Department of Life Science,Zhoukou Normal University,Zhoukou 00,et al.Effects of Heavy Metal and Organic Compound Contamination on Physiological Character and DNA Damage of Soybean Seedlings[J].Soybean Science,2012,31(06):915-919.[doi:10.3969/j.issn.1000-9841.2012.06.012]
重金属及有机物复合污染对大豆生理特性和DNA损伤效应的影响
- Title:
- Effects of Heavy Metal and Organic Compound Contamination on Physiological Character and DNA Damage of Soybean Seedlings
- 文章编号:
- 1000-9841（2012）06-0915-05
- Keywords:
- Wild soybean; Heavy metal; Physiological property; DNA damage
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用沙培方法研究了模拟复合污水（汞、铜、镉、苯、二甲苯和苯酚）对野生大豆和栽培大豆幼苗的生理特性以及DNA损伤效应的影响。结果表明：经污水处理后，大豆幼苗叶片中叶绿素含量、DNA提取量均下降；DNA增色效应、可溶性蛋白、可溶性糖、脯氨酸、丙二醛含量均上升。其中野生大豆ZKDC的叶绿素含量、DNA提取量下降幅度最小，可溶性蛋白、可溶性糖、脯氨酸含量上升幅度最大，DNA增色效应与丙二醛含量上升幅度最小。因此供试材料中野生大豆ZKDC的耐污能力最强。
- Abstract:
- The effects of sewage(Hg,Cu,Cd,benzene,xylene and phenol)irrigation on physiological character and DNA damage of Glycine soja and Glycine max were evaluated by sand culture tests.The results showed that the contents of chlorophyll and the extracts of DNA of soybean seedlings were significantly decreased than those of control.The hyperchromicity of DNA and the contents of soluble protein,soluble sugar,proline and MDA were significantly increased.Compared with the other tested materials,the Glycine soja‘ZKDC’had the smallest decline for chlorophyll content and DNA extracts amount,the largest rise for soluble protein,soluble sugar and proline content,and the smallest rise for hyperchromicity of DNA and MDA content.In summation,‘ZKDC’ has the best torlerance for sewage pollution.
参考文献/References:
[1]陈怀满.土壤中化学物质的行为与环境质量[M].北京：科学出版社，2002，8（2）：183-188.（Chen H M.Chemical substantial activity and environment quality in soil[M].Beijing:Science Press,2002，8（2）：183-188.）
[2]高雪峰，敖恩宝力格.重金属Pb、Hg污染对3种小麦种子的萌发及抗氧化活性的影响[J].种子，2012，31（2）：17-20.（Gao X F，Aoen B G.Effect of Pb,Hg pollution on germination and antioxidant activity of three kinds of wheat seeds[J].Seed，2012，31（2）：17-20.）
[3]伍钧，吴传星，孟晓霞，等.重金属低积累玉米品种的稳定性和环境适应性分析[J].农业环境科学学报，2011，30（11）：2160-2167.（Wu Y，Wu C X，Meng X X，et al.The analysis of stability and adaptability on low accumulation of heavy metals in various cultivars of Zea mays[J].Journal of Agro-Environment Science，2011，30（11）：2160-2167.）
[4]文晓慧，蔡昆争，葛少彬，等.硅对镉和锌复合胁迫下水稻幼苗生长及重金属吸收的影响[J].华北农学报，2011，26（5）：153-158.（Wen X H，Cai K Z，Ge S B，et al.Effects of silicon on plant growth in rice seedlings under and heavy metal absorption Cd and Zn stress[J].Acta Agriculturae Boreali-Sinica，2011，26（5）：153-158.）
[5]白瑞琴，孟海波，周爽，等.重金属Cd对两个马铃薯品种生长发育的影响[J].华北农学报，2012，27（1）：168-172.（Bai R Q，Meng H B，Zhou S，et al.Effects of cadmium on growth and development of two potato varieties[J].Acta Agriculturae Boreali-Sinica，2012，27（1）：168-172.） [6]李三相，李勃，兰小平，等.Cr6+胁迫对大豆幼苗生理生化特性的影响[J].大豆科学，2010，29（5）：777-780.（Li S X，Li B，Lan X P，et al.Effects of Cr6+?stress on physiological and biochemical parameters of soybean seedlings[J].Soybean Science，2010，29（5）：777-780.）
[7]李建武，余瑞元，袁明秀，等.生物化学实验原理和方法[M].北京：北京大学出版社，2002:264-280.（Li J W,Yu R Y,Yuan M X,et al.Biochemistry experiment principle and method[M].Beijing：Peking University Press，2002:264-280.）
[8]葛才林，杨小勇，孙锦荷，等.重金属胁迫引起的水稻和小麦幼苗DNA损伤[J].植物生理与分子生物学学报，2002，28（6）：419-424.（Ge C L,Yang X Y,Sun J H,et al.DNA damage caused by heavy metal stress in rice and wheat seedlings[J].Journal of Plant Physiology and Molecular Biology，2002，28（6）：419-424.）
[9]李合生.植物生理生化实验原理和技术[M].北京：高等教育出版社，2003.（Li H S.Plant physiology and biochemistry experiment principle and technology[M].Beijing：Higher Education Press，2003.）
[10]刘萍，李明军.植物生理实验技术[M].北京：科学出版社，2008.（Liu P，Li M J. Plant physiology experimental technology[M].Beijing：Science Press，2008.） [11]徐楠，施国新，曾晓敏，等.Hg2+胁迫对浮萍体细胞DNA一级结构和抗氧化酶系统的损伤[J].植物生态报，2003，27（3）：299-303.（Xu N，Shi G X，Zeng X M，et al.Damage to DNA primary structure and antioxidant enzymes in lemna minor?induced by Hg2+[J].Acta Phytoecologica Sinica，2003，27（3）：299-303.）
[12]常云霞，王红星，陈龙.水杨酸对锌胁迫下小麦幼苗生长抑制的缓解效应[J].西北植物学报，2011，31（10）：2052-2056.（Chang Y X，Wang H X，Chen L.Salicylic acid mitigating the inhibition of zinc stress to wheat seedling growth[J].Acta Botanica Boreali-Occidentalia Sinica，2011，31（10）：2052-2056.）
[13]冯亚楠，李璨，冯乃杰，等.不同植物生长调节剂浸种对大豆幼苗子叶碳代谢的影响[J].大豆科学，2009，28（6）：1016-1020.（Feng Y N，Li C，Feng N J，et al.Effects of seed soaking with plant growth regulators（PGRs）on the carbon metabolism of soybean seedling cotyledon[J].Soybean Science,2009,28（6）:1016-1020.）
[14]杨卫民,刘宝琦,张世珍.甘露醇、过氧化氢和氯化钠处理对黑豆苗期抗旱性的影响[J].大豆科学，2010，29（2）：350-353.（Yang W M，Liu B Q，Zhang S Z.Effect of mannitol,hydrogen peroxide and chloride treatment on drought resistance of black soybean seedling[J].Soybean Science，2010，29（2）：350-353.）
[15]郑世英，郑建峰，张秀玲，等.NaCl胁迫对野生及栽培大豆渗透调节物质含量的影响[J].大豆科学，2011，30（5）：786-789.（Zheng S Y，Zheng J F，Zhang X L，et al.Effect of NaCl stress on the content of osmotic regulation substances in Glycine soja?and Glycine max[J].Soybean Science，2011，30（5）：786-789.）
[16]孙富，杨丽涛，谢晓娜，等.低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响[J].作物学报，2012，38(4)：732-739.（Sun F，Yang L T，Xie X N，et al.Effect of chilling stress on physiological metabolism in chloroplasts of seedlings of sugarcane varieties with different chilling resistance[J].Acta Agronomica Sinica，2012，38（1）：732-739.）
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备注/Memo
基金项目：河南省教育厅自然科学研究计划资助项目（2009B180029，2011B180057）； 周口师范学院生物化学与分子生物学重点学科建设项目。