[1]杨靓,李翔宇,高鹏,等.野生大豆胁迫应答LRR类受体蛋白激酶基因的克隆及其表达特性分析[J].大豆科学,2012,31(05):718-724.[doi:10.3969/j.issn.1000-9841.2012.05.006]
　YANG Liang,LI Xiang-yu,GAO Peng,et al.Isolation and Expression Pattern Analysis of LRR Kinase Gene Response to Osmotic Stress from Glycine soja [J].Soybean Science,2012,31(05):718-724.[doi:10.3969/j.issn.1000-9841.2012.05.006]
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野生大豆胁迫应答LRR类受体蛋白激酶基因的克隆及其表达特性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年05期 页码: 718-724 栏目: 出版日期: 2012-10-25
Title:
Isolation and Expression Pattern Analysis of LRR Kinase Gene Response to Osmotic Stress from Glycine soja
文章编号:
1000-9841（2012）05-0718-07
作者:
杨靓1; 李翔宇2; 高鹏3; 陈庆园4; 郭玉双4
1.福建农林大学 植物病毒研究所,福建省植物病毒学重点实验室，福建 福州 350002；
2.黑龙江省农业科学院 作物育种研究所，黑龙江 哈尔滨 150086；
3.东北农业大学 园艺学院，黑龙江 哈尔滨 150030；
4.贵州省烟草科学研究所，贵州 贵阳 550083
Author(s):
YANG Liang1; LI Xiang-yu2; GAO Peng3; CHEN Qing-yuan4; GUO Yu-shuang4
1.Key Laboratory of Plant Virology of Fujian Province,Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian;
2.Crop Breeding Instituce,Heilongjiang Academy of Agricultural Science,Harbin 150086,Heilongjiang;
3.College of Horticulture,Northeast Agricultural University,Harbin 150030,Heilongjiang;
4.Guizhou Institute of Tobacco Science,Guiyang 550083,Guizhou,China
关键词:
野生大豆; LRR-RLK; 渗透胁迫
Keywords:
Glycine soja; LRR-RLK; Osmotic stress
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.05.006
文献标志码:
A
摘要:
从前期构建的野生大豆在高盐、低温、干旱早期的基因表达谱中，选取了在4℃胁迫早期（1 h）上调表达的LRR-RLK的EST，通过SMART和RACE结合的方法获得了GsLRPK的全长序列：该基因全长2 264 bp，共编码714个氨基酸。生物信息学分析表明其氮端含有1个LRR N-terminal domain及串联排列的LRR-Motif；碳端含有丝/苏氨酸蛋白激酶催化结构域的11个亚基。此外，GsLRPK蛋白氮端22个氨基酸为可能的信号肽序列，并且存在1个跨膜结构域，很可能定位于细胞质膜或细胞器膜。半定量PCR分析显示该基因可受干旱、ABA、4℃低温及高盐胁迫的诱导，可能作为一个“关键节点”在胁迫信号传导通路中发挥重要作用。
Abstract:
One EST induced by cold represents a leucine-rich repeat receptor-like kinase(LRR-RLK)gene was identified according to the gene expression profiles of Glycine soja leaves under osmotic stresses previously constructed in our laboratory.The full-length cDNA was successfully retrieved by RACE,which was 2 264 bp in length and contains a complete ORF encoding 714 amino acids.The bioinformatics analysis showed that,the GsLRPK protein had an N-terminal signal sequence(residues 1 to 22),extracellular leucine-rich repeats,a single transmembrane region,and a cytoplasmic catalytic domain,considered crucial for kinase activity.What’s more,semi-quantity PCR result showed that the GsLRPK was regulated by different stresses treatment,such as drought,ABA,4℃ cold and high salinity,and could function in the signaling of osmotic stresses.

参考文献/References:

[1]Yang L,Ji W,Gao P,et al.GsAPK an ABA-activated and calcium-independent SnRK2-type kinase from G.soja mediates the regulation of plant tolerance to salinity and ABA stress[J].PLoS ONE,2012,7(3):e33838.doi:10.1371/journal.pone.0033838.

[2]Zhou Q Y，Tian A G，Zou H F,et al.Soybean WRKY-type transcription factor genes,GmWRKY13,GmWRKY21 and GmWRKY54,confer differential tolerance to abiotic stresses in transgenic Arabidopsis?plants[J].Plant Biotechnology Journal,2008,6(5):486-503.

[3]Yang L,Ji W,Zhu Y M,et al.GsCBRLK,a calcium/calmodulin-binding receptor-like kinase,is a positive regulator of plant tolerance to salt and ABA stress[J].Journal of Experimental Botany,2010,61(9):2519-2533.

[4]Yoshioka K,Shinozaki K.Signal crosstalk in plant stress responses[M].Wiley-Blackwell,Malden,2009.

[5]Morris E R,Walker J C.Receptor-like protein kinases:the keys to response[J].Current Opinion in Plant Biology,2003,6:339-342.

[6]Shiu S H,Karlowski W M,Pan R,et al.Comparative analysis of the receptor-like kinase family in Arabidopsis?and rice[J].Plant Cell,2004,16:1220-1234.

[7]Kobe B,Kajava A V.The leucine-rich repeat as a protein recognition motif[J].Current Opinion in Plant Biology,2001,11:725-732.

[8]Belkhadir Y,Subramaniam R,Dangl J L.Plant disease resistance protein signaling:NBS-LRR proteins and their partners[J].Current Opinion in Plant Biology,2004,7:391-399.

[9]Song W Y,Wang G L,Chen L L,et al.A receptor kinase-like protein encoded by the rice disease resistance gene Xa21[J].Science,1995,270:661-667.

[10]Wang G L,Wu C,Zeng L,et al.Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X.oryzae pv.Oryzae[J].Theoretical and Applied Genetics,2004,108:379-384.

[11]Wang Z Y,Seto H,Fujioka S,et al.BRI1 is a critical component of a plasma-membrane receptor for plant steroids[J].Nature,2001,410(6826):380-383.

[12]He Z,Wang Z Y,Li J,et al.Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1[J].Science,2000,288(3):2360-2363.

[13]Yuriko O,Kyonoshin M,Motoaki S Y,et al.Leucine-rich repeat receptor-like kinase1 is a key membrane-bound regulator of abscisic acid early signaling in Arabidopsis[J].Plant Cell,2005,17:1105-1119.

[14]Junga E H,Junga H W,Lee S C,et al.Identification of a novel pathogen-induced gene encoding a leucine-rich repeat protein expressed in phloem cells of Capsicum annuum[J].Biochimica et Biophysica Acta.2004,1676(3):211-222.

[15]李勇,朱延明,李杰,等.基于Linux的cDNA文库序列分析平台的构建与应用[J].生物信息学,2005,3:124-127.(Li Y,Zhu Y M,Li J,et al.Construction and application of cDNA library sequence analysis platform based on Linux[J].China Journal of Bioinformatics,2005,3:124-127).

[16]周宜君,冯金朝,马文文,等.植物抗逆分子机制研究进展[J].中央民族大学学报(自然科学版),2006,15(2):169-176.(Zhou Y J,Feng J C,Ma W W,et al.Progress of molecular mechanism of stress resistance in plants[J].Journal of the Central University for Nationalities(Natural Sciences Edition),2006,15(2):169-176).

[17]杨晓慧,蒋卫杰,魏珉,等.提高植物抗盐能力的技术措施综述[J].中国农学通报,2006,22(1):88-91.(Yang X H,Jiang W J,Wei M,et al.The technical approaches of Improving the plant salt-resistant ability[J].Chinese Agricultural Science Bulletin,2006,22(1):88-91).

[18]葛瑛.野大豆碱胁迫转录谱与基因组整合分析[D].哈尔滨：东北农业大学,2010.(Ge Y.Integration of microarray and genome analysis in alkaline-stressed Glycine soja[D].Harbin:Northeast Agricultural University,2010).

[19]周国安.大豆抗逆基因GmUBC2、GmPK和GmNHX2分离与功能研究[D].北京:中国农业科学院,2009.(Zhou G A.Isolation and function analysis of stress-responsive genes GmUBC2,GmPKand GmNHX2 from soybean(Glycine MaxL.Merr.)[D].Beijing:Chinese Academy of Agricultural Sciences,2009).

[20]Hwang S G,Kim D S,Jang C S.Comparative analysis of evolutionary dynamics of genes encoding leucine-rich repeat receptor-like kinase between rice and Arabidopsis [J].Genetica,2011,139(8):1023-1032.

[21]Huang C F,Yamaji N,Ono K,et al.A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots[J].The Plant Journal,2012,69(4):565-576.


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备注/Memo

基金项目：福建省自然科学基金项目(2011J05051)；福建省教育厅科技项目(JA11080)。

第一作者简介：杨靓（1981-），女，博士，讲师，研究方向为分子病毒学。E-mail：yangliang.fafu@139.com。
通讯作者：郭玉双（1981-），男，博士，研究方向为烟草生物技术。E-mail：yshguo@126.com。

更新日期/Last Update: 2014-08-18