min 2
?1.College of Agronomy,Yanbian University,Yanji 133000，China;2.Biotechnology Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033，China
关键词:
ISSR标记; 烟豆; 遗传多样性
Keywords:
ISSR marker; G.tabacina; Genetic diversity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.03.0286
文献标志码:
A
摘要:
?采用20个ISSR标记对来自福建湄洲岛的40份烟豆（G.tabacina）材料进行了遗传多样性分析以促进烟豆资源的保护开发和利用。结果表明：20个ISSR引物对40份烟豆基因组DNA均可进行有效扩增，共扩增出240条谱带，其中多态性谱带229条，多态性比率为95.4%；20个ISSR引物的多态性信息含量为0.03～0.37，平均为0.27；40份烟豆材料之间的Dice相似系数为0.335 6～0.863 9，平均为0.675 3，说明湄洲岛烟豆材料遗传变异较丰富；聚类分析将40份烟豆材料划分为4个类群，每个类群都包括来源于不同采集地点的材料，说明不同采集地点之间的材料存在基因交流。
Abstract:
?In this study,the genetic diversity of 40 accessions of G.tabacina,collected from Meizhou Island of Fujian province were analyzed using 20 ISSR markers to promote the protection,exploration and utilization of G.tabacina.A total of 240 bands were amplified,of which 229 bands,accounting for 95.4%,were polymorphic.The average polymorphism information content(PIC)of 20 ISSR primers was 0.27,ranging from 0.03 to 0.37.The Dice similarity coefficient of the tested accessions ranged from 0.335 6 to 0.863 9,and averaged 0.675 3.The tested G.tabacina were clustered into four groups by using the unweighted pairgroup method with arithmetic averaging(UPGMA),and each group contained one or more accessions from different collection sites.Results suggest there is rich of genetic diversity among the accessions of G.tabacina,and existing gene exchange among G.tabacina ?accessions from different collection sites.

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更新日期/Last Update: 2014-03-04