[1]张思佳,钱秀娟,李春杰,等.昆虫病原线虫对大豆田八字地老虎幼虫致病力的研究[J].大豆科学,2013,32(01):63-71.[doi:10.3969/j.issn.1000-9841.2013.01.015]
　ZHANG Si-jia,QIAN Xiu-juan,LI Chun-jie,et al.Pathogenicity of Entomopathogenic Nematode on Xestia c-nigrum in Soybean Field[J].Soybean Science,2013,32(01):63-71.[doi:10.3969/j.issn.1000-9841.2013.01.015]
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昆虫病原线虫对大豆田八字地老虎幼虫致病力的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 63-71 栏目: 出版日期: 2013-02-25
Title:
Pathogenicity of Entomopathogenic Nematode on Xestia c-nigrum in Soybean Field
文章编号:
1000-9841(2023)01-0063-05
作者:
张思佳1; 2; 钱秀娟3; 李春杰1; 潘凤娟1; 许艳丽1
1.中国科学院 东北地理与农业生态研究所 黑土区农业生态院重点实验室，黑龙江 哈尔滨 150081；
2.中国科学院 研究生院,北京 100049；
3.甘肃农业大学 草业学院，甘肃 兰州 730070
Author(s):
ZHANG Si-jia1; 2; QIAN Xiu-juan3; LI Chun-jie1; PAN Feng-juan1; XU Yan-li1
1.Key Laboratory of Malisons Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,Heilongjiang;
2.Graduate University of Chinese Academy of Sciences,Beijing 100049;
3.Pratacultural College,Gansu Agricultural University,Lanzhou 730070,Gansu,China
关键词:
昆虫病原线虫; 八字地老虎幼虫; 生物测定; 致病力
Keywords:
Entomopathogenic nematode;  Xestia c-nigrum; Bioassay; Pathogenicity
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.015
摘要:
应用斯氏线虫(Steinernema carpocapsae)的4个品系和异小杆线虫(Heterorhabditis bacteriophora)的3个品系,以滤纸为介质对八字地老虎三龄和五龄幼虫进行致病力测定,以筛选出对八字地老虎寄生效果较好的昆虫病原线虫品系及最佳防治龄期。生物测定结果表明,7个不同昆虫病原线虫品系均对八字地老虎三龄幼虫具有致病力,其中斯氏线虫Sc-2的致死速度最快,侵染24、36和72 h的校正死亡率一直高于其他线虫品系,96 h时校正死亡率达到100%。Sc-2侵染五龄幼虫24、36、72、96和120 h校正死亡率也一直高于其他线虫品系。可以看出Sc-2是对八字地老虎幼虫最敏感的昆虫病原线虫品系。但随着八字地老虎幼虫发育该线虫的致病力减弱,即Sc-2对五龄幼虫的校正死亡率低于三龄幼虫。八字地老虎蛹对昆虫病原线虫7个品系都不敏感,侵染后可促进蛹提前羽化。因此,田间防治八字地老虎时三龄幼虫期效果最好。
Abstract:
Xestia c-nigrum L.is one of the pests seriously damaging soybean and other agricultural crops.In order to achieve entomopathogenic nematode with higher pathogenicity and optimum control stage of Xestia c-nigrum,four strains of Steinernema carpocapsae and three strains of Heterorhabditis bacteriophora were used to test pathogenicity against juvenile 3 and 5 of Xestia c-nigrum on filter paper.The results showed that seven strains of entomopathogenic nematode had pathogenicity on juvenile 3 of Xestia c-nigrum,and the pathogenicity of Steinernema carpocapsae was stronger than that of? S.glaseri and Heterorhabditis bacteriophora. Steinernema carpocapsae- 2(Sc-2)had the fastest lethal speed on juvenile 3 of Xestia c-nigrum;the half lethal time was 36 h,and the half lethal time of other strains of entomopathogenic nematode was longer than 48 h.The correct cumulative mortality of juvenile 3 infected by Sc-2 was higher than that caused by other nematode stains when juvenile 3 was infected for 24,36 and 72 h,and the correct cumulative mortality reached at 100% when juvenile 3 was infected for 96 h.The correct cumulative mortality of juvenile 5 infected by Sc-2 was higher than that caused by other nematode strains when juvenile 5 was infected for 24,36,72,96 and 120 h.The pathogenicity of Sc-2 was the strongest on Xestia c-nigrum among the seven nematode strains.The pathogenicity of Sc-2 decreased as Xestia c-nigrum grew up,and the correct mortality of juvenile 3 was higher than that of juvenile 5 when they were infected by Sc-2.The pupal of Xestia c-nigrum was insensitive to the seven strains of the entomopathogenic nematode,and the eclosion of Xestia c-nigrum was promoted by the entomopathogenic nematode.Juvenile 3 was considered as the optimum control stage of Xestia c-nigrum to entomopathogenic nematode.

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备注/Memo

基金项目：中国科学院知识创新工程重要方向项目（KSCX2-YW-N-092）;国家农业科技成果转化资金项目（2007GB24910482）。

第一作者简介：张思佳（1988-），女，在读硕士，研究方向为生物防治。E-mail：zhangsijia0526@126.com。
通讯作者：许艳丽（1958-），女，研究员，博士生导师，主要从事农田有害生物防治研究。E-mail：xyll@neigaehrb.ac.cn。

更新日期/Last Update: 2013-02-20