WANG Yan,WU Lin,SUN Meng-yang,et al.Analysis of Gene Expression Underlying Soybean Isoflavone Synthesis Relative Enzymes at Different Growth Stages[J].Soybean Science,2012,31(06):887-893.[doi:10.3969/j.issn.1000-9841.2012.06.006]
不同生育时期大豆异黄酮合成相关酶基因表达的分析
- Title:
- Analysis of Gene Expression Underlying Soybean Isoflavone Synthesis Relative Enzymes at Different Growth Stages
- 文章编号:
- 1000-9841（2012）06-0887-07
- 关键词:
- 大豆异黄酮; 异黄酮合成相关酶基因; Real-time PCR; 相对表达量
- Keywords:
- Soybean isoflavone; Isoflavone synthesis relative enzyme genes; Real-time PCR; Relative expression quantity
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 通过Real-time PCR分析了不同生育时期7个大豆异黄酮合成相关酶基因（PAL,C4H,4CL,CHS,CHI,IFS和F3H）的相对表达情况。并利用高效液相色谱法测定了籽粒发育过程中豆荚（含籽粒）中异黄酮及其组分的含量，同时利用SAS 8.2分析了F5∶11重组自交系群体（RILs）豆荚中异黄酮含量与部分基因相对表达量间的相关性。结果表明：从营养生长进入生殖生长阶段，部分基因（PAL,CHI,IFS和F3H）的相对表达量普遍有显著提高；PAL,C4H,CHS和IFS基因在叶片中的相对表达量高峰出现在R1期,大部分基因在2个品种豆荚中相对表达量差异显著的时期为R6期。在R6期,豆荚PAL基因的相对表达量与染料木素(GT)和总异黄酮含量(TI)呈显著正相关。CHS基因的相对表达量与大豆黄素(DZ)、染料木素(GT)和总异黄酮含量（TI）呈显著正相关,但是与黄豆黄素（GC）呈显著负相关，IFS基因的相对表达量与大豆黄素(DZ)含量呈显著正相关；F3H基因的相对表达量与所有异黄酮成分均呈显著负相关。研究明确了相应基因的表达情况，对于理解异黄酮含量变异的遗传机制具有重要意义。
- Abstract:
- Real-time PCR was used to measure the relative expression quantity of seven isoflavone synthetic enzyme related genes(PAL,C4H,4CL,CHS,,IFCHIS and F3H)in high isoflavonecv.Zhongdou27,low isoflavone cv. Jiunong20,as well as their derived 130 F5∶11 RILs.High performance liquid chromatography(HPLC)was adopted to survey isoflavone content in pod at different growth stages,at the same time,correlations between total/individual isoflavone content and the expression of partial genes in F5∶11 RILs were performed using SAS 8.2.The relative expression quantity of（PAL，CHI，IFS and F3H was generally increased in reproductive stage than vegetative stage.Relative expression quantity of PAL,C4H,CHS and IFS gene in leaf appeared a peak value at R1 stage,and the expression quantity of most selected genes appeared significant difference in pod between two variety was at R6 stage.At R6 stage,the relative expression quantity of PAL gene manifested positive correlation with genistein(GT)and total isoflavone content(TI);CHS exhibited positive relevance with daidzein(DZ),GT and TI but negative with glycitein(GC);IFS displayed positive correlation with DZ;F3H showed negative correlation with individual and total isoflavone contents.Results will faciliate the understanding of genetic mechanism of the variation underlying isoflavone content.
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备注/Memo
基金项目：农业部大豆产业技术体系资助项目（CARS-04-PS04）；国家“十二五”科技支撑计划课题（2011BAD35B06）；973前期项目（2012CB126311）；国家自然基金项目（31201227）；中国博士后项目（20110491024）；黑龙江省博士后项目(LBH11220)；黑龙江省骨干教师项目的资助项目（1252G014）。