[1]谷月娇,赵 琳,张彦威,等. 大豆GmGAMYB1启动子克隆与分析[J].大豆科学,2013,32(02):0.[doi:10.3969/j.issn.1000-9841.2013.02.005]
　GU Yue-jiao,ZHAO Lin,ZHANG Yan-wei,et al. Isolation and Analysis of Promoter of GmGAMYB1 from Soybean[J].Soybean Science,2013,32(02):0.[doi:10.3969/j.issn.1000-9841.2013.02.005]
点击复制

 大豆GmGAMYB1启动子克隆与分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年02期 页码: 0 栏目: 出版日期: 2013-04-25
Title:
 Isolation and Analysis of Promoter of GmGAMYB1 from Soybean
作者:
 谷月娇; 赵 琳; 张彦威; 卢清瑶; 宋仙萍; 李文滨
 （大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室/东北农业大学大豆研究所，黑龙江 哈尔滨 150030）
Author(s):
 GU Yue-jiao; ZHAO Lin; ZHANG Yan-wei; LU Qing-yao; SONG Xian-ping; LI Wen-bin
关键词:
 ; GmGAMYB1启动子; GUS基因; 组织化学染色
Keywords:
 ; GmGAMYB1 ;  promoter; GUS gene; Histochemical staining
DOI:
10.3969/j.issn.1000-9841.2013.02.005
文献标志码:
A
摘要:
 利用PCR技术从大豆品种东农42基因组中分离得到了GmGAMYB1基因启动子片段pGmGAMYB1，定向替换载体pBI121的CAMV35S组成型启动子，构建表达载体pBI121pGmGAMYB1，驱动下游报告基因GUS基因表达，并利用PLACE和PlantCARE在线启动子预测工具进行分析。结果表明：序列中含有多种典型的光及各种激素和胁迫诱导特异性表达元件，如光应答元件如3-AF1 binding site、BOX-4、T-Box和TCT-motif；赤霉素应答元件GARE；脱落酸应答元件ABRE；热激元件CCAATBox；节律钟Ciradian等。
Abstract:
 Soybean(Glycine max)is a kind of typical plant in the developmental biology and molecular biology research,the completion of soybean genome has provided a lot of convenience for research,but there is still so little achievement on the soybean highly effective inducible promoters.Gibberellin(GA) biosynthesis and signaling pathways have been discussed in detail in recent years.In addition,several reviews discuss general mechanisms of morphogenesis.GAMYB1 gene is an important promotive factor in the signal transduction pathways of GA.In this study,the promoter of GmGAMYB1 was cloned from the soybean Dongnong 42 by PCR.It was directionally replaced CAMV 35S constitutive promoter of the vector pBI121.The expression vector of pBI121pGmGAMYB1 was constructed and the expression of downstream reporter GUS gene was drived.The analysis on promoter sequence by online promoter forecasting tool PLACE and PlantCARE showed that sequence contained a variety of specific expression elements,such as light and various hormones and stressinduced elements.GUS histochemical staining was used to explore the role of the promoter in plant growth regulation.This study had laid a good foundation for GmGAMYB1 characteristics temporal and spatial expression.

相似文献/References:

[1]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(02):596.
[2]郑 旭. 不同浓度6-BA对大豆叶片碳代谢相关生理指标的影响[J].大豆科学,2013,32(06):858.
　ZHENG Xu. Effects of Different Concentrations of 6-BA on Carbon Metabolism Related Indicators in Soybean Leaves[J].Soybean Science,2013,32(02):858.

备注/Memo

 国家自然科学基金（31101169, 30671318，31271748）；黑龙江省教育厅大豆分子设计团队项目；黑龙江省教育厅省高校青年学术骨干项目；东北农业大学博士基金。

更新日期/Last Update: 2014-01-23