[1]戴嘉乐,马 建,付永平,等.大豆7S蛋白β亚基基因RNAi表达载体构建[J].大豆科学,2012,31(06):1021-1023.[doi:10.3969/j.issn.1000-9841.2012.06.035]
　DAI Jia-le,MA Jian,FU Yong-ping,et al.Construction of the RNAi Expression Vector of Soybean 7S Protein β-subunit Gene[J].Soybean Science,2012,31(06):1021-1023.[doi:10.3969/j.issn.1000-9841.2012.06.035]
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大豆7S蛋白β亚基基因RNAi表达载体构建

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年06期 页码: 1021-1023 栏目: 出版日期: 2012-12-30
Title:
Construction of the RNAi Expression Vector of Soybean 7S Protein β-subunit Gene
文章编号:
1000-9841（2012）06-1021-03
作者:
戴嘉乐; 马 建; 付永平; 曲 静; 王丕武
吉林农业大学 农学院，吉林 长春 130118
Author(s):
DAI Jia-le; MA Jian; FU Yong-ping; QU Jing; WANG Pi-wu
Agronomy College,Jilin Agricultural University,Changchun 130118,Jilin,China
关键词:
RNA干扰; 7S蛋白β亚基; 表达载体
Keywords:
RNA interference; 7S protein β-subunit; Expression vector
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.035
文献标志码:
A
摘要:
以大豆7S蛋白β亚基基因为靶基因(基因编号：AB030840)，利用RT-PCR克隆得到大豆7S蛋白β亚基基因核心序列398 bp，构建了以抗除草剂基因BAR为筛选标记的安全植物RNAi表达载体BAR-7αp-β。分子生物学检测表明7S蛋白β亚基基因的表达载体构建成功。研究结果为应用RNA干扰技术降低大豆过敏原，提高大豆蛋白品质奠定了基础。
Abstract:
In this study we used β-subunit gene of 7S protein as the target genes(Gene number:AB030840),then obtained 398 bp core sequence of β-subunit gene by RT-PCR and constructed RNAi expression safe plant vector BAR-7αp-β，with a herbicide resistant BAR gene as selection marker.The result of PCR,molecular biology detection demonstrated that gene expression vector of 7S protein β-subunit was successfully constructed.This study provided foundation for the application of RNA interference technology to reduce soybean allergens and improve quality of soybean proteins.

参考文献/References:

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备注/Memo

第一作者简介：戴嘉乐（1985-），男，在读硕士，研究方向为植物基因工程。E-mail：dale8520@163.com。

通讯作者：王丕武（1958-），教授 ，博士生导师，主要从事农业生物技术和作物遗传育种研究。E-mail:peiwuw@yahoo.com.cn。

更新日期/Last Update: 2014-08-20