[1]吴明霞,陈锡雄,阮少江.响应面法优化啤酒糟培养基生产蛋白饲料的研究[J].大豆科学,2012,31(05):842-845.[doi:10.3969/j.issn.1000-9841.2012.05.034]
　WU Ming-xia,CHEN Xi-xiong,RUAN Shao-jiang.Optimization of Microbial Media of Brewer Spent Grains of Protein Feed by Response Surface Methodology[J].Soybean Science,2012,31(05):842-845.[doi:10.3969/j.issn.1000-9841.2012.05.034]
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响应面法优化啤酒糟培养基生产蛋白饲料的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年05期 页码: 842-845 栏目: 出版日期: 2012-10-25
Title:
Optimization of Microbial Media of Brewer Spent Grains of Protein Feed by Response Surface Methodology
文章编号:
1000-9841（2012）05-0842-04
作者:
吴明霞; 陈锡雄; 阮少江
宁德师范学院 生物工程系，福建 宁德 352100
Author(s):
WU Ming-xia; CHEN Xi-xiong; RUAN Shao-jiang
Department of Biological Engineering,Ningde Normal University,Ningde 352100,Fujian,China
关键词:
响应面法; 啤酒糟; 培养基; 优化
Keywords:
Response surface methodology(RSM); Drewer spent grains; Fermentation medium; Optimization
分类号:
TS262.5
DOI:
10.3969/j.issn.1000-9841.2012.05.034
文献标志码:
A
摘要:
选取啤酒糟量、麸皮量和硫酸铵量为因子，用响应面法对生产蛋白饲料的培养基进行优化。在单因素试验的基础上,根据Box-Benhnken中心组合方法进行三因素三水平试验。结果表明,最佳工艺条件为啤酒糟88.5%、麸皮7.5%、硫酸铵1%。在此条件下得到的真蛋白含量为37.64%。
Abstract:
To optimize culture medium of the production of protein feed,the effect of the amount of wheat bran,lees and ammonium sulfate weight were studied with Box-Behnken design.The optimum extraction conditions were obtained as follows:drewer spent grains 88.5%，wheat bran 7.5%,(NH4)2SO41%.Under the optimal conditions,the true protein content was 37.64%,which agreed well with predictive value,indicating the optimized process was feasible.

参考文献/References:

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[6]赵志水,蒲彬,贺玉凤,等.响应面法优化新疆红枣总黄酮乙醇提取工艺[J].中国酿造,2012(1):88-90.(Zhang Z S,Pu B,He Y F,et al.Optimization of ethanol extraction process of total flavonoids from Xinjiang jujube by response surface methodology[J].China Brewing,2012(1):88-90.)


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备注/Memo

基金项目：宁德师范学院“服务海西建设”项目（2010H308）。

第一作者简介：吴明霞（1979-），女，硕士，讲师，研究方向为微生物代谢调控。E-mail：wuming0224@163.com。

更新日期/Last Update: 2014-08-19