[1]徐美蓉,李玉芳,董煚,等.大豆异黄酮超声波辅助提取条件优化的研究[J].大豆科学,2013,32(01):102-106.[doi:10.3969/j.issn.1000-9841.2013.01.024]
　XU Mei-rong,LI Yu-fang,DONG Jiong,et al.Optimization of Soy Isoflavone Extraction Assisted with Ultrasonic[J].Soybean Science,2013,32(01):102-106.[doi:10.3969/j.issn.1000-9841.2013.01.024]
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大豆异黄酮超声波辅助提取条件优化的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 102-106 栏目: 出版日期: 2013-02-25
Title:
Optimization of Soy Isoflavone Extraction Assisted with Ultrasonic
文章编号:
1000-9841(2013)01-0102-05
作者:
徐美蓉;李玉芳;董煚;寇向龙
甘肃省农业科学院 质量与检测标准研究所，甘肃 兰州 730070
Author(s):
XU Mei-rong; LI Yu-fang; DONG Jiong; KOU Xiang-long
Quality and Testing Standards Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China
关键词:
响应面法; 大豆异黄酮; 超声波提取
Keywords:
Response surface method; Soy isoflavone; Ultrasonic assisted extraction
分类号:
TS201.1
DOI:
10.3969/j.issn.1000-9841.2013.01.024
摘要:
以大豆为原料,甲醇为浸提溶剂,5种大豆异黄酮提取率为指标,利用超声波辅助提取,通过单因素实验初步确定提取工艺条件。然后在单因素实验的基础上采用3因素3水平的响应面分析法,建立了总大豆异黄酮含量与各影响因子的回归方程,并得到以总大豆异黄酮提取率为响应值的响应面图和等高线图,进而得出最佳提取工艺条件为固液比1∶50、甲醇提取液浓度85%、提取时间45 min。在此条件下提取2次,大豆异黄酮的总提取率可达0.308%。
Abstract:
The objective of this study was to optimize the extraction conditions of soybean isoflavone,taking soybean as raw material,using the methanol as extraction solvent and ultrasonic assisted extraction method.The extraction parameters was preliminary determined through the single factor experiment.Then the 3 factors 3 levels of response surface analysis method was established using Design-Expert 7 experimental design software,and got the response surface chart and contour map with the response value of the total soybean isoflavones extraction efficiency.The result showed that the best extraction technology conditions was at the ratio of material and solvent 1∶50,with 85% methanol extract concentration and extracting for 45 min.On this condition extracting for 2 times,total soybean isoflavone extraction ratio could reach 0.308%.

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备注/Memo

第一作者简介：徐美蓉（1978-），女，在读硕士，研究方向为农产品质量标准与检测技术研究。E-mail：xxxchz@qq.com。

通讯作者：李玉芳（1964-），女，高级实验师，从事农产品安全及品质检测技术研究。E-mail：gs_lyf@163.com

更新日期/Last Update: 2013-02-20